Abstract: Abstract:Objective To investigate the effects and mechanisms of bone marrow mesenchymal stem cells (BMSCs) on pancreatic fibrosis in rats of chronic pancreatitis. Methods Thirty healthy male SD rats were randomly divided into three groups: control group, model group and transplanted group (n=10 for each group). Chronic pancratitis rat model was induced by retrograde injection of oleic acid into the biliopancreatic duct. The sham operation group was treated only with solvent. Transplanted group was given BMSCs through caudal vein injection at 1 week and 5 weeks after the model induction. All rats were weighed at 1 week, 4 weeks and 8 weeks in three groups. After 8-week feeding， pancreatic tissues were harvested for HE and picric-sirius staining. The contents of transforming growth factor β1 (TGF-β1), type Ⅰ collagen and type Ⅲ collagen were detcted by using ELISA. Results Compared with the control group, the weights of rats were decreased at 4 weeks and 8 weeks in model group and transplantated group (P＜0.05). There were no significant differences in body weights between model group and transplantated group (P＞0.05). The pancreatic fibrosis score and pathological injury were ameliorated signicantly in transplanted group. The contents of TGF-β1, type Ⅰ collagen and type Ⅲ collagen in pancreas were increased in model group than those of control group and transplanted group (P＜0.05). Conclusion BMSCs transplantation can reduce the collagen secretion and reduce the degree of pancreatic fibrosis in rats with chronic pancreatitis, which may be related to the inhibition of the release of TGF-β1

Key words: 胰腺炎, 慢性, 纤维化, 转化生长因子β1, 胶原Ⅰ型, 胶原Ⅲ型, 骨髓间充质干细胞