Abstract: [Abstract] Object To verify the genes screend by the PCR chip of cell cycle. Methods The colon cancer cells were randomized into two groups. The test group recoved the stimulation of gastrin,while the control group not .The method of western blot were used to detect the expression of Cacybp/SIP before and after the stimulation of gastrin. Verification of differential expression of CDK8, CKS2 by using real-time quantitative PCR. Results It is found that before the stimulation of human gastrin, CacyBP/SIP is located and expressed in the cytoplasm, and then in both cytoplasm and nucleus after the stimulation of human gastrin. The results of real-time quanntitative PCR of CDK8 and CKS2 gene were consistent with those of microarray detection , The expressions of CDK8 and CKS2 were up-regulated. Conclusion The stimulation of human gastrin can lead to the nuclear translocation of CacyBP/SIP. The results of microarray are reliable and differentially expressed genes screened through gene chip deserve futher study.

Key words: CacyBP/SIP, nuclear translocation, differential expression gene, gastrin