Abstract:

[Abstract]   Objective   To investigate the expression and clinical significance of four histamine receptors: H1R, H2R,
H3R and H4R in urinary bladder of patients with ketamine-induced cystitis. Methods   Immunohistochemical S-P method was used to detect the expression levels of histamine receptors: H1R, H2R, H3R and H4R in bladder tissues of10patients with ketamine-induced cystitis (experimental group) and distal tissue away from bladder tumors of10patients with cystectomy (control group). The average optical density (OD) values of four kinds of different histamine receptors were separately calculated by Imagepro-plus 6.0in two groups. At the same time, mast cells were marked by toluidine blue special dyeing and were counted.  Results   Comparing with control group, the expression levels of H1R, H2R, and H4R were significantly increased in experimental group (P <0.05). Mast cells diffused interstitial bladder infiltrates (P<0.05). There was no significant difference in the expression of H3R in two  groups. Conclusion  Mast cells, H1R, H2R, and H4R are closely related to the ketamine-induced cystitis, which may be new diagnostic indicators and new treatment targets of ketamine-induced cystitis.

Key words: ketamine, cystitis, receptors, histamine, mast cells, immunohistochemistry