Abstract:

[Abstract]   Objective   To study the effect of the cord blood stem cell transplantation therapy in patients with severe
hepatitis.   Methods    Forty patients with severe hepatitis were randomly divided into control groups (n=20) and observed group (n=20). The patients in two groups were treated with the conventional liver protective therapy respectively, such as compound glycyrrhizin and glutathione. The patients in observed groups were performed with the cord blood stem cell trans?plantation therapy. After isolation and purification, the cells were transplanted into the liver via hepatic artery and then were transplanted via peripheral veins. The biochemical indices of albumin (ALB), prothrombin activity (PTA)，alpha-fetoprotein （AFP）and clinical symptoms were detected at different times after therapy.   Results   After4-week therapy, the levels of PTA（%）and AFP（μg/L）were significantly increased in observed group compared with those of control group (53.56±5.15vs48.26±5.53，92.16±9.09vs43.57±11.33，P<0.01). After8-week therapy, the levels of alanine aminotransferase (ALT), totalbilirubin (TBIL) and AFP were significantly decreased in observed group than those of control group. The level of PTA was sig?nificantly higher in observed group than that of control group (62.61±4.35vs54.95±3.01，P<0.05orP<0.01). There were nosignificant differences in levels of ALB and blood ammonia between two groups (P>0.05). There were no serious complications and adverse reactions in two groups of patients. There was no significant difference in mortality between two groups (20%vs35%,χ²=0.818 P>0.05).   Conclusion   Cord blood stem cell therapy has a good effect on treating severe hepatitis, which is safe and no significant adverse reactions, and can be used as a new method for treating the severe hepatitis.

Key words: cord blood stem cell transplantation, alpha-fetoproteins, serum albumin, alanine transaminase, severe hepatitis