Abstract: Objective To investigate the expressions of HBXIP and GRIM-19 in hepatocellular carcinoma tissues and their clinic significance. Methods Hepatocellular carcinoma tissue (n=42) and normal liver tissue (n=28) were collected from Tianjin First Central Hospital，immunohistochemistry was used to detect the expressions of HBXIP and GRIM-19 in these two groups. Results Rate of cells with positive expressions of HBXIP in hepatocellular carcinoma and normal liver tissues were 80.95%（34/42）and 42.86%（12/28）respectively; Rate of cells with positive expression of GRIM-19 in hepato⁃ cellular carcinoma tissues and normal liver tissues was 40.48%（17/42）and 75.00%（21/28）respectively, and the difference between these two groups was statistically significant（P < 0.05）; The expression of HBXIP was higher but the expression of GRIM-19 was lower in poor differentiated and stage Ⅲ-IV cells than those in well and moderate differentiated cells and in stage I-II, cells. What′s more, the expression of GRIM-19 is higher in tissue without portal thrombosis than that in tissue with portal thrombosis. The expression of HBXIP was negatively correlated with GRIM-19 expression（rS=-0.400，P < 0.01）. Conclusion The abnormal expressions of HBXIP and GRIM-19 may play important roles in the process of development and metastasis of hepatocellular carcinoma.

Key words: carcinoma, hepatocellular, neoplasm metastasis, immunohistochemistry, HBXIP, GRIM-19