Abstract: Objective Brain derived neurotrophic factor(BDNF) and its specific tyrosine kinase receptor B(TrkB) are highly correlated to epilepsy. This study mean to explore the regulation of BDNF/TrkB signal pathway in hippocampal neurons by different isoforms of TrkB. Methods The cells were divided into 14 groups.The immunofluorescent technique was used to identificate the hippocampal neurons.Epileptiform discharges were detected by electrophysiological techniques.Western blot assay was used to determine the protein expression of TrkB and p-TrkB of each group. Results Compared with control group, the p-TrkB/TrkB value in control + BDNF group was higher. In epilepsy + BDNF group, the p-TrkB/TrkB value was lower than control+BDNF group, but higher than epilepsy group.The p-TrkB/TrkB value of epilepsy + ALLN + BDNF group was lower than epilepsy + BDNF group. The difference of the p-TrkB/TrkB value between epilepsy + ALLN + BDNF group and epilepsy + ALLN group was not significant.The p-TrkB/TrkB value in epilepsy + anisomycin + BDNF group was higher than both of epilepsy + anisomycin group and epilepsy + BDNF group. Conclusion BDNF can activate BDNF/TrkB signal pathway.The increased expression of TrkB.T can inhibit the activation of BDNF/TrkB signaling in status epilepticus.The change of TrkB.FL protein levels cannot inhibit the BDNF/TrkB signal pathway.

Key words: Epilepsy, Temporal Lobe, BDNF/TrkB signal pathway, Fluorescent Antibody Technique, Blotting, Western