Abstract: Objective To investigate the frequencies of genotype and allele distribution of IL12A gene single nucleotide polymorphisms rs568408and IL12B gene single nucleotide polymorphisms rs3212227 in Zhuang populations in Guangxi, and to compare the distribution of IL12A and IL12B polymorphisms among different races. Methods The IL12A gene rs568408 and IL12B rs3212227 polymorphisms were detected by SNaPshot SNP genotyping technique in 165 Zhuang populations in Guangxi, frequencies of genotype and allele of IL12A gene rs568408 and IL12B rs3212227 polymorphisms were analyzed in Zhuang populations compared with the other four populations (HapMap-HCB, HapMap-JPT, HapMap-YRI, HapMap-TSI) from HapMap database. Results There were IL12A and IL12B gene polymorphysms in Zhuang populations in Guangxi. The frequencies of allele and genotype distribution of IL12A gene rs568408 polymorphisms were not significantly differenct compared with HapMap-HCB, HapMap-JPT and HapMap-TSI (P>0.05), but were significantly different compared with HapMap-YRI (P<0.01); The frequencies of allele and genotype distribution of IL12B rs3212227 polymorphisms were not significantly differenct compared with HapMap-HCB and HapMap-JPT (P>0.05), but were significantly different compared with HapMap-YRI and HapMap-TSI (P<0.01) .Conclusion There are significant differences in the frequencies of allele and genotype distribution of IL12A gene rs568408 and IL12B gene rs3212227 between Zhuang populations and other ethnic populations, and this variation might contribute for a variety of clinical manifestation and morbidity of some IL-12 related diseases.