Abstract: Abstract： Objective To investigate the association of tumor necrosis factor (TNF)-α, heat shock protein (HSP)70-2 gene polymorphisms and susceptibility of acute pancreatitis(AP). Methods Using case-control method， The gene polymor⁃ phism of TNF- α and HSP70- 2 was detected by PCR- RLFP in 72 patients with AP and 71 healthy controls. Results There were no significant differences in proportion of TNF-α genotype and alleles between AP and control groups (P > 0.05). There were no significant differences in TNF-α genotype and alleles between severe acute pancreatitis (SAP) and light acute pancreatitis (MAP) of AP group (P > 0.05). There were no significant differences in white blood cell count, C-reactive pro⁃ tein (CRP), amylase, three acyl glycerin and glucose between TNF-a and HSP70-2 gene of AA type and GA+GG type pa⁃ tients (P >0.05). The HSP70-2 genotype GA+GG proportion was significantly higher in AP group than that of control group （69.4% vs 49.3%). The ratio of patients with G allele was significantly higher in AP group than that of control group（46.5% vs 31.7%). The ratio of patients with GA + GG type AP was significantly higher in SAP patients than that of MAP patients of AP group（81.0% vs 53.3%). There was no significant difference in G allele between SAP and MAP patients (P＞ 0.05). Conclusion TNF-α polymorphisms is not associated with acute pancreatitis. There is an association between HSP70-2 polymorphisms and acute pancreatitis. Carrying the G allele increases the possibility of a severe acute pancreatitis， which is one of the genetic susceptibility factors of severe acute pancreatitis.　　　

Key words: tumor necrosis factor-alpha； HSP70 heat-shock proteins； pancreatitis； genetic polymorphism； HSP70-2； polymorphism,restriction fragment length, polymerase chain reaction