Abstract: Objective: To explore the inhibitory effect of berbamine on the proliferation of UMR-106 cells and its possible mechanism in vitro. Objective: To explore the inhibitory effect of berbamine on the proliferation of UMR-106 cells and its possible mechanism in vitro. Methods: UMR-106 cells were cultured with different concentrations of berbamine （0,2,4,8,16 and 32 mg/L） for 24, 48 and 72 h ,respectively,and the proliferation were colorimetrically assayed by MTT. UMR-106 cells were interfered with different concentrations of berbamine （0,4,8 and 16 mg/L）for 24h,the morphological changes of UMR-106 cells were observed by laser confoca1 scan microscope after Hoechst33258 staining, the apoptotic rates were detected by flow cytometry （FCM）using Annexin V/PI staining, the cell cycle were detected by FCM using PI staining. Results: Berbamine could obviously inhibit the proliferation of UMR-106 cells in a dose-dependent manner （P < 0.01）.The IC50 value at 24,48 and 72h were 24.69、8.03 and 3.54 mg/L, respectively. There were nuclear condensation and apoptotic body in berbamine treated group. Apoptotic rates of control group and berbamine treated group were （1.64±0.29）% ，（3.58±0.31） %，（6.27±0.47） % and （11.27±1.09） %，respectively.There was a significant difference in apoptotic rate compared with the control group （P < 0.01）. Meanwhile, berbamine can induce cell necrosis .In addition, berbamine significantly increased the percentages of cells in G0/G1 phase and decreased in S phase and G2/M phase compared with the control group （P <0.01）. Conclusion:berbamine can inhibit the proliferation of UMR-106 cells in vitro, inducing cell apoptosis , necrosis and G0/G1 phase arrest may be the mechanism .

Key words: berbamine , osteosarcoma , cell proliferation, apoptosis, cell cycle, in vitro , dose-response relationship, 药物