Abstract: Study of the Effect of Stroner Neo-MinophagenC and Dexameth on the Biological Properties of Dermal Papilla Cells 【Abstract】Objective To observe the effects of Stroner Neo-MinophagenC (SNMC) and dexameth on the activity of dermal papilla cells (DPCs). Methods Dermal papillas were isolated from human scalp hair follicles by digestion with dispase and collagenase D and cultured. α-smooth muscle actin (α-SMA) was detected by immunohistochemical staining to identify DPCs. Effect of different concentration of SNMC and dexameth (0.5～100ng/ml) on the cell proliferation of DPCs were measured by MTT. Results Human dermal papillas were isolated obtained by "two-step enzymatic digestion". α-SMA was expressed in DPCs by immunohistochemistry method. The results of MTT showed that, 10,20,50μg/ml of SNMC could significantly promote the proliferation of DPCs (P <0.05), the effect of the proliferation was dose-dependent within concentration of 0.5～20μg/ml. 2.5μg/ml of dexameth could significantly promote the proliferation of DPCs (P <0.05), the effect of the proliferation was dose-dependent within concentration of 0.5～2.5μg/ml. Conclusion The mechanism of SNMC and dexameth may also induce hair growth by regulating the activity of DPCs in addition to anti-inflammator，immune regulation and suppression.

Key words: Stroner Neo-MinophagenC, dexamethasone, ermal papilla cells, cell culture, cell proliferation