Abstract: Abstract Objectives ：To evaluate the factor of signal pathway-protein kinase-C(pkc) in protective effect of one MAC sevoflurane post-conditioning on rat’s myocardial ischemia/reperfusion injury in Vivo. Methods：Rats model of Myocardial ischemia-reperfusion Injury were randomly divided into 4 groups：groupⅠsham operation (S); groupⅡischemia/reperfusion injury (I/R); groupⅢone MAC sevoflurane post-conditioning (Sevo); groupⅣSevo +Chelerythrine（Sevo+C）. The activity of CK-MB、SOD、MPO and concentration of Calcium ion were measured at the end of reperfusion. Myocardial infarction size was determined by dyeing with hematoxylin eosin (HE) and triphenyltetrazolium staining at the end of reperfusion. Results ：As far as the activity of CK-MB、SOD、MPO and concentration of Calcium ion, there are great significant differences among four groups at the end of reperfusion（P<0.05）. The concentration of Calcium ion in groupⅡ,groupⅢand groupⅣ are higher than groupⅠ（P<0.05）. Compared with groupⅡ, infarction size in groupⅢ、 groupⅣreduce significantly（P<0.05）. There is also great significant difference of infarction size between groupⅢand groupⅣ（P<0.01）.Conclusion: One MAC sevoflurane post-conditioning can attenuate ischemia/reperfusion injury through the activation of PKC signal pathway.

Key words: Sevoflurane postcondtioning, ischemia/reperfusion injury, PKC