Abstract: Abstract： Objective To explore the clinical value of examining HPV E6 / E7 mRNA level in assessing cervical le⁃ sions infected with high- risk human papillomavirus (HR-HPV). Methods The cervical epithelial cells were collected from 265 patients with HR-HPV infection, including 100 cases of neoplasia free/ inflammation group (control group), 88 cas⁃ es of cervical intraepithelial neoplasia (CIN)Ⅰ, 33 cases of CINⅡ, 28 cases of CINⅢ and 16 cases of cervical carcinoma and the transcription of HPV E6 / E7 mRNA level was examined using branched DNA (b - DNA) technology. Results The positive rate HPV E6/E7 mRNA were higher in CIN Ⅱ (81.82%), CIN Ⅲ(89.29%) and cervical cancer group (100.00%) than tthat in control group (20.00%) and CINⅠ(35.23%) with significant difference, and there were no significant differences between other groups ; The positive rate and transcription level of HPV E6/E7 mRNA in HSIL (high grade squamous intraepi⁃ thelial lesion)and cancer group were significantly higher than normal, ASC(atypical squamous cell carcinoma) and LSIL（low grade squamous intraepithelial lesion) group (P < 0.05). Conclusion The transcription level of HPV E6 / E7 mRNA may re⁃ flect the activity of the virus and the progression of disease, and could be use as an effective indicator to screen high grade cervical pathological changes and a complementary method of cervical lesion screening.

Key words: cervical intraepithelial neoplasia； uterine cervical neoplasms, high-risk human papilloma virus, HPV E6/ E7 mRNA, branched DNA technologyvirus, HPV E6/E7 mRNA, branched DNA technology