Abstract: 【Abstract】Objective: To investigate the enhanced osteogenesis on bone fracture healing at a segmental bone defect of injecting leptin into intracerebroventricular (ICV) through Foramen magnum. Method: Segmental critical-sized defects were created at the right tibial bone of skeletally mature New Zealand white rabbits. Recombinant rabbit leptin (leptin group) and normal saline (control group) were injected into cerebellomedullary cistern through foramen magnum. Bone fracture healing was evaluated through callus formation and bone mineral density (BMD) by using qCT at 1st, 3rd, 7th days and the 2nd ,3rd week. At the 3rd week, all rabbits were euthanized to collect the right tibia for histomorphology. Result: The result showed that ICV leptin exhibited significantly high osteoinduction activity compared with that of NS group: qCT showed leptin group healed faster than control group, formulation of callus appeared early than that of control group, and the BMD analysis results showed that the BMD of tibias in LEP group were significantly different with NS group. Conclusion: ICV leptin could accelerate bone fracture healing at the bone defection model

Key words: ICV leptin, limbs fracture, bone fracture healing, Bone Morphogenetic Protein 2