Table of Content

15 December 2015, Volume 43 Issue 12 Previous Issue    Next Issue
---
Cell and Molecular Biology
Experimental Study
Clinical Study
Applied Essay

Drug Clinical Evaluations
Review


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

Cell and Molecular Biology

Select

Effect of Asperosaponin Ⅵ on adipocyte differentiation in ST-2 cells and its underlying mechanisms WANG Haixiao，CUI Zhuang，WANG Baoli，BIAN Yuhong，ZHENG Fang 2015, 43 (12):  1345-1348.  doi: 10.11958/j.issn.0253-9896.2015.12.001 Abstract ( 805 )   PDF (339KB) ( 4027 )   Objective The effect of Asperosaponin Ⅵ（ASAⅥ）on adipocyte differentiation and the involvement of Wnt signal pathway was investigated. Methods The murine bone marrow stromal cell line ST- 2 were divided into 6 groups: control group, adipocyte differentiation group, and 4 different doses of ASAⅥ groups. Control group was exposed to the vehicle, adipocyte differentiation group was exposed to adipogenic reagent, and those 4 ASAⅥ groups were treated with different concentration（10-7, 10-6, 10-5, 10-4 mol/L）of ASAⅥ after adipocyte differentiation induction. 5 days later, oil red O staining was performed to calculate adipocyte rate. Then mRNA transcription levels of PPARγ, FABP4 genes and β-catenin that were Wnt/β-catenin signaling pathway proteins were examined by FQ-PCR. Then Wnt pathway inhibitor DKK1 was supplemented into ST-2 cells treated with 10-4 mol/L ASAⅥ for 5 days. After that FQ-PCR was used to detect whether tran⁃ scription levels of PPARγ, FABP4 and β-catenin in ST-2 cells were changed. Results Compared with adipocyte differenti⁃ ation group 10-5 mol/L and 10-4 mol/L ASAⅥ treatments greatly down-regulated the number of lipid droplets and markedly inhibited transcription levels of adipocyte characterization transcription factors included PPARγ, FABP4 while up-regulat⁃ ed transcription level of β-catenin in ST-2 cells. DKK1 can reverse the inhibitory effect of ASAⅥ on adipocyte differentia⁃ tion in ST-2 adipocyte. The transcription levels of PPARγ and FABP4 were up-regulated significantly while transcription level of β-catenin was inhibited. Conclusion ASAⅥ blocks adipocyte differentiation in ST-2 cells which might be medi⁃ ated through activating Wnt/β -catenin signaling pathway. Related Articles | Metrics

Select

Association between ADAM33 gene polymorphism with chronic obstructive pulmonary disease incidence in Kazakh of Xinjiang WANG Shasha, GUAN Jian△, WANG Shan, CHENG Fangjuan, REN Xia, XU Xilin, GAO Yan 2015, 43 (12):  1349-1353.  doi: 10.11958/j.issn.0253-9896.2015.12.002 Abstract ( 734 )   PDF (460KB) ( 3951 )   Objective To explore correlation of Xinjiang Kazakh population who suffered from COPD with polymor⁃ phisms of F+1,S2,T1,ST+5 locus of ADAM33 gene. Methods Blood samples (n=193) from healthy controls (Control group, n=193) and COPD patients (Case group, n=197) were detected by SNP SNaP shot. Results Comparing case group with the control group, gene frequency and allele frequency of F+1 locus were of significant differences (P < 0.05). In patient group, there were no significant differences in F+1 locus genotype and in clinical indicators include lung function FEV1 predicted and FEV1/FVC (P > 0.05). The gene frequencies and allele frequency of S2、T1 and ST+5 locus were not significantly differ⁃ ent between case group and control group (P > 0.05). F+1 and S2 locus were analyzed by haplotype analysis which showed that there was significant differences in Hap1 (CC) haplotype between case group and control group (P < 0.05), and OR < 1 indicated that its haplotype may reduce the risk of COPD . There were significant differences (P < 0.05) in Hap3(TC) haplo⁃ type between case group and control group and OR > 1 revealed that its haplotype may increase the risk of COPD . The distri⁃ bution of Hap2 (TG) and Hap4 (CG) were not significantly different (P > 0.05) between the 2 groups. T1 and ST+5 locus were analyzed by haplotype analysis which showed significant differences in haplotypes between case group and control group (P < 0.05). Conclusion The occurrence of COPD may be related to the polymorphism of ADAM33 gene in F+1 locus in Xinjiang Kazakh. Related Articles | Metrics

Select

The impact of miR-320-3p on adipocyte differentiation WANG Changlan, GAO Zhihong, CHANG Ailing, WANG Baoli 2015, 43 (12):  1353-1355.  doi: 10.11958/j.issn.0253-9896.2015.12.003 Abstract ( 901 )   PDF (595KB) ( 4634 )   Objective To study the role of miR-320-3p in adipocyte differentiation. Methods Marrow mesenchymal stem cells were isolated from mice and cultured then induced with adipogenic agents for 3 days. The transcription level of miR-320-3p was examined by qRT-PCR. Stromal ST2 cells were transfected with miR-320-3p, followed by adipogenic treatment. Oil-red O staining and qRT-PCR were employed to assess the differentiation of adipocytes induced by miR-320- 3p. Results The expression level of miR-320-3p increased in MSCs after adipogenic treatment (P < 0.01). Addition of miR-320-3p in ST2 cells promoted the formation of oil-red O positive adipocytes and up-regulated the expression levels of adipogenic transcription factors such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα) and the marker gene adipocyte fatty acid binding protein 4 (FABP4)，compared to cells that transfected with miR-320-3p mimics (P < 0.05). Conclusion miR-320-3p promotes ST2 cells differentiation into adipocytes. Related Articles | Metrics

Select

Effects of ZHX3 gene silence on the expression of osteoblast-related factors in BMSCs ZHANG Miaomiao, BAO Cuifen, WANG Yan, MIN Heming, QIN Shujian 2015, 43 (12):  1356-1360.  doi: 10.11958/j.issn.0253-9896.2015.12.004 Abstract ( 599 )   PDF (899KB) ( 3927 )   Objective To investigate the effects of zinc fingers and homeoboxes 3 (ZHX3) silence on expressions of smad3, smad4 and RUNX2 in bone marrow mesenchymal stem cells (BMSCs). Methods ZHX3 low expression vector (ZHX3 silent group) was constructed and was transfected to rat BMSCs. Empty vector was transfected into BMSCs and was used as vehicle control group, and wild type BMSCs was used as the control group. The cell transfection rate was measured under a fluorescence microscope, and then the successful transfection was identified. The immunocytochemistry and immu⁃ noblotting methods were used to detect the expression levels of smad3, smad4 and RUNX2. Results (1) Cells with BMSCs phenotype can be obtained by recovery culturing. (2) After transfection, the green fluorescent protein was found in ZHX3 si⁃ lence group and vehicle control group. Blank control group showed no significant fluorescence. The expression level of ZHX3 was significantly lower in ZHX3 silence group than that of vehicle control group. (3) Results of immunofluorescence asssay showed that the positive expressions of smad3 and smad4 were located in nucleus and cytoplasm, the positive expression of RUNX2 was mainly located in nucleus. Positive cells were observed in three groups. There was no significant difference in fluorescence intensity between the control group and the vehicle control group, but the fluorescence intensity was significant⁃ ly lower in ZHX3 gene silence group than that of two control groups. (4) There were no significant differences in expressions of smad3, smad4 and RUNX2 betweem control group and the vehicle control group, but they were significantly higher than those of ZHX3 silence group（P < 0.05）. Conclusion ZHX3 gene silence can delay vitro osteogenesis of BMSCs, which may play a role by the down-regulated expression levels of smad3, smad4 and RUNX2. Related Articles | Metrics

Select

Effects of glycyrrhizin on the expressions of IL-2 and IL-4 in HaCaT cells YIN Cuihong, CHE Yamin 2015, 43 (12):  1361-1364.  doi: 10.11958/j.issn.0253-9896.2015.12.005 Abstract ( 666 )   PDF (321KB) ( 4129 )   Objective To observe the effect of glycyrrhizin (CG) on the proliferation and the expressions of interleukin (IL)-2, IL-4 in human keratinocytes cell line HaCaT cells, and to investigate the therapeutic mechanism of CG in the pa⁃ tients with psoriasis. Methods The HaCaT cells were divided into four groups: blank group (without CG), low concentration group (50 μL CG/mL cell supernatant), medium concentration group (100 μL CG/mL cell supernatant) and high concentra⁃ tion group (200 μL CG/mL cell supernatant). Methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the prolifer⁃ ation of HaCaT cells that were treated with CG at different concentrations respectively for 24 hours. The contents of IL-2 and IL-4 in HaCaT cells were examined by enzyme linked immunosorbent assay (ELISA). Results Compared with the blank group, there were obviously inhibitory effects in the proliferation of HaCaT cells treated by low，medium and high concentra⁃ tions of CG for 24 hours. There was no significant difference in IL-2 level between low concentration CG group and blank group (ng/L：234.51±17.98 vs 225.31±16.23, P > 0.05). After 24 hours of intervention with CG of medium and high concen⁃ trations, the expression levels of IL-2 was significantly lower in HaCaT cells than that in blank group and low concentration group. The IL-2 level was significantly lower in high concentration group than that in medium concentration group (ng/L: 188.99±19.22 vs 208.49±18.40, P＜0.05). At the same condition, the secretion of IL-4 in HaCaT cells was significantly upregulated in high concentration group than that in other three groups (ng/L: 45.67±10.29 vs 37.62±3.90, 39.68±6.08, 43.85± 10.26, P＜0.05). Conclusion Glycyrrhizin may suppress the proliferation of human skin keratinocytes by regulating secre⁃ tion of cytokines IL-2 and IL-4. Related Articles | Metrics

Select

Expressions of HBXIP and GRIM-19 in hepatocellular carcinoma and their clinic significance SUN Jisan, ZHANG Jianjun, GAO Wei, YANG Tao, GUO Qingjun, MA Nan, WANG Honghai, JIANG Wentao 2015, 43 (12):  1364-1367.  doi: 10.11958/j.issn.0253-9896.2015.12.006 Abstract ( 717 )   PDF (521KB) ( 3874 )   Objective To investigate the expressions of HBXIP and GRIM-19 in hepatocellular carcinoma tissues and their clinic significance. Methods Hepatocellular carcinoma tissue (n=42) and normal liver tissue (n=28) were collected from Tianjin First Central Hospital，immunohistochemistry was used to detect the expressions of HBXIP and GRIM-19 in these two groups. Results Rate of cells with positive expressions of HBXIP in hepatocellular carcinoma and normal liver tissues were 80.95%（34/42）and 42.86%（12/28）respectively; Rate of cells with positive expression of GRIM-19 in hepato⁃ cellular carcinoma tissues and normal liver tissues was 40.48%（17/42）and 75.00%（21/28）respectively, and the difference between these two groups was statistically significant（P < 0.05）; The expression of HBXIP was higher but the expression of GRIM-19 was lower in poor differentiated and stage Ⅲ-IV cells than those in well and moderate differentiated cells and in stage I-II, cells. What′s more, the expression of GRIM-19 is higher in tissue without portal thrombosis than that in tissue with portal thrombosis. The expression of HBXIP was negatively correlated with GRIM-19 expression（rS=-0.400，P < 0.01）. Conclusion The abnormal expressions of HBXIP and GRIM-19 may play important roles in the process of development and metastasis of hepatocellular carcinoma. Related Articles | Metrics

Select

Role of TNF-α in promoting migration and invasion of colon cancer cells ZHAO Peng, CHEN Junmao, CAO Wenbin, YANG Guanghua, YU Xiangyang, LIU Chunhui, ZHENG Yang 2015, 43 (12):  1368-1372.  doi: 10.11958/j.issn.0253-9896.2015.12.007 Abstract ( 676 )   PDF (910KB) ( 4165 )   Objective To explore the effect of TNF-α on expression of TROP-2 and to explore the role of TROP-2 in the metastasis and invasion of colon cancer HCT-116 cells. Methods HCT-116 cells were cultured and treated with 0, 10, 20, 30, 50, 100 and 200 μg/L TNF-α. Cell viability was assessed by MTT. The expression of TROP-2 was determined by western blot. The effects of 20 μg/L TNF-α on cell migration and invasion were investigated by wound healing assay and Transwell method. Small interfering RNA (siRNA) was used to knock down endogenous TROP-2 expression. The transcrip⁃ tion and translation levels of TROP-2 were detected by qPCR and Western blot respectively. The migratory and invasive ca⁃ pability of HCT-116 cells transfected with TROP-2 siRNA were checked by wound healing assay and Transwell method re⁃ spectively. Results There is no significant change of cell viability between HCT-116 cells treated with 0,10, 20, 30 and 50 μg/L TNF-α, but cell viability of HCT-116 decreased significantly with treatment of 100 μg/L and 200 μg/L TNF-α. Low concentration of TNF-α (≤50 μg/L) led to increase of TROP-2 protein expression that peaks when 20 μg/L TNF-α was add⁃ ed. High concentration of TNF-α (100, 200 μg/L) result in decrease of TROP-2 protein. TROP-2 siRNA significantly downregulated the expression of TROP-2 at both mRNA and protein levels in colon cancer HCT-116 cells. Compared with con⁃ trol group, silencing TROP-2 by TROP-2 siRNA inhibited the migratory and invasive capability of HCT-116 cells. Wound healing rate and the number of transwell cell both decreased in siRNA group compared with those of control group (P < 0.05). Conclusion The mechanism that low concentration of TNF-α promoted HCT-116 cells migration and invasion might be through up-regulating the expression of TROP-2. Related Articles | Metrics

Experimental Study

Select

Effect of Exendin-4 on oxidative stress and neural apoptosis following spinal cord injury ZHAO Xingzhang, LI Haotian, WANG Jiquan, SUN Ping, CHU Xin, LYU Gang, FAN Zhongkai 2015, 43 (12):  1373-1376.  doi: 10.11958/j.issn.0253-9896.2015.12.008 Abstract ( 766 )   PDF (418KB) ( 4266 )   Abstract: Objective To study the effect of Exendin-4 on oxidative stress and neural apoptosis following spinal cord injury (SCI). Methods Adult male SD rats, with weight between 200-250 g, were randomly divided into three groups (12 in each group): Sham group, SCI group and Exendin-4 group (Ex-4 group). Rats in Sham group achieved spinal cord exposure. SCI group and Ex-4 group were induced according to Allen′s test (using a weight-drop device). Rats in Ex-4 group were ad⁃ ministrated with Exendin-4 (10 μg/rat) through intraperitoneal injection immediately after establishment of SCI models. Rats in Sham group and SCI group were given the same volume of normal saline solution instead. Level of malondialdehyde (MDA) and the activity of catalase (CAT) were assessed in spinal cord tissues 24 hour after drug administrations. Neural apoptosis was detected by TUNEL staining and the expression levels of caspase-9 and AIF were determined using Western blot. Results Compared with Sham group, the levels of MDA, caspase-9 and AIF as well as neuronal apoptosis rate in⁃ creased obviously, while activity of CAT decreased markedly in SCI group（P < 0.01）. Compared with SCI group, the levels of MDA, caspase-9 and AIF as well as the neuronal apoptosis rate decreased obviously, while activity of CAT increased re⁃ markably in Exendin-4 group（P < 0.01）. Conclusion Exendin-4 restrain neural apoptosis following spinal cord injury through relieving oxidative damage. Related Articles | Metrics

Select

Comparison of different ways to trace BMSCs after cerebral ischemia-reperfusion injury WANG Yan, MIN Heming, ZHANG Miaomiao, BAO Cuifen, MIN Lianqiu 2015, 43 (12):  1377-1381.  doi: 10.11958/j.issn.0253-9896.2015.12.009 Abstract ( 966 )   PDF (1927KB) ( 3907 )   Objective To compare different ways to trace bone marrow mesenchymal stem cells (BMSCs) after being transplanted in cerebral ischemia-reperfusion injury. Methods Male SD rats of SPF grade were randomly divided into sham group, model group (ischemia-reperfusion，IR), BrdU tracing group, PKH26 tracing group and GFP tracing group. Fo⁃ cal cerebral ischemia-reperfusion model was established by blocking middle cerebral artery. 24 hours after cerebral isch⁃ emia-reperfusion injury, 10 μL BMSCs that were labeled respectively by BrdU, PKH26, GFP were added respectively into BrdU, PKH26 and GFP tracing group while equal volum of normal saline was added into sham group and model group. Mod⁃ el and transplanting cells efficacy was determined by neural behavioral score, TTC staining and brain water content; Neurons were counted using tar violet staining; The number of transplant cells in the transplanting site was assessed by fluorescence microscopy. Results Before transplanting, there was no significant difference among BrdU, PKH26 and GFP group in cell labeled efficacy. By contrast, neural behavioral score, brain infarct volume and brain tissue water content were significantly lower in all three tracing groups than that in model group 4 weeks after transplantation while neuron counts were markedly higher. There was no significant difference of above parameters among the three tracing groups. However, the number of traced transplanting cells in damaging area in GFP group is significantly higher than that in BrdU group and PKH26 group. Conclusion In cerebral ischemia-reperfusion injury, the tracing effect of GFP last longer, therefore it is significantly more effective than BrdU and PKH26. Related Articles | Metrics

Select

Protective effects of Trimetazidine on ischemia-reperfusion injury of fatty liver in rats WANG Tengfei，YANG Tao，ZHANG Wei，FANG Zhenyu，WANG Honghai，LIU Lei，ZHANG Haiming，WANG Yuliang 2015, 43 (12):  1382-1385.  doi: 10.11958/j.issn.0253-9896.2015.12.010 Abstract ( 825 )   PDF (705KB) ( 3902 )   Objective To investigate the protective effects of Trimetazidine（TMZ）on the ischemia reperfusion injury （IRI）of fatty liver in autotransplantation model. Methods Fatty liver model was established by feeding high fat diet. Male Wistar rats (n=30) were randomized into three groups; Sham group, TMZ group and Model group. Liver was autotransplanted in both TMZ group and Model group. Serum levels of ALT, SOD, MDA, Bcl-2 and activated Caspase-3 were assessed 6 hours after the operation. The pathological performances of liver were also determined. Results Compared with the Model group, serum levels of ALT,AST, MDA and SOD levels decreased significantly in the TMZ group（P < 0.05）. Serum level of Bcl-2 was higher while level of activated Caspase-3 was lower in TMZ group than those in Model group（P＜0.05）. Histo⁃ logical assay and TUNEL staining showed reduced hepatocyte swelling and narrowed sinusoid as well as decreased hepatic apoptosis in TMZ group compared with Model group. Conclusion TMZ can reduce oxidative stress, promote the expression of Bcl-2 and inhibit the activation of Caspase-3, which all contribute to its protective effect on fatty liver with ischemia-re⁃ perfusion injury. Related Articles | Metrics

Select

The effects of polysaccharides and pioglitazone on mouse model of pulmonary adenocarcinoma DENG Zenghua,XIE Hui, CHEN Zhengang, WANG Guangshun, ZHANG Chenggang 2015, 43 (12):  1386-1389.  doi: 10.11958/j.issn.0253-9896.2015.12.011 Abstract ( 548 )   PDF (2207KB) ( 3883 )   Objective To provide theoretical reference for clinical therapy of pulmonary adenocarcinoma by evaluating the effects of polysaccharides and pioglitazone on mouse model of pulmonary adenocarcinoma and to explore the relationship between inflammation and pulmonary adenocarcinoma. Methods One hundred mice were averagely divided into five groups, including control group, model group, polysaccharides group, pioglitazone group, polysaccharides and pioglitazone group (unite group). Polysaccharides solution (500 mg/kg) was given to polysaccharides group, pioglitazone solution (15 mg/kg) was given to pioglitazone group, polysaccharides solution (500 mg/kg) and pioglitazone solution (15 mg/kg) were given to unite group; and the equal volume of saline (10 mL/kg) was given to control and model group (1 t/d, 5 d/w, continuously 20 w ). The pulmonary adenocarcinoma induced by urethane was evaluated in each group at different time points. The levels of NF-κB, TNF-α, IL-1β and IL-6 were measured in each group at the 12th week and the 20th week respectively. Results The body weights were increased in the control group, which were decreased in other groups during urethane-injection, but increased continuously after the injection. At the 20th week, nodules were found in lung surfaces in all mice except mice of control group. The lung index was higher in all mice except mice of control group. The levels of NF-κB, TNF-α, IL-1β and IL-6 were significantly higher at 12th week and 20th in model group, polysaccharides group, pioglitazone group, polysaccha⁃ rides and pioglitazone group than those of control group. The levels of NF-κB, TNF-α, IL-1β and IL-6 were significantly lower in polysaccharides group, pioglitazone group, polysaccharides and pioglitazone group than those of model group. Con⁃ clusion Sustained inflammatory response is one of the risk factors for the development of lung adenocarcinoma. Polysaccha⁃ rides and pioglitazone can reduce the level of inflammation in mouse lung adenocarcinoma, suggesting that both of them can be used as potential adjuvant in the clinical treatment of lung adenocarcinoma. Related Articles | Metrics

Select

The detection of motor nerve injury by MUNE in an animal model of diabetes TIAN Li, CHENG Yan, ZHANG Zhecheng, LIU Na, ZHU Ju 2015, 43 (12):  1390-1393.  doi: 10.11958/j.issn.0253-9896.2015.12.012 Abstract ( 719 )   PDF (1057KB) ( 4548 )   Objective To investigate motor nerve function status in rats with diabetes mellitus by motor unit number estimation (MUNE), and discuss it′s early diagnostic value in diabetic peripheral neuropathy (DPN). Methods Diabetic rat model (DM group) was induced by streptozotocin. The MUNE of gastrocnemius muscle and motor nerve conduction (MCV, CMAP) of the sciatic nerve were measured at the 4th, 8th and 12th week after onset of hyperglycemia in the DM group and the control group (normal SD rats). The ultrastructure of sciatic nerve was observed by electron microscope. Results At the 4th week, MUNE of gastrocnemius muscle was significantly decreased in DM group compared to that of the control group (275.88±87.87 vs 369.71±75.64，P < 0.05). There were no significant differences in MCV and CMAP of sciatic nerve be⁃ tween two groups. The electron microscopy observation showed that most nerve fibers were normal; a small amount of axonal atrophy, and myelin lamellar structure was separated in DM group. At the 8th week, compared with the control group, MUNE were reduced in gastrocnemius muscle in DM group (357.49±72.68 vs 221.26±92.41, P < 0.01). There were no significant dif⁃ ferences in MCV and CMAP of the sciatic nerve between DM group and control group. The electron microscope observation showed that part of nerve fibers were normal, the myelin focal plate layer was loose and separated, axonal atrophy, the axonal membrane and myelin sheath inner layer was separated with big gap. At the 12th week, MUNE of gastrocnemius muscle (127.87±19.80 vs 366.85±51.25), sciatic nerve MCV [(35.06±4.43) m/s vs (50.47±6.07) m/s] and CMAP [(2.91±1.37) mV vs (5.98±2.14) mV] were significantly decreased in DM group than those of control group (P < 0.01). The electron microscopy observation showed severely damaged myelin flex and axonal squeeze. Conclusion MUNE is much earlier in detecting ear⁃ ly motor nerve dysfunction in DM than conventional motor nerve conduction test. Related Articles | Metrics

Select

Quantitative analysis of renal injury using CEUS in hypertensive rabbits model induced by L-NAME MENG Xianglu, ZHANG Guohui, WANG Wenhong, SHAO Hong, GUO Jun, ZHAO Xiaoxin, GU Cheng, ZHANG Xiaodan 2015, 43 (12):  1394-1397.  doi: 10.11958/j.issn.0253-9896.2015.12.013 Abstract ( 726 )   PDF (727KB) ( 4252 )   Objective To assess the value of contrast-enhanced ultrasound (CEUS) on quantitative analysis of re⁃ nal cortex perfusion in hypertensive rabbits model. Methods Hypertensive rabbit modal (n=10) were established by inject⁃ ing N-nitro-L-arginin methylester (L-NAME). CEUS and Cystatin C (CysC) serum level analysis were performed at differ⁃ ent time points: before and the 2nd, 4th, 6th and 8th week after injecting L-NAME. Time-intensity curve and area under curve (AUC) were analyzed quantatively while correlation of AUC and CysC were also analyzed. Results Serum level of Cys C in⁃ creased significantly at the 6th week after L-NAME administration which is earlier than the increase of serum levels of Scr and BUN. AUC decreased at first then increased after L-NAME administration. Upon addition of L-NAME, rise time (RT) and peak intensity (PI) decreased while mean transit time (MTT), time from peak to one half (HPT) and time to peak (TTP) in⁃ creased. Our study confirmed a positive correlation between AUC and Cys C (r=0.950, P < 0.001). Conclusion Setting up rabbits model by L-NAME is convenient and reproducible, which is an useful tool in experimental study of preclinical and clinical phase of hypertensive renal injury. CEUS combining with CysC serum level analysis is considered as an effective technology for evaluating renal function in hypertensive patients. Related Articles | Metrics

Select

Protective roles of Emodin in the intestinal mucosal layer of rats with severe acute pancreatitis CHEN Xia, ZHAO Hongxian, WANG Qiaozhi, LI Changping 2015, 43 (12):  1398-1400.  doi: 10.11958/j.issn.0253-9896.2015.12.014 Abstract ( 676 )   PDF (1207KB) ( 4040 )   Objective To explore the protective roles of Emodin in the intestinal mucosal lay of rats with severe acute pancreatitis (SAP) and its mechanism. Methods SD rats (n=30) were divided into 3 groups: sham operation group, SAP group and Emodin group (SAP rats treated with Emodin). The SAP rat models were established via retrograde injection of 3% sodium taurocholate to pancreatic duct. Rats in Emodin group were peritoneally injected with Emodin (2.5 mg/100 g) at both 1 hour and 3 hour after sodium taurocholate injection. Apoptosis of intestinal epithelial cell was detected by TUNEL analy⁃ sis. The expression of glucose-regulated protein78 (GRP78) protein was assessed by immunohistochemistry. Results Com⁃ pared with sham operation group, apoptosis in intestinal epithelial cells and the expression of GRP78 protein were increased significantly in SAP group（P＜0.05）. Emodin treatment reduced AP-induced mucosal intestinal epithelial cell apoptosis （P＜0.05）. But there is no significant difference of GRP78 expression between SAP group and Emodin group（P＞0.05）. Conclusion Emodin has a protective effect on intestinal layer in rats with SAP through inhibiting intestinal epithelial cell apoptosis. However, ER stress is not likely to be involved in this protective effect. Related Articles | Metrics

Select

The role of rapamycin-eluted stent in vascular endothelial injury YUAN Ling，NIE Wei，GAO Ping，CHEN Bin，LIU Weiwei，CUI Xiaoxue 2015, 43 (12):  1401-1404.  doi: 10.11958/j.issn.0253-9896.2015.12.015 Abstract ( 670 )   PDF (636KB) ( 3939 )   Objective To explore the mechanism of rapamycin -eluted stent in vascular endothelial injury. Meth⁃ ods （1）Rapamycin (rapamycin group) was injected to rabbit dorsum muscle to simulate rapamycin-eluted stent implanta⁃ tion into muscles. Control group and acetone control group were established at the same time. Morphological change in mus⁃ cle was observed and serum calcium levels were measured after rapamycin injection.（2）HUVECs were incubated with 0.1, 1, 10 and 100 μg/L rapamycin for 48 h respectively or with 1 μg/L rapamycin for 6, 12, 24 and 48 h respectively．Cell via⁃ bility was examined by MTT and its relationship with drug concentration and treatment time were analyzed.（3）HUVECs were divided into control group and 1 μg/L rapamycin group. After 48 h，morphological changes of HUVECs were assessed by HE staining，the production of nitric oxide was examined by Nitric Oxide Assay Kit and the intracellular calcium ion con⁃ centration was tagged with Fluo-3/AM. Results (1) Organizational morphology in local muscle with rapamycin injection represent stent implantation of rabbit，and calcium content in local muscle increased significantly in rapamycin group com⁃ pared with nomal control group and acetone control group（P < 0.05）. (2) Cell survival rate decreased significantly upon ad⁃ ministration of rapamycin in both concentration and time dependent manner（P < 0.01）. (3) In rapamycin group, cytoplasm vacuoles, nucleus pycnosis and nuclear fragmentation were observed；compared with control group，the levels of intracellular free Ca2+ increased while the levels of nitric oxide was reduced. Conclusion Rapamycin treatment lead of injury to vascular endothelial cells which might through up-regulating intracellular Ca2+ level. Related Articles | Metrics

Clinical Study

Select

Correlation between plasma homocysteine level and ventricular remodeling in elderly patients with essential hypertension HUANG Jiaohong, WANG Lin, LIU Bainian, YANG Limin, GUO Xiaokun, ZHANG Hongmei, WANG Chao 2015, 43 (12):  1405-1408.  doi: 10.11958/j.issn.0253-9896.2015.12.016 Abstract ( 714 )   PDF (343KB) ( 3829 )   Objective To explore the correlation of serum homocysteine（Hcy）levels and hypertrophy of left ventricle in very elderly hypertensive patients．Methods According to plasma Hcy levels，patients with essential hypertension (n= 378) were divided into non H-type hypertension group (n=142) and H-type hypertension group (n=236). Height, weight, reg⁃ ular medication, blood pressure, renal function, blood lipid profile and the concentration of plasma Hcy were recorded. Color Doppler ultrasonic equipment was used to determine the morphology and structure of left ventricle. The correlation between plasma Hcy and left ventricle remodeling was analyzed. Results The ratio of left ventricular hypertrophy was higher in Htype hypertension group than that in non H-type hypertension group（45.8% vs 24.6%，χ2=16.81，P < 0.001）. Patients in Htype hypertension group had higher systolic blood pressure, higher plasma level of Hcy and larger left ventricular posterior wall thickness（LVPWT), larger interventricular septal thickness (IVST) and increased left ventricular mass index (LVMI) compared to those in non H-type hypertension group（162.20±14.97）vs（149.70±5.06）mmHg，（19.76±5.83）μmol/L vs （9.53±0.72）μmol/L，（9.77±2.35）vs（9.21±2.68）mm,（9.74±3.15）vs（8.51±2.42）mm,（118.64±39.38）vs（101.85±41.71）g/m2 respectively, all P＜0.05）. There was a positive correlation between LVMI and Hcy（r=0.381，P < 0.001）. Multivariable Lo⁃ gistic regression analysis showed that hyperhomocysteinemia was an independent risk factor of LVMI. Conclusion High plasma Hcy level is an independent risk factor of LVMI, which works together with hypertension to promote left ventricular re⁃ molding. Related Articles | Metrics

Select

CDK5 and epithelial-mesenchymal transition related proteins are abnormally expressed in head and neck squamous cell carcinoma ZHAO Minghui, HUANG Yuanyuan, SUN Shanshan, KONG Lingping, WANG Yu, GUO Wenyu, ZHOU Xuan, WANG Xudong, ZHANG Lun 2015, 43 (12):  1408-1412.  doi: 10.11958/j.issn.0253-9896.2015.12.017 Abstract ( 871 )   PDF (712KB) ( 4211 )   Objective To explore the expressions of Cyclin-dependent kinase 5 (CDK5) and Epithelial-Mesenchymal Transition (EMT) related proteins including N-cadherin, Vimentin and E-cadherin in head and neck squamous cell carcinoma (HNSCC), and to determine the relationship between the expression of CDK5 and prognosis. Methods The expression levels of CDK5 and EMT related proteins were evaluated by immunohistochemistry in 55 patients who were diagnosed as HN⁃ SCC. They were also analyzed in different clinical pathological factors. The correlation of CDK5 and EMT related proteins as well as the relationship between the expression of CDK5 and prognosis were also analyzed. Results The expression level of CDK5 was significantly higher in patients with lymph node metastasis than that in patients with non-lymph node metastasis (91.67% vs 30.23%, P < 0.05). It’s also higher in T3-T4 stages than that in T1-T2 stages (85% vs 20%, P < 0.05). The ex⁃ pression levels of N-cadherin and Vimentin were significantly higher in patients with lymph node metastasis than those in patients with non-lymph node metastasis (75.00% vs 6.98%; 91.67% vs 27.91%, all P < 0.05). However, the expression level of E-cadherin was significantly lower in patients with lymph node metastasis (8.33% vs 86.05%, P < 0.05) compared to that in patients without. CDK5 was positively correlated with N-cadherin and Vimentin, but negatively correlated with E-cad⁃ herin (rs=0.512, 0.443, -0.363, all P < 0.01). The 3-year survival rates were significantly lower in patients with high expres⁃ sion of CDK5 (37.5%) than that in patients with low expression of CDK5 (87%, Log-rank χ2=12.678, P < 0.01). Conclusion CDK5 and EMT related proteins were activated abnormally in HNSCC with lymph node metastasis. CDK5 may be a new bio⁃ logical marker for prognosis of HNSCC. Related Articles | Metrics

Select

The expression of OCT4 and P27kip1 in renal hyaline cell carcinoma and their correlations GUAN Youliang, ZANG Dongyu, WANG Dan 2015, 43 (12):  1413-1416.  doi: 10.11958/j.issn.0253-9896.2015.12.018 Abstract ( 589 )   PDF (378KB) ( 3818 )   Objective To investigate the expressions of OCT4 and P27kip1 and their correlations in renal hyaline cell carcinoma. Methods A total of 24 samples of renal hyaline cell carcinoma were detected by immunohistochemistry. The rate of cells with positive labelling of OCT4 and P27kip1 and their intra cellular distribution were observed in renal hyaline cell carcinoma. The expression levels of OCT4 and P27kip1 were compared between different gender, age (＜60y and ≥60y) and cancer cell metastasis groups. Results The rates of cells with positive OCT4 and P27kip1 expressions were 66.7% and 75% in renal hyaline cell carcinoma respectively. The ratio of cells with high, middle and low expression of OCT4 were 33.3%, 20.5% and 12.5% in renal hyaline cell carcinoma tissues. OCT4 was mainly expressed in cytoplasm and nuclei with a few was expressed in the cell membrane. The ratio of cells with high, middle and low expression of P27kip1 were 12.5%, 25% and 37.5% in renal hyaline cell carcinoma tissues respectively. The positive staining of P27kip1 was in the cytoplasm and cell membrane, and a small number of nuclei were expressed. There were no significant differences in OCT4 and P27kip1 between different age and gender groups. There were significant differences in OCT4 and P27kip1 expressions between patients with metastasis and patients without metastasis (P < 0.05). A negative correlation was found between OCT4 and P27kip1 expres⁃ sions in renal hyaline cell carcinoma (rs=-0.408, P < 0.05). Conclusion The expression of OCT4 is negatively correlated with the expression of P27kip1. Inhibiting expressions of OCT4 or P27kip1, especially knocking down P27kip1 in cytoplasm can be used as a new diagnosis and treatment of renal cancer gene therapy. Related Articles | Metrics

Select

Correlation of serum vitamin D level with pulmonary function related factors in adult asthma ZENG Fanrui，ZHANG Linghui，SHA Hang，Liu Yongmou，WU Juan 2015, 43 (12):  1416-1419.  doi: 10.11958/j.issn.0253-9896.2015.12.019 Abstract ( 799 )   PDF (312KB) ( 4882 )   Objectives To explore the correlation of level of serum 25-hydroxyvitamin D with pulmonary function in adult with asthma. Methods Patients were divided into Asthmatic group（n=62）and Control group (n=28). The Asthmatic group was further divided into Mild Group (n=6), Moderate Group (n=13) and Severe Group (n=43). Serum levels of 25- hy⁃ droxyvitamin D [25(OH)D], denoted as "25(OH) Vit D" was detected by ELISA. Pulmonary function indicators，including FVC (forced vital capacity) , FEV1 (forced expiratory volume in 1 second) , FEV1% predicted , and FEV1/FVC% were deter⁃ mined by a pulmonary function testing device. General profiles such as medical history, age and height as well as serum VitD levels were compared between subgroups of the asthmatic groups and between two genders. Serum levels of 25 (OH) VitD were compared between asthmatic group and control group while its correlation with FEV1% predicted were calculated in all three sub asthmatic groups. Results There was no significant difference in medical history, age, height and the 25(OH) VitD levels between male and female participants. Serum 25(OH) Vit D level was significantly lower in the asthmatic patient group [(29.69±20.45) nmol/L] compared to that in control group [(75.16±4.06) nmol/L] (P < 0.05). It was significantly lower in severe sub group than those in the mild and moderate sub groups. The differences were both statistically significant (P <0.05). There were positive correlations between serum 25(OH) Vit D levels and FEV1% predicted ( P < 0.05) in all sub asth⁃ matic groups. Conclusion Vitamin D deficiency is highly prevalent in asthmatic patients, and there is a strong correlation between 25(OH) Vit D asthma severity as well as between lung function. Related Articles | Metrics

Select

Clinical characteristics of 92 misdiagnosis cases of tracheobronchial tuberculosis and the clinic value of endoscope XUE Hui，XING Lihua，ZHANG Cailian，QIN Chao，ZHANG Dong，DU Zhongzhen 2015, 43 (12):  1420-1424.  doi: 10.11958/j.issn.0253-9896.2015.12.020 Abstract ( 875 )   PDF (1339KB) ( 4607 )   Objective To investigate the reasons of tracheobronchial tuberculosis misdiagnosis and its clinical charac⁃ teristics as well as the diagnostic value of bronchoscope. Methods Clinical data of 92 cases of misdiagnosis of tracheobron⁃ chial tuberculosis by electronic bronchoscopy in our department from January 2006 to January 2012 were analyzed retrospec⁃ tively. Bronchoscopy, endoscopic biopsy, brushing, lavage and radiological images were all compared. Results Clinical symptoms and laboratory tests showed no specificity in diagnostic value; Chest X-ray was not typical. Bronchial stenosis was seen in 45 cases（48.9%）and bronchial obstruction was seen in 6 cases（6.5%）as shown in chest CT while no abnormality in the bronchus was seen in 41 cases（44.6%）. Bronchoscopy revealed 28 cases (30.4%) of inflammatory infiltration, 14 cas⁃ es (15.2%) of necrotizing ulceration, 35 cases (38.0%) of granulation hyperplasia and 15 cases (16.3%) of Scar stricture. En⁃ doscopic biopsy confirmed 56 cases (60.9%), while bronchoscopic brushing and examination of acid-fast bacillus approved 32 cases (34.8%). Then, bronchoscopic lavage of acid-fast bacillus verified 39 cases (42.4%). Lastly, tuberculosis bacterium culture ascertained 75 cases (81.5%). Conclusion Bronchoscopy of local lesion with brush, lavage and biopsy is the most sensitive and specific diagnostic method to diagnose tracheobronchial tuberculosis. It has great clinical value in preventing tracheobronchial tuberculosis misdiagnosis. Related Articles | Metrics

Select

Correlation of RBP4 and omentin-1with ischemic stroke and carotid artery stenosis LIU Yuan, HAN Yanqing, LI Qian, ZHAO Yu, WANG Yiyi, LU Liandi 2015, 43 (12):  1424-1427.  doi: 10.11958/j.issn.0253-9896.2015.12.021 Abstract ( 636 )   PDF (316KB) ( 4098 )   Objective To investigate the correlation of serum levels of retinol binding protein 4 (RBP4) and andomen⁃ tin-1 with ischemic stroke and carotid artery stenosis. Methods Healthy adult (n=50) were selected as the control group while ischemic stroke patients with different degrees of carotid artery stenosis (n=143) were enrolled as the disease group. According to the ultrasonic Doppler performance, these ischemic stroke patients were divided into three groups: mild steno⁃ sis group (carotid artery stenosis was normal or less than 50%, n=67), moderate stenosis group (carotid artery stenosis was 50% to 70%, n=45), severe stenosis group (carotid artery stenosis was 70% to total occlusion, n=31). BMI index, hyperten⁃ sion, total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and fasting blood glucose (FPG) were detected by Automatic biochemical analyzer and serum levels of RBP4 and omentin-1 were measured by ELISA. Results Hypertensive population in carotid artery stenosis group was larger than that in control group (P＜0.05). Serum TC, TG, LDL, FPG and RBP4 levels were significantly higher while omentin-1 level decreased remarkably in disease group than those in the control group (P＜0.05). Logistic regression analysis showed that TC and RBP4 were the risk factors of ischemic stroke with carotid artery stenosis, while omentin-1 was a protective factor. Conclusion RBP4 is a risk factor for ischemic stroke with carotid artery stenosis and omentin-1 is a protective factor. Related Articles | Metrics

Select

Effect of PCI after thrombolysis and primary PCI on the treatment of acute ST elevation myocardial infarction CHEN Wanwan, YAO Zhuhua, MA Mei, CHENG Lisong, ZHOU Xin, DU Jianping, PANG Zhihua 2015, 43 (12):  1428-1432.  doi: 10.11958/j.issn.0253-9896.2015.12.022 Abstract ( 1200 )   PDF (369KB) ( 3854 )   Objective To investigate the differences in clinical efficacy and safety between thrombolysis followed PCI (percutaneous coronary intervention) and primary PCI in patients with acute STEMI（ST elevation myocardial infarction）. Methods A total of 215 STEMI patients who visit our clinic within 12 h since onset of their symptoms from May 2013 to January 2015 were enrolled. All eligible patients were divided into Early PCI group(n=68) and pPCI group (n=147) based on whether or not they received injection of recombinant human prourokinase thrombolytic therapy before their visit. Immediate TIMI (Thrombolysis In Myocardial Infarction) flow grade of infarct-related artery (IRA) before and after PCI treatment, post⁃ operative CTFC (Corrected TIMI Frame Count) and TMPG (TIMI myocardial perfusion grade) were compared between these two groups. The incidence of bleeding during hospital stay , left ventricular function at 6 month after intervention and major adverse cardiac events (MACE) were all observed. Rusults There is no obvious difference between the baseline of two groups. Before PCI, the proportion of TIMI grade 2-3 was higher in Early PCI group (77.9% vs 20.4%,P < 0.05）than that in pPCI group; but there was no significant difference in the proportion of TIMI grade 2-3 between these two groups after PCI (P > 0.05). CTFC and peak value of serum CK-MB were lower [(27.7±5.0) vs (32.6±7.1), P < 0.05; (225.8±108.3) U/L vs (283.4±110.6) U/L, P < 0.05] and rate of TMPG 3 is higher (82.4% vs 68.7%, P < 0.05）in Early PCI group than those in pPCI group. No significant difference was found in the incidence of bleeding and MACE during hospital stay and Left ventric⁃ ular function at 6 months after operation between these two groups. By contrast, LVEFs were higher while LVEDds (LVED diameter) were lower after 3 and 6 months of the intervention compared to those before intervention in both groups (P < 0.05). Conclusion It is a safe and effective reperfusion strategy for STEMI patients to receive rhPro-UK thrombolytic thera⁃ py followed early PCI as an alternative way to those who failed to receive pPCI on time. It didn′t increase the occurrence of bleeding complications and MACE, and at the same time it presented the same benefit in improving recent cardiac function as pPCI did. Related Articles | Metrics

Select

A prospective study of the relationship between alcohol consumption and outcome of three-year pre-diabetes CHE Xiaoli，WAN Qin 2015, 43 (12):  1433-1436.  doi: 10.11958/j.issn.0253-9896.2015.12.023 Abstract ( 628 )   PDF (296KB) ( 3730 )   Objective To investigate the associations of alcohol consumption with the outcome of three-year pre-dia⁃ betes. Methods The objects of this study were selected from the epidemiological study of cancer risk of Chinese patients with type 2 diabetes mellitus (T2DM) in Luzhou area. A total of 1 125 cases with pre-diabetes were selected from the base⁃ line survey. Patients divided into four groups including non-alcohol drinking group, small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. Patients also were divided into two groups according to body mass index (BMI, more than 24 kg/m2). The patients were followed up for three years. The incidence rates of diabetes mellitus were compared between different drinking groups and different BMI groups. Results A total of 1 116 cases were completed the follow-up , 9 cases were lost to follow-up. The incidence rates of DM were 18.81%, 8.80%, 16.51% and 26.53% for non-al⁃ cohol drinking group, small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. Cox proportional hazard regression analysis showed that the relative risk ratios and 95%CI of the gender, age, BMI, calorie, base⁃ line blood glucose were 0.49 (0.31-0.79), 1.15 (0.69-1.92) and 1.95 (1.25-3.04) for small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. In the group of BMI < 24 kg/m2，the relative risks and 95%CI were 0.40 (0.20-0.80)，0.77 (0.30-1.97) and 1.10 (0.46-2.64) for small alcohol drinking group, moderate alcohol drinking group, and large alcohol drinking group. In the group of BMI≥24 kg/m2, the relative risks and 95%CI were 0.62 (0.33-1.66)，1.49 (0.80-2.80) and 2.64 (1.55-4.47). Conclusion Small amount of alcohol drinking can reduce the incidence of DM in predia⁃ betes people with BMI<24 kg/m2. Large amount of alcohol drinking is the risk factor of incidence of DM in people with BMI≥ 24 kg/m2. Related Articles | Metrics

Applied Essay

Select

Effect of CTP guided thrombolytic therapy in the treatment of acute cerebral infarction GAO Luyan, WANG Hongxin, LIANG Peifen, DONG Yinhua, ZHAO Lan, TANG Jiangwei, LI Qiang, FAN Hongguang, ZHOU Lina 2015, 43 (12):  1437-1439.  doi: 10.11958/j.issn.0253-9896.2015.12.024 Abstract ( 950 )   PDF (346KB) ( 3883 )   Objective To investigate the effect of CT perfusion (CTP) imaging guidance in the treatment of acute cere⁃ bral infarction. Methods Patients (n=200) with acute cerebral infarction who visited our clinic within 6 hours underwent CTP examination and were divided into two groups: penumbra group and non-penumbra group according to their CTP imag⁃ ing (presence of penumbra or not). Recombinant tissue plasminogen activator (rt - PA) was administrated for intravenous thrombolysis in both groups. NIHSS (The NIH Stroke Scale), BI (Barthel Index), mRS (modified Rankin Scores) and hemor⁃ rhagic transformation events of two groups were determined before and after thrombolysis to evaluate its effect and prognosis in these two group. Results Compared with non penumbra group, NIHSS was reduced in penumbra group from 7 days after rt-PA (6.67±3.46 vs 4.76±2.04), and this decrease became obvious at 4 weeks after rt-PA (6.67±3.46 vs 3.68±1.93). Effi⁃ ciency rate at 4 week (60.3%) and good prognosis rate at 3 months（71.7%）were both significantly improved in penumbra group than those in non penumbra group（34.7%，56.8%）. Conclusion rt-PA under CTP guidance is effective and safe in the treatment of acute cerebral infarction. The thrombolytic therapy window can be enlarged according to the presence of pen⁃ umbra or not and the bleeding conversion rate remains at low level. Related Articles | Metrics

Select

Clinical pedicle internal fixation combined with debridement and bone graft through intervertebral approach as treatment of segmental lumbosacral vertebral tuberculosis QIU Nanhai, WANG Li，MIAO Ruirui，XUE Haibin 2015, 43 (12):  1440-1443.  doi: 10.11958/j.issn.0253-9896.2015.12.025 Abstract ( 749 )   PDF (599KB) ( 3933 )   Objective To discuss and observe the clinical effect of intervertebral pedicle internal fixation and debride⁃ ment combined with bone graft through posterior approach/trans-intervertebral space approach on the treatment of uni/multisegmental lumbosacral vertebral tuberculosis (TB). Methods A cohort of 37 patients, with single or multiple segmental ver⁃ tebral destruction due to TB, were treated by trans-intervertebral debridement, posterior pedicle screw system internal fixa⁃ tion and intervertebral bone graft. All patients underwent X-ray，CT and MRI examination to observe the combination treat⁃ ment effect. Results Most patients (n=34) enjoyed primary healing, in which include only 4 cases that presented symptom of nerve root stretch injury during operation but all recovered after 3 months. Other 3 patients underwent secondary healing due to sinus but two were rectifying with anti-TB therapy and wound dressing. The other 1 case suffered from sinus tract was healed through second debridement and rectifying therapy. X-ray, CT and MR at 6 months after operation indicated that all patients present great graft osseous fusion, good recovering of height of vertebral body without kyphosis deformity nor internal fixation loosening nor screw breakage. Conclusion Intervertebral pedicle internal fixation and debridement combined with bone graft through posterior approach/trans-intervertebral space approach is with minimum invasion but good graft fusion ef⁃ fects,harderfixationandsatisfactoryclinicaleffectsinthetreatmentofuni/multi-segmentallumbosacralvertebraltuberculosis. Related Articles | Metrics

Select

The efficacy analysis of radiofrequency therapy combined with ozone injection in the treatment of lumbar disc protrusion LIU Tao, SHEN Cailiang, TANG Kaijun, LIN Yuchun, SUN Yong 2015, 43 (12):  1443-1446.  doi: 10.11958/j.issn.0253-9896.2015.12.026 Abstract ( 916 )   PDF (356KB) ( 3792 )   Abstract：Objective To assess the efficacy of radiofrequency therapy combined with ozone injection in the treatment of lumbar disc protrusion. Methods A total of 202 patients with lumbar disk protrusion were included and divided into three groups: radiofrequency (RF) group, ozone (Oz) group, and combination (Co) group. The efficacy was evaluated by visual analogue scale (VAS), Macnab curative effect evaluation, and oswestry disability index (ODI) at 1d, 1m and 6m after treat⁃ ment. Results There were no significant differences in gender, age, the course of disease, preoperative VAS scores and ODI scores (P > 0.05). All patients were operated successfully. There were no infection of the intervertebral disc, no compli⁃ cations of spinal cord, nerve and no blood vessel injury. There were no significant differences in preoperative VAS and ODI scores between three groups. The VAS and ODI scores were significantly lower at 6 month and 12 month after operation in Co group combined with those of RF group and Oz group. There were no significant differences in these two scores between RF group and Oz group (P > 0.05). There were no significant differences in effective rates at 1d, 6 month and 12 month after operation between three groups (P > 0.05). But the excellent and good effective rates were higher in Co group than those of RF and Oz groups. There were no significant differences in excellent and good effective rates between RF group and Oz group. Conclusion The therapy of radiofrequency combined with ozone injection is an efficient and mini-invasive tech⁃ nique for the treatment of lumbar disc protrusion and worth promoting. Related Articles | Metrics

Select

Effect of various anaesthesia depth on the sublingual microcirculation LIU Zhihui, WANG Guolin, LI Peibo, XIE Keliang, LIU Lingling, YU Yonghao 2015, 43 (12):  1447-1449.  doi: 10.11958/j.issn.0253-9896.2015.12.027 Abstract ( 761 )   PDF (291KB) ( 3950 )   Objective To investigate the effects of different depth of anesthesia on sublingual microcirculation. Meth⁃ ods ASA grade Ⅰ-Ⅱ patients (n=20) were scheduled for elective thyroid surgery and included in the prospective observa⁃ tional study. Midazolam 0.05 mg·kg, sufentanil 0.3 μg·kg-1 and rocuronium 0.6 mg·kg-1 were administrated intravenously to induce anesthesia which was then maintained by continuous intravenous infusion of propofol. Target medication concentra⁃ tion increased 0.5 mg·L-1, regulated based on BIS. The patients underwent endotracheal intubation and mechanical ventila⁃ tion. Sublingual microcirculations were evaluated by sidestream dark field (SDF) imaging at T1 (BIS baseline ), T2 (50 < BIS ≤ 60), T3 (40 0.05). Conclusion When BIS value sit between 40 and 50, it can best inhibit stress response and attenuate the agitation of microcirculation. Related Articles | Metrics

Drug Clinical Evaluations

Select

Comparison of the effects of intravenous pre-treatment of Butorphanol and Dezocine on prevention of Etomidate-induced myoclonus ZHANG Jing, LIU Ling，LYU Guoyi 2015, 43 (12):  1450-1453.  doi: 10.11958/j.issn.0253-9896.2015.12.028 Abstract ( 668 )   PDF (359KB) ( 3879 )   Objective To compare the inhibitory effects of Butorphanol and Dezocine on Etomidate-induced myoclo⁃ nus. Methods A total of 150 patients with ASA physical statusⅠorⅡ, aged 40-65 yr, with body mass index (BMI) of 20- 25 kg/m², scheduled for elective operations under general anesthesia, were included in this study. Patients were randomly al⁃ located into three groups (A, B and C) with 50 patients in each group. Group A was given intravenous Butorphanol 15 μg/kg for 30 s, group B was given Dezocine 0.1 mg/kg and group C was given equal volume of saline. After 2 min, etomidate 0.3 mg/kg was administrated to three groups. The occurrence and severity of myoclonus were recorded for 2 min after administration of Etomidate. The mean arterial pressure (MAP), heart rate (HR), pulse oxygen saturation (SpO2) and Bispectral index (BIS) were recorded at the time points before induction (T0), 2 min after the experimental drug treatment (T1), and 2 min after Etomi⁃ date treatment (T2). At the same time, the concentration of serum potassium was determined at T0 and 5 min after endotrache⁃ al intubation (T3) respectively. Results The positive incidences of myoclonus were 12% in group A, 22% in group B and 74% in group C, respectively. Compared with group C, the positive incidence rates of myoclonus and myoclonus scales were significantly lower in group A and group B (P < 0.05), but no significant difference between group A and group B (P > 0.05). Compared with T0, there was no significant difference in the potassium concentration between patients without myoclonus (grade 0) and patients with myoclonus (grade 1 and grade 2) at T3 (P > 0.05). There was a significant increase in potassium concentration in patients with grade 3 (P < 0.05). There were no significant differences in MAP, HR, SpO2 and BIS values at T0, T1 and T2 between three groups of patients (P＞0.05). Conclusion Pre-treatment of Butorphanol (15 μg/kg) or Dezocine (0.1 mg/kg) can reduce the Etomidate-induced myoclonus. At the same time, both therapies show no different effects on cir⁃ culation and respiration system. Related Articles | Metrics

Select

The effects of losartan potassium and simvastatin combination therapy on oxidative stress indicators in diabetic patients on peritoneal dialysis LIU Hua, LIU Cuilan, CHENG Jinxiu, LU Jifang, LIU Shengjun 2015, 43 (12):  1453-1455.  doi: 10.11958/j.issn.0253-9896.2015.12.029 Abstract ( 796 )   PDF (290KB) ( 3736 )   Objective To explore the effects of losartan potassium and simvastatin combination therapy on oxidative stress indicators in diabetic patients on peritoneal dialysis through 12 weeks observation. Methods Diabetic patients with end-stage nephropathy (n=80) who were treated with continuous ambulatory peritoneal dialysis were randomly divided into two groups: control group who received routine treatment (n= 40), treatment group who were given losartan potassium 50 mg, once per day and simvastatin 20 mg, once every night (n=40). HbA1C, Insulin dosage, Oxidative stress indicators（SOD, GSH-PX, MDA and Hcy）were compared between two groups before and after peritoneal dialysis. Results There was no significant difference of HbA1C between the 2 groups before and after treatment（P＞0.05）. The insulin doses increased be⁃ fore dialysis in both groups after CAPD treatment. It is lower in the treatment group than that in the control group (P＜0.05). The expression levels of GSH-PX and SOD in treatment group were higher while the expressions of Hcy and MDA were lower after treatment. The expressions of GSH-PX and SOD were higher while the expressions of Hcy and MDA were lower in treat⁃ ment group than those in control group when comparing the same time point（P＜0.05）. GSH-PX expression level was lower while the expressions of MDA and Hcy were higher after dialysis than those before dialysis in control group (P＜0.05). Con⁃ clusion Losartan potassium combined with simvastatin treatment can improve curative effect and oxidative stress indicators in diabetic patients on peritoneal dialysis. Related Articles | Metrics

Review

Select

Current research of L-amino acid transporter 1 in tumor diagnosis and treatment DONG Yan,WU Weidang,CI Xiaoyan,GAO Jing,ZENG Yong,YI Xiulin 2015, 43 (12):  1456-1459.  doi: 10.11958/j.issn.0253-9896.2015.12.030 Abstract ( 1163 )   PDF (362KB) ( 4482 )   L-amino acid transporter 1 ( LAT1) is a member of L-amino transporter family and an important heterodi⁃ meric amino acid transporter that belongs to subfamily of SLC7. LAT1 mainly mediates trans-membrane transportation of those neutral amino acids that had cyclobenzene or long side chains with high molecular mass such as L-Leu, L-Met and LPhe as well as some amino acid analogues such as melphalan, L-DOPA and thyroxine in a Na+ and ATP-independent diffu⁃ sion. As LAT1 was abnormally overexpressed in various transformed cell lines, it might be related with tumor stage and prog⁃ nosis. It is not only a biomarker that specifically expressed in some tumor cells but also plays an important role in tumor diag⁃ nosis and therapy. Here we demonstrate the structure of LAT1 and its mechanism in transport peculiarity. We also reviewed the development of LAT1 in tumor diagnosis and treatment. Related Articles | Metrics

Select

Current research in roles of CD38,CD138 and IgG4 in the pathogenesis of biliary atresia GAO Ting, ZHAN Jianghua 2015, 43 (12):  1460-1463.  doi: 10.11958/j.issn.0253-9896.2015.12.031 Abstract ( 879 )   PDF (317KB) ( 4281 )   Biliary atresia (BA) is one of the most serious pediatric surgical digestive system diseases with progressive liv⁃ er bile duct inflammation and fibrous obstruction. Currently, the etiology of BA is not clear. It may be associated with genetic predisposition, viral infections and immune injury. Now many scholars believe that it was resulted from multiple factors. Among them, the theory of immune-inflammatory is supported by most scholars. Now the mechanisms of CD38, CD138 and IgG4 in autoimmune liver disease were reported in literature. BA and other autoimmune liver diseases are similar in terms that both inflammatory and immune responses plays irreplaceable role during disease development. Therefore, this article briefly review the role of CD38, CD138 and IgG4 in the inflammation-immunity of BA. Related Articles | Metrics

Select

The relationship between Prdx1 and carcinoma GAO Jun, ZHU Hong, GAO Guolan 2015, 43 (12):  1464-1466.  doi: 10.11958/j.issn.0253-9896.2015.12.032 Abstract ( 1045 )   PDF (284KB) ( 4055 )   Peroxiredoxin 1（Prdx1）belongs to peroxide oxidoreductase protein（peroxiredoxin, Prdx）family，which is over-expressed in multiple cancers and it play an important role in antagonizing reactive oxygen species (ROS) therefore as works as anti-oxidations. Its expression is closely related with tumor proliferation, differentiation and metastasis as well as with sensibility to radiotherapy and chemotherapy. Its structure and biological function in tumors and regulation mechanism were reviewed in this paper. It can provide evidence to screen new therapeutic targets in the treatment of tumors. Related Articles | Metrics

Select

Current research of the role of Sox2 in Glioblastoma Multiforme GUO Jinman，TAN Chao，HU Huojun，TAN Yuan 2015, 43 (12):  1467-1469.  doi: 10.11958/j.issn.0253-9896.2015.12.033 Abstract ( 653 )   PDF (279KB) ( 3926 )   Glioblastoma (GBM) is one of the WHO grade Ⅳmalignancies, which is an acentral nervous system cancer with poor prognosis unless the lesion is in the brain stem. The incidence of GBM accounts for 80% of human primary malignant tumors in brain. Only 5% GBM can survive up to 5-years. Many researches showed that Sox2 is a pluripotent regulator, and muta⁃ tion or abnormal function of Sox2 are closely related to the development of GBM. There are studies demonstrated the possibil⁃ ity of using Sox2 gene as apotential target for GBM therapy. This paper reviewed recent progress in GBM. References | Related Articles | Metrics

Select

Autophagy: an active participant in the development of diabetes ZHANG Qiong, HUANG Xiaofei, ZHAI Wenhai, YANG Deyuan 2015, 43 (12):  1470-1472.  doi: 10.11958/j.issn.0253-9896.2015.12.034 Abstract ( 808 )   PDF (275KB) ( 4548 )   The pathogenesis of diabetes is complicated by several factors including autoimmunity, environment, heredi⁃ ty, and etc. Autophagy is a kind of intracellular biodegradation processes, which plays an important role in intracellular ho⁃ meostasis of islet cells. In diabetes, autophagy is involved in the endoplasmic reticulum stress, mitochondrial dysfunction and inflammatory, and it affects the development of the disease. In this paper, we reviewed the interactions of autophagy with endoplasmic reticulum stress and mitochondrial dysfunction with inflammation in diabetes in order to investigate the patho⁃ genesis of diabetes, to find new strategies for prevention or treatment of diabetes. Related Articles | Metrics