Abstract: To study the effects of human growth hormone on pro liberation and apoptosis is of human breast neoplasm cells of human in vivo. Methods : The nude mice randomly were divided into four groups and each group 10 mouse. Nude mice were randomly divided into control group, recombinant human growth hormone (rhGH) group, 5-fluorouracil (5-FU) group and rhGH+ 5-FUgroup after human gastric cancer engraft model of nude ice as successfully established. IP give medicine in each group, and observe the influence of drugs on tumor growth after two weeks. The cell cycle, carryon proliferation antigen, apoptosis index (AI) of human breast cancer cell were investigated by flow cytometry, immunohistochemical detection, and terminal dioxin elatedly transferees-me dieted deoxyurdine troposphere biotin nick end labeling (TUNEL) respectively. Results: Cells in S phase, tumor quality and PCNA of gastric cancer engraft obviously degraded, but A I and G0/G1 phase obviously increased in 5-FU group and rhGH+ 5-FU groups compared with control and rhGH groups (P < 0.05); Compared with 5- FU group, rhGH + 5- FU group of tumor quality, PI and G2M period decline rates significantly better than 5- FU group, G0/G1 phase obviously better than the rate 5- FU group (P < 0.05). Between rhGH group and control group, there was no difference in above indices. Conclusion: Exogenous human growth hormone has no obvious effects on pro liberation and apoptosis of human pancreatic cancer cells in vivo. In vitro r-hGH might promote the sensitivity of chemotherapy of 5-FU to MC'F-7 cells

Key words: human growth hormone, human breast neoplasm cells, proliferation, apoptosis