Abstract: Abstract Objective： This study was aim to investigate the effects of iridoid on the spermatogenesis functions of type-2 diabetes induced by nicotinamide and streptozotocin. Methods：56 male C57BL mice were divided into seven groups (n=8) randomly: control; diabetic mice; diabetic mice administered glibenclamide (0.25 mg/kg); diabetic mice who received the iridoid(20 and 40mg/kg groups); and normal mice received iridoid(20 and 40mg/kg)groups. Diabetic model was induced by intraperitoneal?injection of streptozotocin (65 mg/kg) and nicotinamide (120 mg/kg). Glibenclamide and iridoid were orally feed for the next 28 days. 24 hours after the last treatment, blood samples, testis and the epididymis were obtained for testis, sperm analysis and hormonal tests. Results: Body and testicular weight, sperm count and viability, and LH, FSH, T levels were significantly decreased in diabetic group(p<0.05). The diabetic mice treated with 40 mg/kg of iridoid recovered from these reductions(p<0.05). Glibenclamide relieved hormonal and sperm reduction in diabetic mice group(p<0.05). Conclusions：These results suggests that the iridoid has anti-infertility effects in diabetic males mice.

Key words: diabetes mellitus, type 2, iridoides； luteinizing hormone, follicle stimulating hormone, testosterone, spermatozoa, mice, inbred C57BL, antioxidant