Abstract:

[Abstract]   Objective   To investigate the association between the signaling pathways of MCP-1-pCDH-GFP-trans?
fected cells and deep venous thrombosis (DVT).   Methods   The cultured human umbilical vein endothelial cells (HUVECs)were tested by immunofluorescence and co-immunoprecipitation methods. The constructed MCP-1over-expression/interference vector, and the change of transcription profile were detected by microarray assay and biological information technology analysis.  Results   MCP-1over-expression/interference vector MCP-1-pCDH-GFP/MCP-1-LMP shRNAmir1was constructed and HUVECs were transfected. According to the microarray analysis we found that there were18down-expressed signaling pathways and7up-expressed signaling pathways in MCP-1-pCDH-GFP-transfected cells. There were60downexpressed signaling pathways and15up-expressed signaling pathways in the MCP-1-LMP shRNAmir1transfected cells.   Conclusion   Signaling pathways of MCP-1plays an important role in DVT formation.which may provide us a new way to study molecular mechanism of DVT.

Key words: monocytes, chemotactic factors, endothelium,vascular, umbilical veins, signal transduction, venous thrombosis, monocyte chemoattractant protein-1, human umbilical vein endothelial cells