Abstract: Abstract：Objective To investigate the activity of E2F-1 transcriptional factor in gastrointestinal cancer cells and its effect on cell cycle. Methods The expression of E2F-1 factor in 8 gastrointestinal cancer cell lines was detected immunohistochemically, and the cell cycle of cancer cells was analyzed by flow cytometry. Results The gastrointestinal cancer cells were divided into two groups, the E2F-1 high-expression group including HGC-27, BGC-823, SGC-7901, HT29, the E2F-1 low-expression group including MKN45, MKN28, SH288, SW-620. The ratios of G1/G0 phase, S phase and apoptosis in high-expression group were 33.48±4.61%, 63.91±5.02%, 1.50±0.56%, and in low-expression group were 58.58±3.41%, 35.38±4.03%, 5.61±1.44%. There were differences between these two groups. Conclusion The status of E2F-1 expression in cancer cells has a great effect on cell cycle. The E2F-1-positive cancer cells have a potency to grow with high rate.

Key words: Gastrointestinal cancer, Transcriptional factor, Cell cycle, Immunohistochemistry, Flow cytometry