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15 March 2021, Volume 49 Issue 3 Previous Issue    Next Issue

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Effects of miR-124 targeting chemokine-like factor superfamily member 6 on glioma cells LIANG Bo, WANG Xin-jun△, WANG Jian-ye, ZHOU Shao-long, YANG Zhuo 2021, 49 (3):  225-230.  doi: 10.11958/20202275 Abstract ( 1026 )   PDF (831KB) ( 5009 )   Objective　To investigate the inhibitory effect of microRNA (miR)-124 targetingly regulating chemokine-like factor super family 6 (CMTM6) on the invasion and migration of glioma cells. Methods　The levels of miR-124 and CMTM6 mRNA in OLN93, SHG-44, U87, U251 and A172 cells were detected by real-time fluorescence quantitative PCR (qPCR). The levels of CMTM6 protein in OLN93, SHG-44, U87, U251 and A172 cells were detected by Western blot assay. The miRTarBase was used to predict the binding sites of miR-124 and CMTM6. There were four experimental groups in this study: mimic NC group, miR-124 mimic group, miR-124 mimic+pcDNA group and miR-124 mimic+CMTM6 group. The mimic NC, mimic miR-124, pcDNA and pcDNA+CMTM6 were transfected into each cell group. The levels of miR-124 and CMTM6 mRNA were detected by qPCR. CCK-8 was used to detect the cell proliferation. Transwell test was used to detect cell invasion and migration. The cell migration was detected by scratch test. The protein levels of CMTM6 and cell surface programmed death protein ligand 1 (PD-L1) were detected by Western blot assay. Results　Compared with OLN93 group, the levels of miR-124 were lower in SHG-44, U87, U251 and A172 groups (P＜0.05). The levels of CMTM6 mRNA and protein were higher (P＜0.05). U251 cells were selected for subsequent experiments. miRTarBase predicted that there was a binding site between miR-124 and CMTM6, which was verified by double luciferase reporter gene experiment. Compared with that in mimic NC group and miR-124 mimic+CMTM6 group, the levels of miR-124 in miR-124 mimic group and miR-124 mimic+pcDNA group were higher (P＜0.05), and the level of CMTM6 mRNA was lower. After cells were cultured for 24, 48, 72, 96 h, the cell OD450 decreased, the numbers of invasion and migration cells decreased, the protein levels of CMTM6 and PD-L1 were lower (P＜0.05). Conclusion　Increasing the expression of miR-124 can down-regulate the expression of CMTM6, thereby inhibiting the invasion and migration of glioma cells. Related Articles | Metrics

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The mechanism of PD-L1 overexpression in DENV-2 induced vascular endothelial cell autophagy and apoptosis YAOFeng, ZHULei, CHENGBo, YANGMing, LIUMin△ 2021, 49 (3):  231-236.  doi: 10.11958/20201709 Abstract ( 707 )   PDF (748KB) ( 5288 )   Objective　To investigate the role and mechanism of PD-L1 overexpression in the effect of dengue virus type Ⅱ (DENV-2) on vascular endothelial cell autophagy and apoptosis. Methods　The vascular endothelial cell EAhy926 was used as the research object. The cells were treated with serum-free medium containing DENV-2 (1×108-2×109 pfu/L) for 24 h and 48 h. At the same time, cells were treated with serum-free medium as control group. Add serum-free medium to blank holes as zero adjustment holes. The cell proliferation activity was detected by MTT method. The cells were treated with PD-L1 overexpression lentiviral solution and no-load lentiviral solution, and cells were set as no-load control group and transfection group respectively. Cells treated with DENV-2 (1.2×109 pfu/L) for 12 h, 24 h and 48 h were set as DENV-2 12-h group, DENV-2 24-h group and DENV-2 48-h group, respectively. At the same time, the control group was set (cells treated with serum-free medium). In addition, the no-load control group and the transfection group were treated with DENV-2 for 48 hours, and the protein expression changes of LC3B, Beclin-1 and Bcl-2 were detected by Western blot assay. The cell apoptosis was detected by flow cytometry. Results　1×108-2×109 pfu/L DENV-2 can inhibit the proliferation of EAhy926 cells in a dose-dependent manner, with IC50 of 1.8×109 pfu/L and 1.2×109 pfu/L, respectively. Compared with the control group, the expression leves of LC3Ⅱ/LC3Ⅰ and Beclin-1 protein were up-regulated in the DENV-2 12-h group DENV-2 24-h group and DENV-2 48 h group, the expression of Bcl-2 protein was down-regulated, and the total cell apoptosis rate was increased (P＜0.05). Compared with the empty control group, the expression of PD-L1 protein was up-regulated in the transfection group (P＜0.01). After DENV-2 treatment for 48 hours, compared with the no-load control group, the expressions of LC3Ⅱ/LC3Ⅰ and Beclin-1 protein were down-regulated in the transfection group, the expression of Bcl-2 protein was up-regulated, and the total cell apoptosis rate was reduced (P＜0.01). Conclusion　DENV-2 induces autophagy and apoptosis in EAhy926 cells by up-regulating LC3Ⅱ/LC3Ⅰ, Beclin-1 protein expression and down-regulating Bcl-2 protein expression, while the overexpression of PD-L1 can resist the inducing effect of DENV-2. Related Articles | Metrics

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Effects and mechanism of luteolin on the proliferation and apoptosis of K562 cells PENGYan-hui, DUANZhi, LITao△ 2021, 49 (3):  236-241.  doi: 10.11958/20202294 Abstract ( 838 )   PDF (589KB) ( 5174 )   Objective　To investigate the effects of luteolin on proliferation and apoptosis in K562 cells and the underlying molecular mechanism. Methods　The K562 cells in logarithmic growth phase were divided into control group, 10 μmol/L luteolin group, 25 μmol/L luteolin group, 50 μmol/L luteolin group and 100 μmol/L luteolin group, respectively. The effect of luteolin on the proliferation of K562 cells was detected by CCK8 assay, and the effect of luteolin on the apoptosis of K562 cells was detected by flow cytometry. The expression levels of Bax, Bcl-2, PARP, Cleaved-PARP, Caspase3, Cleaved-Caspase3, AKT, p-AKT and BCR-ABL were detected by Western blot assay. Results　CCK-8 assay showed that the inhibition ratio of K562 cells gradually increased with the increased luteolin concentrations and intervention time (P＜0.05). Western blot assay showed that the proliferation inhibition rate of K562 cells increased with the increased luteolin concentration and time (P＜0.05). The flow cytometry results showed that the apoptosis rates of K562 cells were increased in turn after treatment with luteolin 0, 25 and 50 μmol/L (8.21%±0.55%, 23.43%±1.50%, and 40.47%±2.97%, respectively). Western blot results showed that the expression levels of Bax, Cleaved-Caspase3 and Cleaved-PARP increased with the increasing of luteolin concentration (P＜0.05). The expression levels of PARP were increased in turn in the luteolin 0 μmol/L, 10 μmol/L and 50 μmol/L groups (P＜0.05). There were no significant difference between the 50 μmol/L group and 100 μmol/L luteolin group. The protein expression levels of Caspase3 and Bcl-2 were significantly lower in 100 μmol/L group than those in other groups (P＜0.05). The expression levels of p-AKT were lower in 50 μmol/L and 100 μmol/L groups than those in 0 μmol/L and 10 μmol/L groups. And the expression level of p-AKT was lower in 100 μmol/L group than that in 50 μmol/L group. The expression level of BCR-ABL was significantly higher in 50 μmol/L group than that in 0 μmol/L group. The expression level of BCR-ABL was significantly lower in 100 μmol/L group than that in 0 μmol/L and 50 μmol/L groups (P＜0.05). Conclusion　Luteolin can inhibit the proliferation of K562 cells and induce the apoptosis of K562 cells, which may be related to the regulation of BCR-ABL protein expression and PI3K/AKT signaling pathway. Related Articles | Metrics

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Study on the function and mechanism of miR-208a in promoting the proliferation, invasion and migration of non-small cell lung cancer A549 cells LIXin-ling, WUZhen-guo, ZHANGLi-hai, ZHUQi-feng△ 2021, 49 (3):  242-247.  doi: 10.11958/20202500 Abstract ( 860 )   PDF (911KB) ( 5377 )   Objective　To investigate the functional role and mechanism of miR-208a on proliferation, invasion and migration of non-small cell lung cancer (NSCLC) A549 cells. Methods　(1) A549 cells were transfected with miR-208a mimic, NC mimic, miR-208a inhibitor and NC inhibitor, respectively, and the overexpression and interference efficiency of miR-208a were detected by quantitative polymerase chain reaction (qPCR). CCK-8, Transwell and wound healing assays were used to evaluate the effects of miR-208a on proliferation, invasion and migration of A549 cells. Bioinformatics websites such as miRDB, PicTar, Targetscan and miRanda were used to predict the downstream target genes of miR-208a. PTPRG 3'UTR wild-type and mutant double luciferase reporter gene vectors were constructed and co-transfected with miR-208a into HEK-293T cells to observe the changes of luciferase activity. (2) A549 cells in logarithmic phase were taken, and negative control group (si-NC group), PTPRG knockdown group (si-PTPRG group), PTPRG knockdown group + miR-208a interference group (si-PTPRG + miR-208a inhibitor group) were set up. CCK-8, Transwell test and scratch test were used to detect the effects of PTPRG on proliferation, invasion and migration of A549 cells. qPCR and Western blot assays were performed to detect the expression levels of PTPRG mRNA and protein. Results　There were increased levels of miR-208a mRNA expression, cell proliferation, migration and invasion in miR-208a mimic group compared with those of NC mimic group (P＜0.05). By contrast, miR-208a inhibition exerted the opposite effects. Bioinformatics analysis showed that PTPRG was the target gene of miR-208a, and the overexpression of miR-208a could reduce the luciferase activity of PTPRG 3'UTR wild-type, and miR-208a inhibitor could increase the luciferase activity of PTPRG 3'UTR wild-type, indicating that miR-208a could bind to the 3'UTR sequence of PTPRG. Further studies showed that compared with si-NC group, the proliferation, invasion and migration of cells were significantly increased in si-PTPRG group, and the expression levels of PTPRG gene and protein were decreased (P＜0.05). Compared with si-PTPRG group, the proliferation, invasion and migration ability were decreased in si-PTPRG + miR-208a inhibitor group, and the expression levels of PTPRG gene and protein were increased. Conclusion　miR-208a promotes the proliferation, invasion and migration of A549 cells by targeting PTPRG. Related Articles | Metrics

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Study on the mechanism of moxibustion in attenuating hypoxia-ischemic brain injury in neonatal mice OUYANGXin, XUYou-hui, CHENMing-ren, XIAOAi-jiao△ 2021, 49 (3):  248-252.  doi: 10.11958/20202682 Abstract ( 772 )   PDF (682KB) ( 4922 )   Objective　To investigate the mechanism of moxibustion on hypoxia-ischemic encephalopathy (HIE) in neonatal mice. Methods　A total of 55 neonatal ICR mice (aged 7 days) were randomly divided into sham-operated group (Sham group), HI group and moxibustion-treated (MOX) group according to the method of random digital table. Mice in both HI group and MOX group were induced HIE model by ligation of the right common carotid artery combined with hypoxia in a closed transparent chamber. After the model was established, mice in MOX group were given moxibustion treatment by heating Dazhui points 35 min/d, once a day for 3 days. The morphological structure of brain tissue was examined by DAPI staining. The apoptotic cells of brain tissue were detected using TUNEL assay. The expression of caspase-9 was detected by immunofluorescent staining, and the content of caspase-3 was determined by radioimmunoassay. Results　Compared with the Sham group, mice in the HI group showed that the arrangement of brain tissues was loose, and the number of apoptotic cells, the expression of caspase-9 positive cells in brain tissues increased (P＜0.05). Compared with the HI group, mice in the MOX group exhibited that the arrangement of brain tissues was relatively compact, and the number of apoptotic cells, the expression of caspase-9 and the content of caspase-3 in brain tissues decreased significantly (P＜0.05). Conclusion　Moxibustion may decrease caspase-9 expression and caspase-3 content of brain tissues and then reduce apoptosis, thus reducing hypoxic-ischemic brain injury in neonatal mice. Related Articles | Metrics

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The protective effect of paeonol on IL-1β induced inflammatory response of human fibroblast-like synoviocytes and rheumatoid arthritis LIMei, OUDa-ming, HUANGLi-fang, XIELi-hu, ZHANGJi 2021, 49 (3):  252-257.  doi: 10.11958/20201131 Abstract ( 687 )   PDF (1011KB) ( 4679 )   Objective　To explore the protective effect of paeonol on IL-1β induced inflammatory response of human fibroblast-like synoviocytes and rheumatoid arthritis (HFLS-RA) and its possible mechanism. Methods　The HFLS-RA cells were divided into control group, 10 μmol/L paeonol group and 0.1, 1, 10 and 100 μmol/L HFLS-RA groups. The effect of paeonol on the proliferation of HFLS-RA was detected by MTT. The levels of TNF-α and IL-6 in HFLS-RA were detected by enzyme linked immunosorbent assay (ELISA). The nuclear translocation of NF-κB p65 was observed by immuno-fluorescence in blank control group, 10 μg/L IL-1β group and 10 μmol/L paeonol group. The expressions of MMP-1, MMP-3 and TLR4 groups in blank control group, 10 μg/L IL-1β group, 0.1, 1, 10 μmol/L paeonol and negative control group (only treated with 10 μmol/L paeonol) were detected by Western blot assay. Twenty-four male DBA/1 mice were randomly divided into control group, CIA group and treatment group. The limb cumulative score was evaluated. The pathological changes of joint tissues were observed by HE staining. The contents of TNF -α and IL-1β in synovial tissue were detected by ELISA. Results　Paeonol showed no inhibitory effect on IL-1β induced HFLS-RA proliferation. Compared with the blank control group, the expression levels of TNF-α, IL-6, NF-κB p65 nuclear translocation, MMP-1, MMP-3 and TLR4 protein were increased in 10 μg/L IL-1β group, and these changes could be reversed by paeonol intervention (P＜0.05). Compared with the CIA group, the limb cumulative score decreased significantly in the treatment group (P＜0.05). HE observation showed that the pathological changes of ankle joint were improved after 48 days of paeonol treatment, in which the inflammatory cells decreased significantly and the cartilage damage was alleviated. The levels of TNF-α and IL-1β in synovial tissue were lower in treatment group than those of CIA group. Conclusion　Paeonol can reduce the IL-1β induced expression levels of inflammatory factors in HFLS-RA and relieve arthritis symptoms in CIA mice, which may be related to the down regulation of TLR4-NF-κB p65 pathway. Related Articles | Metrics

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Effects of fecal microbiota transplantation on Keap1-Nrf2/ARE signaling pathway in rats with acute lung injury induced by endotoxin TANYi-min, WANGYu-lei, LIBo, YINGuo-fang, FANXian-ming△ 2021, 49 (3):  258-263.  doi: 10.11958/20202422 Abstract ( 651 )   PDF (918KB) ( 4831 )   Objective　To explore the role of Keap1-Nrf2/ARE signaling pathway in fecal microbiota transplantation (FMT) intervention in endotoxin-induced acute lung injury (ALI) in rats. Methods　Fifteen adult healthy male SD rats were randomly divided into control group (NS) , model group (LPS) and FMT group with 5 in each group. LPS group and FMT group were intraperitoneally injected with lipopolysaccharide (LPS) to build the model of ALI, and NS group was injected with the same volume of normal saline. After 24 h of modeling, self-made fecal fluid (10 mL/kg) was given to the FMT group (twice a day for 2 days), while the NS group and the LPS group were given the same volume of normal saline. After 24 h of the last FMT intervention, samples of celiac arterial blood, lung tissue and fresh fecal specimens were collected from each group. The lung wet/dry weight ratio (W/D), the arterial blood oxygen partial pressure［p(O)2］in celiac arterial blood, and the content of superoxide dismutase (SOD), malondialdehyde (MDA), protein carbonyl (PCO) in serum were measured by enzyme-linked immunosorbent (ELISA) method. Fresh fecal specimens were used for high-throughput sequencing of intestinal flora. HE staining was used to observe the changes of lung tissue and pathological score. Immunohistochemical detection was used for the nuclear factor-erythroid 2 related factor 2 (Nrf2) expression in lung tissue. Western blot assay was used to detect the expression of Keap1-Nrf2/ARE pathway related proteins. Results　Compared with NS group, the lung W/D and lung histopathological score were increased in LPS group, and p(O) 2 was decreased. At the same time, the serum content of SOD were lower in LPS group than that of NS group, while the serum levels of MDA and PCO and the expression levels of Nrf2, heme oxygenase-1 (HO-1) protein in lung tissue were increased in LPS group than those of NS group (all P＜0.05). Following treatment with FMT, the lung W/D and lung histopathological score decreased and p(O)2 increased in LPS group than those of FMT group, which accompanied by the increased content of SOD and the decreased levels of MDA and PCO. Notably, the expression levels of Nrf2 and HO-1 in lung tissue were significantly increased following the treatment with FMT (all P＜0.05). Compared with NS group and FMT group, the Beta diversity of the intestinal flora was different in LPS group, in which the Lactobacillaceae and Peptostreptococcaceae decreased and the Porphyromonadaceae increased significantly. Alpha diversity and Beta diversity of intestinal flora were similar in NS group and LPS group. Conclusion　FMT might attenuate LPS-induced lung injury in rats by regulating the intestinal flora acting on Keap1-Nrf2/ARE signal pathway, enhancing anti-oxidative stress response and reducing oxidative damage-related products. Related Articles | Metrics

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The potential effects of astragaloside on hypoxic pulmonary hypertension LIXu-dong, MAYong, DENGRun-peng, CANGXue-yu, WUFei-yan, LIYong-tao, JINHai-feng△ 2021, 49 (3):  264-268.  doi: 10.11958/20201704 Abstract ( 914 )   PDF (716KB) ( 5080 )   Objective　To explore the inhibitory effect of astragaloside (AST) on the pathological development of hypoxic pulmonary hypertension (HPH) and its relevant mechanism. Methods　Twenty-eight SPF male Sprague-Dawley rats were randomly divided into 4 groups (7 rats per group): control group, HPH group, HPH+AST15 [15 mg/(kg·d)] group, and HPH+AST45 [45 mg/(kg·d)] group. The rat model of HPH was established by intermittent normal pressure hypoxia. After 28-day hypoxia exposure, the right ventricle systolic pressure (RVSP) and the weight ratio of RV/(LV+S) were recorded. After HE and immunofluorescence staining, the percent medial wall thickness (WT), percent medial wall area (WA), and integrated optical density (OD) value of α-smooth muscle actin (α-SMA) in pulmonary arteries were determined to investigate pulmonary artery structural remodeling. The content of SOD, CAT and MDA both in serum and in lung tissues were measured by spectrophotometry. The NADPH (reduced nicotinamide adenine dinucleotide phosphate) oxidase Nox2 and Nox4 protein expressions in lung tissues were detected by Western blot analysis. Results　 Compared with those in control group, the RVSP, RV/(LV+S) ratio, WT, WA, and α-SMA value were increased significantly in HPH group (P＜0.05), SOD and CAT levels both in serum and in lung tissues were decreased significantly in association with the elevated MDA content (P＜0.05), and the Nox2 and Nox4 protein levels in lung tissues were increased significantly (P＜0.05). However, compared with HPH group, the RVSP, RV/(LV+S) ratio, WT, WA, and α-SMA value were decreased significantly (P＜0.05), SOD and CAT levels both in serum and in lung tissues were increased significantly in association with the decreased MDA content (P＜0.05), and the Nox2 and Nox4 protein levels in lung tissues were significantly decreased (P＜0.05) in HPH+AST15 group and HPH+AST45 group. In addition, the WT, WA, α-SMA value, MDA level in lung tissues, and Nox2 and Nox4 protein levels in lung tissues were significantly decreased in HPH+AST45 group than those of the HPH+AST15 group (P＜0.05). Conclusion　AST can attenuate the pathological development of HPH in rats, which may be associated with the elevating levels of SOD and CAT and inhibiting the expressions of Nox2 and Nox4 in rats. Related Articles | Metrics

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The protective effects of thymoquinone on the mouse hematopoietic system exposed to irradiation LIULin-lin 2021, 49 (3):  268-270.  doi: 10.11958/20201923 Abstract ( 654 )   PDF (333KB) ( 4387 )   Objective　To observe the effects of thymoquinone on the hematopoietic radiation injury in mice. Methods　Fifteen ICR mice were randomly divided into control group, irradiated group, and thymoquinone group according to body weight. The control group received sham irradiation, and the rest received 2.0 Gy total body irradiation. Thymoquinone was intragastrically administrated with 10 mg/kg 1 d before irradiation and continued for 3 days after irradiation, the other two groups were intragastrically administered with normal saline. After 2.0 Gy irradiation for 7 day, the peripheral blood samples were collected and bone marrow nucleated cells were counted. The reactive oxygen species (ROS) and colony forming unit granulocyte- macrophage (CFU-GM) were detected. Results　Compared to the control group, white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT) and platelet (PLT), bone marrow mononuclear cells (BMMNC) and CFU-GM of mice decreased significantly in irradiated group, the ROS level increased (P＜0.05). The WBC, RBC, HGB and PLT of the peripheral blood cells significantly decreased in the thymoquinone group (P＜0.05). The WBC, BMMNC and CFU-GM were enhanced significantly in the thymoquinone group compared with those of irradiation group (P＜0.05). Conclusion　The thymoquinone shows protective effects on the radiation-induced hematopoietic damage by inhibiting ROS levels to improve the proliferation of bone marrow cells. Related Articles | Metrics

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Mechanism of ginsenoside Rg1 regulating TLR4/NF-κB signaling pathway against myocardial tissue injury in rats with severe pneumonia SUNZhi-xia, SUOHong-liang△, LIHua, LIZong-shang, SHIGuang-yuan 2021, 49 (3):  271-276.  doi: 10.11958/20202164 Abstract ( 704 )   PDF (604KB) ( 4934 )   Objective　To investigate the effect and mechanism of ginsenoside Rg1 on myocardial tissue injury in rats with severe pneumonia. Methods　A total of 40 rats were injected with Klebsiella pneumoniae solution into trachea to construct severe pneumonia model. The model rats were divided into model group and low, medium and high dose drug groups. Another 10 untreated rats were taken as control group. The rats in the low, medium and high dose drug groups were given ginsenoside Rg1 with 2.5, 5 and 10 mg/kg by gavage, while the rats in the model group and the control group were given the same amount of normal saline, once a day for 14 days. Hematoxylin eosin stain method was used to observe the pathological morphology of myocardial tissue in rats and integral statistics was performed. The left ventricular diastolic pressure (LVDP), left ventricular end-diastolic pressure (LVEDP), maximal descending rate of left ventricular pressure (-dP/dtmax) and maximal developing rate of left ventricular pressure (+dP/dtmax) of rats were measured by color Doppler ultrasound. The serum contents of B-type natriuretic peptide (BNP), cardiac troponin I (cTnI) and MB isoenzyme of creatine kinase (CK-MB) were detected by enzyme-linked immunosorbent assay (ELISA).The mRNA expression levels of interleukin (IL)-6, IL-1β and tumor necrosis factor-α (TNF-α) were detected by real-time fluorescence quantitative PCR (qPCR). The protein expression levels of apoptosis-related protein cleaved cysteiny l aspartate specific proteinase 3 (cleaved caspase-3) and inactive state (procaspase-3), Bcl-2 and Bcl-2 associated X protein (Bax), Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-κB (NF-κB) p65 and phosphorylated (p)-NF-κB p65 in myocardial tissue were detected by Western blot assay. Results　The myocardial tissue structure was normal in the control group, and the central muscle cells of the model group were disorderly arranged and swelled, and the muscle fibers were partially broken and accompanied with inflammatory cell infiltration. Compared with the model group, the morphological changes of myocardial cells were significantly improved in low, medium and high dose drug groups. Compared with those in the control group, the pathological score, LVEDP level, BNP, cTnI, CK-MB contents, IL-6, IL-1β, TNF-α mRNA levels, cleaved caspase-3/procaspase-3, Bax/Bcl-2 ratio and TLR4, MyD88, p-NF-κB p65 protein expression levels were significantly higher in the model group, while the levels of LVDP, -dP/dtmax and +dP/dtmax were significantly lower (P＜0.05). After intragastric administration of ginsenoside Rg1, the above changes induced by modeling were reversed in a dose-dependent manner (P＜0.05). Conclusion　Ginsenoside Rg1 can protect severe pneumonia rats from myocardial tissue injury by reducing inflammatory reaction and myocardial apoptosis, and its mechanism may be related to the inhibition of TLR4/NF-κB signaling pathway activation. Related Articles | Metrics

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Relationship between VCAM-1, hs-CRP and lipid metabolism in children with primary nephrotic syndrome LIUZhu-feng, WANGWen-hong△, ZHANGXuan, WEINing, SONGShao-na 2021, 49 (3):  277-280.  doi: 10.11958/20202745 Abstract ( 776 )   PDF (360KB) ( 4392 )   Objective　To understand the effect of lipid metabolism disorder on the function of vascular endothelial cells, microinflammatory state and coagulation function through the detection of VCAM-1 and hs-C-reactive protein (CRP)in children with primary nephrotic syndrome (PNS). Methods　Sixty-five children who met the PNS standard were selected as the case group, and 45 healthy children matched in gender and age were selected as the control group. The serum levels of VCAM-1 and hs-CRP were measured by enzyme-linked immunosorbent assay (ELISA) and immunoturbidimetry in PNS group and control group. The 24 hour urine protein quantity was measured by special protein analyzer in the two groups. The serum levels of albumin (ALB), triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), ApoA1, ApoB, lipoprotein a [(LP(a)], fibrinogen (Fb) and D-dimer were also measured by special protein analyzer. Pearson method was used to detect urinary protein, blood levels of ALB, TG, TC, LDL-C, HDL-C, ApoA1, ApoB, LP(a)， Fb, and D-dimer and their relationship with VCAM-1 and hs-CRP. Results　The levels of VCAM-1 and hs-CRP were significantly higher before treatment in the case group than those in the control group. The levels of VCAM-1 and hs-CRP were significantly lower after hormone treatment than those before treatment in the case group. The blood levels of VCAM-1 and hs-CRP were positively correlated with urinary protein, serum levels of TG, TC, LDL-C, ApoB, LP(a)， Fb and D-dimer (P＜0.05). There was a negative correlation between blood levels of VCAM-1 and serum levels of ALB and HDL-C (P＜0.05). Conclusion　The significant increase of VCAM-1 and hs-CRP levels in PNS indicates that there is endothelial cell injury and micro inflammation in PNS children at acute stage. The level of VCAM-1 and hs-CRP can predict and evaluate the micro inflammatory state, endothelial cell injury, hypercoagulable state and thrombosis of PNS. Related Articles | Metrics

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The predictive value of R2CHA2DS2-VASc score for the risk of acute heart failure after acute myocardial infarction LIXin-hang, LIYuan-yang, SUNXu-sen, QIXin, WEILi-ping△ 2021, 49 (3):  281-284.  doi: 10.11958/20202253 Abstract ( 593 )   PDF (384KB) ( 4746 )   Objective　To investigate the predictive value of R2CHA2DS2-VASc score in acute heart failure (AHF) after acute myocardial infarction (AMI). Methods　A total of 139 patients with AMI were enrolled in Tianjin Union Medical Center from January 2019 to December 2019. According to the presence of AHF after AMI in hospital, the patients were divided into the non-AHF group (n=94) and AHF group (n=45). General clinical data were used to calculate R2CHA2DS2-VASc score and to compare between the two groups. Spearman correlation analysis was performed to estimate the correlation between R2CHA2DS2-VASc score and Killip grading, and the correlation between R2CHA2DS2-VASc score and N-terminal pro-B type natriuretic peptide (NT-proBNP). Multivariate Logistic regression analysis were performed to estimate the contribution of clinical risk factors to trigger AHF after AMI. The value of the score in predicting AHF in patients with AMI was evaluated by ROC curve. Results　Proportion of diabetes mellitus history, proportion of ischemic stroke/transient ischemic attack (TIA) history, proportion of heart failure history, NT-proBNP, Gensini score and R2CHA2DS2-VASc score were significantly higher in AHF group than those in non-AHF group (P＜0.05). Spearman correlation analysis showed that R2CHA2DS2-VASc score was positively correlated with Killip grading and NT-proBNP (rs = 0.539 and 0.506 respectively, P＜0.05). Multivariate Logistic regression analysis suggested that R2CHA2DS2-VASc score ≥ 4 and Gensini score ≥60 were the independent risk factor for AHF after AMI. The ROC analysis revealed the area under the R2CHA2DS2-VASc score curve was 0.826 (95%CI: 0.751-0.900), and the sensitivity and specificity were 57.8% and 92.6%, respectively. Conclusion　R2CHA2DS2-VASc score has the value of predicting AHF after AMI. Related Articles | Metrics

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Correlation research between circulating microRNA-223 and platelet activity and prognosis in patients with acute coronary syndrome ZHANGYing-ying, GUOXu-kun, ZHENGJun-yi, LIUTing, ZHANGYing, MAJing, LIUYin 2021, 49 (3):  285-290.  doi: 10.11958/20201714 Abstract ( 804 )   PDF (487KB) ( 4889 )   Objective　To investigate the relationship between the expression level of plasma microRNA-223 (miR-223) and platelet reactivity as well as clinical prognosis in patients with acute coronary syndrome (ACS) after clopidogrel administration. Methods　A total of 208 ACS patients received percutaneous coronary intervention (PCI) were enrolled consecutively. All patients were treated with loading doses of clopidogrel combined with aspirin (DAPT) before intervention. Real-time PCR was used to quantify the expression level of plasma miR-223, and the platelet reactive index (PRI) was quantified through flow cytometry. Patients were divided into two groups according to the PRI median, low PRI group (PRI≤56.3%) and high PRI group (PRI>56.3%), with 104 cases in each group. Plasma miR-223 expression levels, basic clinical data and biochemical index were compared between two groups of patients. After 2-year follow-up, Cox proportional risk regression analysis was used to estimate the influencing factors of plasma miR-223 level, PRI, troponin I (TnI), B-type natriuretic peptide (BNP) and other parameters for major adverse cardiovascular events (MACE). The receiver operating characteristic (ROC) curve was plotted to evaluate the predictive ability of plasma miR-223 and PRI levels for MACE. Results　The level of circulating miR-223 was negatively correlated with PRI (rs=-0.420, P＜0.05), and miR-223 expression level was significantly higher in low PRI group compared with that of high PRI group [1.15 (0.58，1.80) vs.0.64 (0.26，1.08), Z=0.471, P＜0.05]. MACE occurred in 30 patients (14.4%), and the incidence of MACE was lower in low PRI group than that in high PRI group (P < 0.05). Cox proportional risk regression analysis showed that the plasma miR-223 level and PRI were independent risk factors for predicting MACE in ACS patients. The AUC of plasma miR-223 and PRI for predicting the occurrence of MACE in ACS patients was 0.700 (95%CI: 0.609-0.791, P＜0.05) and 0.710 (95%CI: 0.606-0.815, P＜0.05), respectively. Conclusion　The circulating miR-223 level is negatively correlated with PRI in ACS patients after taking clopidogrel. The circulating miR-223 level and PRI are independent factors for predicting MACE in ACS patients after PCI. Related Articles | Metrics

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The predictive value of mean platelet volume and lymphocyte ratio in patients with acute ischemic stroke treated with intravenous thrombolysis LUHong-xiang, YANGBo-feng, CHENGYing, KONGXiao-xiang△ 2021, 49 (3):  291-295.  doi: 10.11958/20202510 Abstract ( 736 )   PDF (459KB) ( 4519 )   Objective　To investigate the predictive value of peripheral blood mean platelet volume and lymphocyte ratio (MPVLR) in the prognosis of acute ischemic stroke (AIS) patients treated with recombinant tissue plasminogen activator (rtPA) intravenous thrombolysis. Methods　 A total of 341 patients with AIS were collected. According to the modified Rankin scale (mRS) at 90 days after the onset of AIS, patients were divided into good prognosis group (mRS＜3, n=198) and poor prognosis group (mRS≥3, n=143). The baseline data and laboratory test results were collected in the two groups of patients. The multivariate Logistic regression was used to analyze the factors affecting the poor prognosis of patients. The receiver operating characteristic (ROC) curve was draw to judge and evaluate the prediction threshold of lymphocyte, platelet count (PLT), MPV, MPVLR and PLR for the poor prognosis of AIS patients. Results　(1) The age, NIHSS score，MPV，MPVLR，platelet to lymphocyte ratio (PLR) were significantly higher in the poor prognosis group than those of the good prognosis group (P＜0.05). Data of the lymphocyte count and PLT were significantly lower in the poor prognosis group than those of the good prognosis group (P＜0.05). Multivariate Logistic regression analysis showed that age (OR=1.026, 95%CI: 1.004-1.049), the admission NIHSS score (OR=1.112, 95%CI: 1.057-1.170), MPVLR (OR=3.415, 95%CI: 2.187-5.332) were the independent risk factors for the prognosis of AIS patients at 90 days. (2) ROC analysis showed that MPVLR (0.776) was better for predicting the poor prognosis of AIS than lymphocyte count (0.676), PLT (0.646), MPV (0.659) and PLR (0.703). The best prediction cutoff value of MPVLR was 6.5. With 6.5 as the cutoff value. The age, admission NIHSS score, MPV and PLR were higher in the high MPVLR group (≥6.5, n=174) than those in the low MPVLR group (<6.5, n=167), and the counts of lymphocytes and PLT were lower in high MPVLR group than those of the low MPVLR group (P＜0.05). Conclusion　Peripheral MPVLR might be used as an effective predictor of prognosis in AIS patients treated with rtPA intravenous thrombolysis, which is helpful for risk stratification and individual treatment. Related Articles | Metrics

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The value of the ratio of neutrophil to lymphocyte in predicting glucocorticoid induced osteonecrosis in minimal change disease SHENYa, DONGYi-jun, CHENYa-zhuo, GAOJing-ge, SHENGXiao-xiao, XIAOJing, ZHAOZhan-zheng, CHENGGen-yang△ 2021, 49 (3):  295-300.  doi: 10.11958/20202617 Abstract ( 764 )   PDF (510KB) ( 5015 )   Objective　To investigate the value of the ratio of neutrophils to lymphocytes (NLR) in the prediction of glucocorticoid induced osteonecrosis in patients with minimal change disease (MCD). Methods　A total of 329 MCD patients diagnosed by percutaneous renal biopsy were retrospectively analyzed. According to receiver operating characteristic(ROC) curve, the best cutoff value of NLR for diagnosing steroid induced osteonecrosis in MCD was determined. The subjects were divided into low NLR group (NLR ≤ 3.321, n=262) and high NLR group (NLR > 3.321, n=67). The baseline clinical indicators, hormone dosage and time, combined use of calcium and active vitamin D3 were compared between the two groups. The correlation between NLR and steroid induced osteonecrosis in MCD patients was analyzed. The joint survival rate was compared by Kaplan Meier survival curve between the two groups. Multivariate Cox regression model was used to analyze the risk factors of steroid induced osteonecrosis in patients with MCD. Results　 The median follow-up time for all the patients was 38 months. The age, white blood cell count, monocyte count, C-reactive protein, erythrocyte sedimentation rate, fibrinogen, osteocalcin, β-CTX, blood urea nitrogen, serum creatinine and the proportion of osteonecrosis were significantly higher in the high NLR group than those in the low NLR group (P＜0.05). The eGFR was significantly lower in the high NLR group than that in the low NLR group (P＜0.05). Kaplan-Meier survival curve showed that the joint survival rate was significantly higher in the low NLR group than that of high NLR group (c2=10.130, P＜0.01). The 1-year, 3-year and 5-year joint survival rates were 82.9% vs. 93.5%, 76.3% vs. 88.9% and 65.2% vs. 85.6% in the high NLR group and the low NLR group, respectively. Multivariate Cox regression analysis demonstrated that the high NLR level was an independent risk factor for glucocorticoid induced osteonecrosis in MCD patients (HR=2.155, 95%CI=1.136-4.089, P＜0.05). Conclusion　There is a positive correlation between blood NLR level and the risk of glucocorticoid induced osteonecrosis in MCD patients, and NLR could be used as a valuable indicator to evaluate the risk of steroid induced osteonecrosis in MCD patients. Related Articles | Metrics

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Study on the effect of adenomyosis on the clinical outcomes of in vitro fertilization-embryo transfer based on propensity score matching method LIUMin, LUOHai-ning, ZHANGYin-feng, ZHANGBo-lun, YANGZe-xin, ZHANGYun-shan△ 2021, 49 (3):  301-304.  doi: 10.11958/20202454 Abstract ( 550 )   PDF (351KB) ( 4132 )   Objective　To explore the effect of adenomyosis on the clinical outcomes of in vitro fertilization-embryo transfer (IVF-ET) by using propensity score matching (PSM) method. Methods　A total of 740 women underwent IVF-ET were retrospectively analyzed. Among them, there were 134 patients with adenomyosis (adenomyosis group) and 606 patients with infertility due to fallopian tube factors (control group). Based on age, years of infertility, body mass index (BMI) and basic estrogen level, the two groups of patients were matched with 1∶1 propensity score matching (PSM). After matching, there were 96 cases in each of the two groups. The differences in baseline characteristics, fertility outcomes and pregnancy outcomes before and after matching were compared between the two groups. Results　Before and after PSM matching, the dosage of gonadotropin (Gn) was increased, and the daily estradiol (E2) level of human chorionic gonadotropin (HCG) was decreased in the adenomyosis group compared with those of the control group (P＜0.05). In terms of the outcomes of assisted pregnancy, the number of oocytes retrieved, the number of mature oocytes, the number of oocytes normally fertilised, the number of available embryos and the number of frozen embryos were significantly lower in the adenomyosis group than those in the control group (P＜0.05). Conclusion　Adenomyosis is related to the adverse pregnancy outcomes of IVF-ET. Related Articles | Metrics

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Study on the correlation between the size of parathyroid adenoma and the function of parathyroid and thyroid WANGKang, XUWei-guo△, DONGHong-chao, JIAChun-liang, DINGMei, DONGLi-ru, SIRui-fen 2021, 49 (3):  305-309.  doi: 10.11958/20202103 Abstract ( 680 )   PDF (401KB) ( 4345 )   Objective　To analyze the situation of parathyroid adenoma (PTA) with thyroid disease, and discuss the relationship between the size of PTA and the function of parathyroid and thyroid. Methods One hundred patients with PTA admitted to the Affiliated Hospital of North China University of Technology were selected in this study. Patients were divided into two groups according to whether they were combined with thyroid diseases， including patients with thyroid disease group (n=55) and those without thyroid disease group (n=45). The clinical data of the patients were collected, and the correlation between preoperative indexes: age, course of the disease, alkaline phosphatase (ALP), corrected serum calcium, phosphorus, creatinine, total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), free thyroxine (FT4), thyroid-stimulating hormone (TSH), parathyroid hormone (PTH) and the size of PTA were analyzed. Logistic regression was used to analyze the risk factors of large adenoma, and receiver operating characteristic (ROC) curve was drawn to analyze the clinical value of preoperative indicators in predicting the larger adenoma. Results　Compared with patients without thyroid disease, a shorter adenoma course was found in the patients with thyroid disease (P＜0.05). There were positive correlations between the size of PTA and corrected serum calcium, PTH, serum creatinine, and course of disease. There was a negative correlation between the size of PTA and FT4 (P＜0.01). Preoperative PTH was positively correlated with corrected blood calcium, ALP, creatinine levels and disease course, and negatively correlated with blood phosphorus, TT4 and FT4 (P＜0.01). Preoperative TT4 level was negatively correlated with corrected blood calcium, ALP, creatinine levels and disease course, and positively correlated with blood phosphorus and FT4 levels (P＜0.05). Preoperative FT4 level was negatively correlated with adjusted blood calcium, FT3 level and disease course (P＜0.01). Logistic regression analysis found that preoperative PTH and disease course were risk factors for predicting large adenomas. The ROC curve suggested that the combined detection of PTH and disease course before surgery can further improve the sensitivity of predicting large adenomas. Conclusion　The size of PTA is positively correlated with preoperative PTH level. The combined consideration of preoperative PTH and course of disease could be a predictor of larger PTA, suggesting that hyperparathyroidism may inhibit thyroid function. Related Articles | Metrics

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The comparison of the risk stratification and diagnostic strategy of stable chest pain guided by NICE and ESC guidelines by using coronary CT angiography ZHUHui-jie, ZHANGYing△, LIUYu-jie, ZHOUJia 2021, 49 (3):  310-314.  doi: 10.11958/20202381 Abstract ( 752 )   PDF (414KB) ( 5258 )   Objective　In view of different risk stratification and diagnostic strategies for patients with stable chest pain, coronary computed tomographic angiography (CCTA) was used to verify and compare the advantages and disadvantages of the risk stratification and diagnostic strategies guided by the 2016 NICE and 2019 ESC guidelines. Methods　A total of 1 021 patients with stable chest pain who underwent CCTA were collected in Tianjin Chest Hospital from January 2017 to January 2020. According to the risk stratification strategy of NICE and ESC guidelines, the patients were divided into low risk group and high risk group. Coronary artery disease (CAD) was defined as stenosis≥50% in diameter at least one segment of the coronary artery by CCTA. The risk stratification and diagnosis strategies of the guidelines were verified and compared by using area under the receiver operating characteristic curve (AUC) and the net reclassification improvement (NRI). Results　Overall, 212 patients were diagnosed as CAD by coronary CCTA examination. Among the different risk stratification groups of ESC and NICE, patients in the high risk group were more likely to develop coronary heart disease than those of the low risk group (NICE: 25.62 % vs. 7.69%, χ2=41.866, P＜0.01; ESC: 45.06% vs. 3.47%, χ2=262.450, P＜0.01). The risk stratification strategy of ESC was improved compared with that of NICE, and which has better classification capabilities (NRI=38.54%, P＜0.01). The ESC guideline predicted that the AUC of coronary heart disease was 0.837 (95%CI: 0.813-0.860). The expected prevalence of coronary heart disease based on the ESC guideline greatly underestimated the observed prevalence (14.63% vs. 20.76%, χ2=13.349, P＜0.01), and this difference also existed in male patients (21.25% vs. 32.15%, χ2=13.580, P＜0.01). There was no significant difference in female patients (9.39% vs. 11.75%，χ2=1.561，P＞0.05). Conclusion　The ESC guideline for stable chest pain is better than that of NICE in the risk strategy of stable chest pain. The diagnostic strategy of ESC guideline has a good diagnostic value for coronary heart disease, but it underestimates the actual prevalence rate. Related Articles | Metrics

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The effect of preoperative oral multi-dimensional carbohydrates on painless colonoscopy in elderly patients TANCheng-wei, ZHUZhao-qiong△, DONGLiang, LIUDe-hang 2021, 49 (3):  315-319.  doi: 10.11958/20202737 Abstract ( 1003 )   PDF (390KB) ( 4819 )   Objective　To investigate the clinical application value of oral multi-dimensional carbohydrates 2 h before painless colonoscopy in elderly patients. Methods　A total of 120 elderly patients with painless colonoscopy were selected, and they were randomly divided into oral multi-dimensional carbohydrate group (energy mixture group), intravenous infusion of sodium potassium magnesium calcium and glucose injection group (infusion control group) and control group (blank control group). There were 40 cases in each group. The blank control group was prepared according to the traditional intestinal preparation, without any rehydration intervention. The energy mixture group was given 5 mL/kg of energy mixture 2 h before painless colonoscopy, and the maximum dosage was not more than 400 mL. The infusion control group was given 500 mL sodium potassium magnesium calcium glucose injection 2 h before painless colonoscopy. The colonoscopy operation time, propofol dosage, recovery time, postanesthesia care unit (PACU) stay time, vasoactive drug use, thirst, visual analogue scale (VAS) of hunger and fatigue, christensen postoperative fatigue score were recorded, and systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and heart rate (HR) were measured at the time points of entering the room (T0), induction (T1), entering the mirror (T2), withdrawing the mirror (T3) and entering the PACU (T4). After the examination, adverse reactions of the three groups were recorded. Results　There were no significant differences in colonoscopy operation time and propofol dosage between the three groups (P＞0.05). Compared with the blank control group,the recovery time and PACU stay time were significantly shorter in the energy mixture group and the infusion control group, and the utilization rate and repeated use rate of vasoactive drugs decreased (P＜0.05). At the time of T1, T2 and T4, HR values were significantly lower in energy mixture group and infusion control group than those in blank control group (P＜0.05). At the time of T2, values of SBP, DBP and MAP were higher in energy mixture group and infusion control group than those in blank control group (P＜0.05). At the time of leaving PACU, the scores of thirst and hunger were significantly lower in the energy mixture group than those in the infusion control group and the blank control group (P＜0.05). After the examination, compared with the blank control group, the incidence of severe hypotension and prolonged recovery time and the scores of fatigue out of room were significantly reduced in the energy mixture group and infusion control group (P＜0.05). Conclusion　Oral administration of multi-dimensional carbohydrates 2 h before colonoscopy in elderly patients can reduce the fluctuation of heart rate and blood pressure during the examination, shorten the recovery time, and reduce hunger and fatigue degree after operation. Related Articles | Metrics

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Construction and verification of a prediction nomogram for early mechanical ventilation in patients with severe pneumonia LIUKai-feng, ZHANGZhen△, ZHANGZheng-ping, WANGZhen-hua 2021, 49 (3):  320-324.  doi: 10.11958/20201075 Abstract ( 613 )   PDF (532KB) ( 4823 )   Objective　To explore and establish a clinical predictive model for patients with severe pneumonia. Methods　A total of 185 patients with severe pneumonia treated in our hospital were retrospectively analyzed. According to whether the patient received mechanical ventilation within 24 hours, they were divided into mechanical ventilation group (n=123) and non-mechanical ventilation group (n=62). Data of patient sex, age, blood gas analysis indicators such as arterial partial pressure of oxygen [p(O2)], arterial partial pressure of carbon dioxide [p(CO2)], alveolar-arterial partial pressure of oxygen [p(A-a)O2], oxygenation index (OI), and some laboratory findings at admission were analyzed. Multivariate Logistic regression was used to screen the risk factors affecting the need for mechanical ventilation in patients with severe pneumonia. A predictive model based on these selected indicators was constructed, and a nomogram was plotted. The predictive value of the model was evaluated through the receiver operating characteristic (ROC) curve and calibration curve. Results　Compared with the non-mechanical ventilation group, data of age, p(A-a)O2, APACHEⅡ score and p(CO2) were significantly higher in the mechanical ventilation group, while procalcitonin (PCT), central venous oxygen saturation (ScvO2), p(O2) and OI were significantly lower (P＜0.05). Logistic regression analysis showed that age, p(O2), p(CO2), p(A-a)O2 and OI were independent factors affecting whether the patients needed mechanical ventilation. The nomogram model constructed by the above five indicators showed a good discrimination (AUC=0.827, 95%CI: 0.785-0.898) and accuracy, which was better than the traditional p(O2)+ p(CO2)+OI model and OI model. Conclusion The nomogram model established based on age, p (O2), p (CO2), OI and p (A-a)O2 can accurately predict whether mechanical ventilation is required in the early stage of severe pneumonia patient. Related Articles | Metrics

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Study on Fourier transform mid-infrared spectroscopy in serum of breast cancer patients SHENJie, ZHULi-ying, ZHUKe-jing, DAILong-guang, XUYong-jie, XUWen, LIUXin-lei, LIXing, PANWei, △ 2021, 49 (3):  324-329.  doi: 10.11958/20202276 Abstract ( 639 )   PDF (853KB) ( 4615 )   Objective　To establish an effective method for distinguishing and identifying normal people and breast cancer patients based on Fourier transform mid-infrared spectroscopy technology. Methods　The serum samples of 86 female normal people and 85 female breast cancer patients were collected, and the spectra of the serum samples were drawn for the two groups of people. The principal component information of the two types of serum samples were extracted. The principal component score 2D and 3D scatter plots were drawn, and the scores of principal component 1-10 (PC1-PC10) were further calculated. Using the principle of discriminant analysis to establish a discriminant analysis model, and all samples were judged based on Mahalanobis distance. The performance index scores of models built under different spectrum preprocessing methods were calculated within the wave number range of 3 931-619 cm-1, and the best preprocessing method was selected to build the model. Results　There were significant differences in the peak intensities of the spectrum at wave numbers 3 363 cm-1, 2 360 cm-1, 1 641 cm-1, 1 552 cm-1, and 663 cm-1 between female normal population and female breast cancer patients (P＜0.05). The results of principal component analysis showed that there were significant differences in PC1-PC4 between two groups (P＜0.05), and there were no significant differences in PC5~PC10 between two groups (P>0.05). Compared with the normal group, the Mahalanobis distance to N in the breast cancer group is higher, and the Mahalanobis distance to C is lower (P＜0.05), and the positive judgment rate of the validation set of the built model was 100%. The model built without any processing on the spectrum was the best, and the performance index score was 94.1 points. Conclusion　Fourier transform mid-infrared spectroscopy can be used to identify and distinguish normal people and breast cancer patients, and it is expected to become a method for assisting breast cancer diagnosis. Related Articles | Metrics

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Research progress on the role of cartilage oligomeric matrix protein in human diseases JINLian-chi, LAIYong-jie△ 2021, 49 (3):  330-336.  doi: 10.11958/20202516 Abstract ( 1170 )   PDF (455KB) ( 4562 )   Cartilage oligomeric matrix protein (COMP) is a non-collagenous glycoprotein component of extracellular matrix (ECMs). COMP interacts with other ECMs, cell adhesive proteins, growth factors via its multi-functional domains. COMP is known to play a role in the development of miscellaneous human diseases. This paper reviews the basic structure and function of COMP and its role in the pathogenesis of joint and cardiovascular diseases, tissue fibrosis and cancer. Related Articles | Metrics