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null Abstract （2030）      PDF （290KB）（27812）       Knowledge map    Related Articles | Metrics

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Expression of ERG gene in tumor and its clinical research progress WANG Wen-jun, YU Yu, CUI Jiu-wei Tianjin Medical Journal    2019, 47 (2): 213-220.   DOI: 10.11958/20181058 Abstract （2149）      PDF （539KB）（8978）       Knowledge map    ETS-related gene (ERG) belongs to the E26 transformation-specific (EST) transcription factor family and plays an important role in the development and production of blood vessels, hematopoietic system and cartilage development. In recent years, more and more studies have found that ERG gene overexpression occurs in some tumors, thereby promoting tumor cell proliferation and tumor angiogenesis, and involving in the occurrence and development of tumors, such as prostate cancer (PCa), Ewing's sarcoma (EWS), leukemia, cartilage tumors. However, the specific mechanism of the action of ERG in these tumors is not yet fully clear. At present, the application of ERG in the diagnosis and prognosis of tumors has become a research hotspot. Some drugs that target ERG and its downstream signaling pathways and target genes are in clinical trials. This article reviews the expression, mechanism of action and clinical application of ERG gene in tumors. Related Articles | Metrics

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Research progress of Nrf2/ARE pathway regulating mechanism YI Xiaofang, TAN Chao Tianjin Med J    2015, 43 (5): 573-575.   DOI: 10.11958/j.issn.0253-9896.2015.05.032 Abstract （1489）      PDF （265KB）（8416）       Knowledge map    Abstract: Nuclear factor E2 related factor Nrf2 is a nuclear transcription factors involved in a variety of protein expres⁃ sion. As a center of oxidative stress regulation, it combines with antioxidant components (antioxidant responsive element, ARE) and activates downstream multiple anti-oxidation, anti-inflammatory and detoxifying enzyme protein expression. This signaling pathway is involved in the development of inflammation, tumor and other pathological process. This review de⁃ scribes the basic structure, biological effects and signaling pathways of Nrf2, summarizes the latest progress about mecha⁃ nisms of factors, which are involved in the positive and negative regulations of signal pathway, providing a new target for antiinflammatory, antioxidant, and antitumor biochemical treatment. Based on these, the paper also looks forward to applicating bioinformatics technology and providing better prospects for the development of target intervention. Related Articles | Metrics

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E6/E7 mRNA expression level in cervical lesions with high-risk HPV infection    2015, 43 (2): 186-188.   DOI: 10.11958/j.issn.0253-9896.2015.02.019 Abstract （2223）      PDF （287KB）（8148）       Knowledge map    Abstract： Objective To explore the clinical value of examining HPV E6 / E7 mRNA level in assessing cervical le⁃ sions infected with high- risk human papillomavirus (HR-HPV). Methods The cervical epithelial cells were collected from 265 patients with HR-HPV infection, including 100 cases of neoplasia free/ inflammation group (control group), 88 cas⁃ es of cervical intraepithelial neoplasia (CIN)Ⅰ, 33 cases of CINⅡ, 28 cases of CINⅢ and 16 cases of cervical carcinoma and the transcription of HPV E6 / E7 mRNA level was examined using branched DNA (b - DNA) technology. Results The positive rate HPV E6/E7 mRNA were higher in CIN Ⅱ (81.82%), CIN Ⅲ(89.29%) and cervical cancer group (100.00%) than tthat in control group (20.00%) and CINⅠ(35.23%) with significant difference, and there were no significant differences between other groups ; The positive rate and transcription level of HPV E6/E7 mRNA in HSIL (high grade squamous intraepi⁃ thelial lesion)and cancer group were significantly higher than normal, ASC(atypical squamous cell carcinoma) and LSIL（low grade squamous intraepithelial lesion) group (P < 0.05). Conclusion The transcription level of HPV E6 / E7 mRNA may re⁃ flect the activity of the virus and the progression of disease, and could be use as an effective indicator to screen high grade cervical pathological changes and a complementary method of cervical lesion screening. Related Articles | Metrics

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Research progress on the relationship between TMAO of new biomarkers and #br# cardiovascular diseases#br# WANG Jie, GAO Jing Tianjin Medical Journal    2020, 48 (12): 1244-1248.   DOI: 10.11958/20200333 Abstract （1471）      PDF （393KB）（7970）       Knowledge map    The relationship between gut microbiota and its metabolites and cardiovascular disease is a hot topic in cardiovascular research. It has made great progress in the basic and clinical research of gut microbiota in regulating cardiovascular physiology and disease progress. Some studies at home and abroad have shown that metabolite trimethylamine-N-oxide (TMAO), the gut microbiota, has become a key factor affecting the development of cardiovascular disease. In recent years, clinical studies on the relationship between TMAO and development and prognosis of cardiovascular diseases have also made some achievements. Plasma TMAO levels can be used as new biomarkers for the risk stratification, diagnosis and prognosis of cardiovascular diseases in the future, and the prediction of the occurrence of cardiovascular disease and the major cardiovascular events (MACE). This paper reviews the research progress of TMAO as a new biomarker and potential therapeutic target for cardiovascular diseases. Related Articles | Metrics

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The research progress of PD-1 channel inhibition in tumor treatment CHEN Lu, TAN Chao Tianjin Med J    2015, 43 (9): 1085-1088.   DOI: 10.11958/j.issn.0253-9896.2015.09.036 Abstract （1187）      PDF （305KB）（7754）       Knowledge map    Abstract: Programmed death1 (PD-1) is mainly expressed on the surface of activated T cell. The combination between PD-1 and its legends PD-L1/PD-L2 activate downstream signaling pathways and negatively regulate T cell activation. Ab⁃ normal increase expression of PD-L1 on tumor cell surface mediates the tumor immune escape. Inhibition of PD-1 signaling pathway contributes to antitumor effect of T cells. The development of this pathway inhibitors has become a hot spot for tu⁃ mor immunotherapy. This article expounds the progress about antitumor effects mediated by PD-1 pathway inhibition from experiments in vivo or in vitro and clinical development of PD-1 pathway inhibitors, providing a new target for cancer immu⁃ notherapy and theoretical and clinical basis for the clinical application of immunotherapy with traditional therapy methods. Reference | Related Articles | Metrics

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Review the connection between trimethylamine oxide and atherosclerosis WANG Weizhan1,2, TIAN Fengshi2△ Tianjin Med J    2015, 43 (4): 443-445.   DOI: 10.11958/j.issn.0253-9896.2015.04.030 Abstract （1408）      PDF （284KB）（7582）       Knowledge map    Abstract: Recent researches revealed that intestinal flora contribute to the development of metabolic syndrome, espe⁃ cially to the disorder of their targeted cardiovascular system. Blood cholesterol level is affected by the metabolism of intesti⁃ nal flora in cholesterol and bile acid. At the same time, the blood cholesterol level is the key player in development of athero⁃ sclerosis. This review mainly discussed the relationship between the intestinal flora dependent metabolite trimethylamine ox⁃ ide (Trimetlylamine oxide, TMAO) and atherosclerosis. Related Articles | Metrics

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Role of organoid in prostate cancer research DAI Liang，ZHANG Zhidong，TIAN Yao，JIANG Ning，NIU Yuanjie Tianjin Med J    2015, 43 (8): 946-950.   DOI: 10.11958/j.issn.0253-9896.2015.08.032 Abstract （1185）      PDF （335KB）（7563）       Knowledge map    As a new model of pre-cancer, organoid is essential for the basic understanding of tumor characteristic and effective tumor treatment. Organoids derived from prostate play an especially important role in the research of fundamental oncology and anticancer drug screen against prostate cancer. Prostate cancer cell lines and xenografts derived directly from primary human tumors are widely used now as models to study prostate cancer and have proven very valuable. But there are some caveats and shortcomes of these two models that have to be accounted for. Here we outline organoid as a third preclini⁃ cal cancer model which may potentially overcome the shortcomes of cancer cell lines and PDTX. This article aims to summa⁃ rizee recent progress of the role of organoid in prostate cancer research. Related Articles | Metrics

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Construction of Asxl2 gene knock out stable NIH3T3 cell line with CRISPR/Cas9n system FANG Jiaping1,2， ZHAO Xiujuan3， QI Yan4， WANG Xi3， WU Xudong3， LOU Jianshi1 Tianjin Med J    2015, 43 (10): 1104-1107.   DOI: 10.11958/j.issn.0253-9896.2015.10.005 Abstract （1511）      PDF （375KB）（7521）       Knowledge map    Abstract：Objective To knock out Asxl2 gene in murine embryonic fibroblast cell line NIH3T3 using CRISPR/Cas9n system. Methods A pair of sgRNAs which targeted exon 5 of Asxl2 gene were designed and subcloned into the pX462 vec⁃ tor. The recombined plasmids were verified by sequencing and transfected into NIH3T3 cell line. Single cells were isolated through serial dilutions, followed by an expansion period to obtain new monoclonal cell lines. The genomic DNA of the new monoclonal cell lines was extracted and a DNA fragment flanked the target site was amplified by genotyping PCR then se⁃ quenced. Lastly, western blotting were applied to confirm whether Asxl2 was successfully knocked out. Results The CRIS⁃ PR/Cas9n plasmids that targeted Asxl2 were successfully constructed. NIH3T3 cells were co-transfected with the two recom⁃ binant constructs. After puromycin selection, subclonal cell lines were obtained and one of them was validated by genotyping PCR-sequencing. Western blotting also confirmed that Asxl2 was completely depleted in the NIH3T3 cell line. Conclu⁃ sion CRISPR/Cas9n plasmids that targeted Asxl2 were successfully constructed therefore a Asxl2 knockout NIH3T3 stable cell line was established via this system. Related Articles | Metrics

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The characteristics of herpes simplex virus shedding in 142 cases of subclinical genital herpes ZHANG Jing1, HUANG Xi1, MENG Jian1, WANG Liping2, WEI Jiao1, JIANG Dongxiang1, CHENG Peihua1Δ Tianjin Med J    2016, 44 (11): 1384-1386.   DOI: 10.11958/20160696 Abstract （1548）      PDF （281KB）（7500）       Knowledge map    Objective To study the characteristics of shedding herpes simplex virus (HSV) in patients with subclinical genital herpes, and provide basis for clinical therapy. Methods A total of 142 patients with subclinical genital herpes were obtained in our hospital from June 2014 to June 2016. The fluorescent quantitation polymerase chain reaction was used to detect the HSV-DNA, and enzyme linked immunosorbent assay (ELISA) was applied to check the type of serum IgG. The effects of age, gender, antibody types, disease courses and occurrence frequency on the positive rate of HSV DNA shedding were analyzed. Results The positive rate of HSV-DNA in 142 patients was 49.3%, and females showed significantly higher HSV shedding rate than males (P < 0.05). There was no significant difference in HSV shedding rate between patients with serum HSV-Ⅰ IgG++HSV-Ⅱ IgG+ and patients with HSV-Ⅱ IgG+ alone, while both of which showed higher HSV shedding rate than those of patients with HSV- Ⅰ IgG + alone. Patients with short infection period (≤6 years) showed significantly higher HSV shedding rate than patients with longer infection period (>6 years). HSV shedding rate in patients with high frequent occurrence was found to be significantly higher than that in patients with low frequent occurrence (P < 0.05). In patients who showed short infection period (≤6 years), the HSV shedding rate was found significantly higher in frequent patients (recurrence ≥ 6 times/year) than that in few occurrence patients (recurrence < 6 times/year). There was no significant difference in HSV shedding rate in patients with recurrent occurrence when they went through more than 6 years’ HSV infection (P > 0.05). More patients with positive HSV- Ⅱ IgG were found in female than that in male (P < 0.05). Conclusion In patients with subclinical genital herpes, HSV shedding rate is closely related to gender, serum antibody type, disease course and recurrent occurrence. Related Articles | Metrics

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he effect of curcumenol on inflammatory factors in endometriosis model rats NIE Xiao-bo, MA Yi-kun, ZHAO Na, HU Bin, LIU Jiao, LIU Xiao-lan Tianjin Medical Journal    2019, 47 (9): 913-917.   DOI: 10.11958/20190727 Abstract （1070）      PDF （677KB）（7367）       Knowledge map    Objective To study the effect of curcumenol on the inflammatory factors in peritoneal fluid of rats with endometriosis (EMS), and to explore its therapeutic mechanism. Methods Adult female SD rats were used to prepare EMS model by autotransplantation. The EMS model rats were randomly divided into model group and curcumenol group (20 mg/ kg), and another sham operation group was set up, 10 rats in each group. Rats in each group were given the drug by intragastric administration once a day for four weeks. After the last administration, the rats were anesthetized, and samples of the abdominal fluid were collected. Pathological morphology of ectopic endometrium in rats was observed. The contents of monocyte chemotactic protein-1 (MCP-1), macrophage migration-inhibitory factors (MIF), tumor necrosis factor-alpha (TNF)-α, interleukin (IL)-1β and IL-6 in peritoneal fluid were detected. The TNF-α expression in endometrial tissue was detected by immunohistochemistry, and the fibroblast growth factor 2 (FGF-2) mRNA expression was detected by real-time PCR. Results Compared to the model group, the ectopic endometrium atrophy was found in curcumenol group, the contents of MCP-1, MIF, TNF - α, IL-1β, IL-6 in the peritoneal fluid of rats and the TNF - α expression, the FGF-2 mRNA expression in endometrial tissues were significantly decreased in curcumenol group (P＜0.05). Conclusion The curcumenol can inhibit the inflammatory reaction in the peritoneal microenvironment, which may be related to its treatment effect on EMS. Related Articles | Metrics

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Clinical analysis of 29 cases of fetal digestive tract malformation diagnosed prenatally by ultrasound ZHUO Na, DUAN Qing, ZHANG Hui， TIAN Jing，SUN Tong Tianjin Med J    2015, 43 (9): 1054-1055.   DOI: 10.11958/j.issn.0253-9896.2015.09.027 Abstract （1223）      PDF （268KB）（7292）       Knowledge map    Abstract: Objective To investigate the clinical significance of prenatal ultrasound examination in the diagnosis of fe⁃ tal digestive tract development. Methods Twenty-nine cases of congenital digestive tract malformation were examined in according to the different characteristics of their different fetal ultrasound images. Results There were 11 cases with non- magenblase or less magenblase (37.93%), 4 cases with combination of multiple malformations, and 9 cases with combination of amniotic fluid in the 29 cases. There were 7 cases (24.14%) with dilatation of intestine and intestinal vesicles, in which 3 with multiple malformations and 3 with polyhydramnios. There were 8 cases (27.58%) with double bubbles, in which 1 case with multiple malformations and 7 cases with amniotic fluid. Conclusion The prenatal ultrasound examination in 30 to 32 weeks of pregnancy is very valuable in diagnosis of fetal digestive tract development, which is worthy of clinical application. Related Articles | Metrics

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Value of ultrathin bronchoscopy, virtual bronchoscopic navigation, endobronchial ultrasonography with a guide sheath and rapid on-site evaluation in evaluation of bacterial infections in the peripheral third of the lung field LI Yajie, XIE Wei, ZHANG Peng, XUE Yanchao, FENG Jing△, CAO Jie△ Tianjin Med J    2016, 44 (1): 9-13.   DOI: 10.11958/20150162 Abstract （1367）      PDF （639KB）（7268）       Knowledge map    Abstract: Objective To evaluate the diagnostic yield and safety of transbronchial lung biopsy (TBLB) under virtual bronchoscopic navigation (Direct Path), endobronchial ultrasonography with a guide sheath (GS) and rapid on-site evaluation using an ultrathin bronchoscopy (UNRE) for bacterial infection located in the peripheral third of the lung field. Methods Ninety-seven patients with bacterial infection, which located in the peripheral third of the lung field on CT images, were ran⁃ domly assigned to UNRE (n=49) or non-UNRE (NUNRE, n=48) groups, who were treated in General Hospital of Tianjin Medical University between April 1, 2014 and March 31, 2015. The TBLB guided by UNRE was performed in two groups. The diagnostic yield, safety and complication rate were compared between two groups. Moreover, the differences of autofluo⁃ rescence intensity of alveolar macrophage in alveolar lavage fluid were compared between two groups of patients. Results The diagnostic yield was significantly higher in UNRE group than that of NUNRE group (81.6% vs 56.2%, χ2 =7.313, P < 0.01). The diagnostic yield was higher in UNRE group with bronchus sign compared to that of NUNRE. All patients had a mild bleeding at the time of biopsy. There were no hemoptysis, pneumothorax or other serious complications. The autofluores⁃ cence intensity of alveolar macrophage was different in different levels of infection in patients. Conclusion The procedure of UNRE has higher diagnostic rate and fewer complications. The careful selection of suitable cases can further improve the diagnostic accuracy. The autofluorescence intensity of alveolar macrophage in alveolar lavage fluid indicates the severity of infection in patients. Related Articles | Metrics

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The expressions of circled RNA CDR1as and miR-7 in epileptic patients and their relationships with abnormal EEG#br# HAN Yong-kai, LI Si-na, ZHANG Ping, LI Jing, WANG Xu-sheng, ZHANG Fan, DU Ai-ling, ZHANG Liu-sha, SONG Jing-gui△ Tianjin Medical Journal    2020, 48 (9): 871-874.   DOI: 10.11958/20201182 Abstract （872）      PDF （427KB）（7029）       Knowledge map    Abstract: Objective　To investigate the expressions of circular RNA cerebellar degeneration-related protein 1 antisense (CDR1as) and microRNA-7 (miR-7) in the plasma of epileptic patients and their relationships with abnormal EEG. Methods　A total of 87 epileptic patients treated and hospitalized in the Second Affiliated Hospital of Xinxiang Medical College from December 2016 to December 2019 were selected as the study objects (observation group). According to the electroencephalograph (EEG) results, patients were divided into normal group (n=6), mild abnormal group (n=18), moderate abnormal group (n=37) and severe abnormal group (n=26). In addition, 90 healthy people in the same period were selected as the control group. The plasma levels of CDR1as and miR-7 were detected by real-time fluorescent quantitative PCR (qPCR). The plasma levels of interleukin 2 (IL-2), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were detected by enzyme-linked immunosorbent assay (ELISA). Pearson's method was used to analyze the correlation between the levels of CDR1as, miR-7 and the levels of IL-2, TNF-α and IL-1β. Results　The levels of CDR1as, IL-2, TNF-α and IL-1β were significantly increased in the normal group, mild abnormal group, moderate abnormal group and severe abnormal group (P＜0.05), while the level of miR-7 was significantly decreased (P＜0.05). There was a positive correlation between the plasma levels of CDR1as and IL-2, TNF - α and IL-1 β in epileptic patients (P < 0.05). There was a negative correlation between the level of miR-7 and IL-2, TNF-α and IL-1β (P＜0.05). Conclusion　The plasma expressions of CDR1as and miR-7 are higher and lower in epileptic patients, respectively, and they are closely related to the levels of inflammatory factors and the abnormal degree of EEG, which may cause abnormal discharge of brain by influencing the  inflammatory response. Related Articles | Metrics

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The protective effect of alpinetin on chondrocyte damage induced by lipopolysaccharide DAI Wan-wu, HUANG Zu-quan, ZHANG Bo, DU Yong-jun, LI Xing-yan Tianjin Medical Journal    2020, 48 (12): 1137-1141.   DOI: 10.11958/20201496 Abstract （1342）      PDF （659KB）（6918）       Knowledge map    Objective To explore the protective effect of alpinetin on lipopolysaccharide (LPS)-induced rat chondrocytes damage and its mechanism. Methods The chondrocytes from the knee joints of suckling rats were isolated and cultured. They were divided into control group, LPS induction group (model group) and LPS induction plus alpinetin treatment group (alpinetin group). LPS were used to induce inflammation in the model group and the alpinetin group. The alpinetin group was treated with different concentrations of alpinetin after LPS induction. The CCK-8 method was used to detect chondrocyte activity, and the inflammation protection was observed by fluorescein diacetate/propidium iodide (FDA/PI) staining, real-time PCR (qPCR) and immunofluorescence staining effect. Results CCK-8 suggested that when the concentration of alpinetin was 5 mg/L, the absorbance of chondrocytes was the highest. FDA/PI staining results showed that the number of live cells was reduced in the model group compared with that the control group, while the number of dead cells were increased. Compared with the model group, the number of live cells were increased in the alpinetin group, while the number of dead cells decreased. qPCR results showed that the expression levels of interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS) , tumor necrosis factor (TNF-α) and human matrix metalloproteinase-13 (MMP-13) gene were decreased in the alpinetin group compared with those of the model group, while the expression level of type Ⅱ collagenase (Col2a1) was increased (P＜0.05). Immunofluorescence staining indicated that the expression level of IL-6 was significantly reduced in the alpinetin group compared with that of the model group. Conclusion Alpinetin can effectively protect the inflammatory damage of chondrocytes induced by LPS and promote the proliferation of chondrocytes, which provides a theoretical basis for the treatment of osteoarthritis. Related Articles | Metrics

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Clinical efficacy of inhaled N-acetylcysteine in the treatment of acute sinusitis LI Shu-hua, ZHAO Xiao-long, DENG Wei, JIANG Zhen-hua Tianjin Medical Journal    2020, 48 (12): 1201-1204.   DOI: 10.11958/20200835 Abstract （4035）      PDF （395KB）（6898）       Knowledge map    Objective　To observe the effect of inhaled N-acetylcysteine nebulization in patients with acute sinusitis, and its influence on the inflammatory factors and immune factors. Methods　A total of 164 patients with acute sinusitis were selected and divided into experimental group (n=82) and control group (n=82) by random number table. The control group was treated with conventional drugs, and the experimental group was treated with conventional drugs combined with N-acetylcysteine nebulized inhalation. Chemical luminescent immunoanalysis was used for the detection of serum levels of TNF-α, IL-6, IL-8 and IL-10 before and after treatment. The scattering immunoturbidimetric assay was used for the detection of immune factor levels (IgG, IgA and IgM). The visual analogue scale (VAS) scores, Lund-Kennedy scores in nasal endoscopy and treatment effect were also compared between the two groups of patients. Results　Compared with before treatment, the levels of TNF-α, IL-6, IL-8 and IL-10 in the two groups were significantly reduced, the levels of IgG, IgA, and IgM were increased, and the scores of VAS and Lund-Kennedy were significantly reduced. The changes of the above indicators in the group were more significant than those in the control group (P＜0.05). The total effective rate of treatment in the experimental group was significantly higher than that in the control group (P＜0.05). During the treatment period, all patients had no adverse reactions such as nausea, vomiting, rash, pruritus, and no complications such as intraorbital infection, optic neuritis, blindness, intracranial infection, etc. Conclusion　The inhalation of N-acetylcysteine in the treatment of acute rhinosinusitis is safe and effective, and which is worthy of clinical promotion and application. Related Articles | Metrics

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LncRNA-MALAT1 regulates the proliferation and apoptosis of colorectal cancer cells through the miR-142-3p/TEAD1 molecular axis ZHANG Zhi-cheng, YANG Qing-quan Tianjin Medical Journal    2020, 48 (12): 1146-1152.   DOI: 10.11958/20202143 Abstract （1064）      PDF （692KB）（6874）       Knowledge map    Objective　To explore the effect and related mechanisms of LncRNA-MALAT1 on the proliferation and apoptosis of colorectal cancer cells. Methods　The expressions of LncRNA-MALAT1 gene, miR-142-3p gene and TEAD1 protein in colorectal cancer cell lines and human normal colon epithelial cells were detected by Real-time PCR and Western blot experiments. Si-MALAT1 was transfected into HCT116 cells, and miR-142-3p inhibitor was co-transfected with si-MALAT1 into HCT116 cells on this basis. Real-time PCR and Western blot techniques were used to detect the expressions of LncRNA-MALAT1 gene, miR-142-3p gene, TEAD1 protein, Bax protein, Bcl-2 protein and Cyclin D1 protein in HCT116 cells. The level of cell proliferation was detected by CCK-8 assay, and the level of cell apoptosis in HCT116 cells was detected by flow cytometry. The dual luciferase experiment was used to detect the binding of LncRNA-MALAT1 and miR-142-3p, miR-142-3p and TEAD1 in HCT116 cells. Results　Compared with human normal colon epithelial cells, LncRNA-MALAT1 gene and TEAD1 protein were expressed at high levels and miR-142-3p gene was expressed at a low level in colorectal cancer cell lines. Silencing of LncRNA-MALAT1 could promote the expressions of miR-142-3p gene and Bax protein, inhibit the expressions of LncRNA-MALAT1 gene, Bcl-2 protein, Cyclin D1 protein and TEAD1 protein, inhibit the level of cell proliferation and promote the level of cell apoptosis in HCT116 cells. Co-silencing of miR-142-3p and LncRNA-MALAT1 could partially reverse the above regulatory effects. The binding of LncRNA-MALAT1 and miR-142-3p, miR-142-3p and TEAD1 in HCT-116 cells were detected by the dual luciferase experiment. Conclusion　LncRNA-MALAT1 could promote the expression of miR-142-3p target gene TEAD1, promote the capacity of proliferation in colorectal cancer cells, inhibit the level of apoptosis in colorectal cancer cells, and then promote the pathological process of colorectal cancer by binding to miR-142-3p. Related Articles | Metrics

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Application of dexmedetomidine in tourniquet lower limb surgery in elderly patients with #br# type 2 diabetes mellitus#br# CHEN Qian-xiu, LI Tian-mei, LIU Xing Tianjin Medical Journal    2020, 48 (12): 1210-1213.   DOI: 10.11958/20200716 Abstract （1030）      PDF （382KB）（6801）       Knowledge map    Objective To investigate the impact of dexmedetomidine (DEX) on serum neuron-specific enolase (NSE), oxidative stress, inflammatory response and postoperative cognitive function after lower limb tourniquet surgery in elderly patients with diabetes. Methods A total of 96 elderly patients with type 2 diabetes mellitus who underwent lower extremity surgery under selective general anesthesia tourniquet were randomly divided into DEX group and control group by using a random number table method, 48 cases in each group. After induction of general anesthesia, the DEX group was pumped with 0.5 μg/kg DEX within 15 min, followed by an infusion of 0.5 μg/(kg·h) until 30 min before the end of the operation. The control group was pumped with the same volume of normal saline. The serum levels of malonaldehyde (MDA), NSE，tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were detected by thiobarbituric acid and enzyme-linked immunosorbent assay (ELISA) before using the tourniquet, 30 min, 24 h and 72 h after releasing the tourniquet. The mini-mental state examination (MMSE) was used to evaluate cognitive function 1 d before operation, 1 d and 3 d after operation. The awake time, extubation time and adverse reactions were recorded in the two groups. Results Compared with before using the tourniquet, the level of NSE increased from 24 h after  releasing the tourniquet in both groups, while the levels of MDA, TNF-α and IL-1β increased from 30 min after releasing the tourniquet (P＜0.05). Compared with the control group, the level of NSE decreased from 24 h after tourniquet release in the DEX group, while the levels of MDA, TNF-α and IL-1β decreased from 30 min after tourniquet release (P＜0.05). Compared with 1 day before surgery, MMSE scores were decreased at the 1 d and 3 d after surgery in both groups (P＜0.05). Compared with the control group, MMSE scores increased at the 1 d and 3 d after surgery in DEX group (P＜0.05). There were no significant differences in wake time, extubation time and incidence of adverse reactions between the two groups. Conclusion Dexmedetomidine can reduce serum NSE, reduce oxidative stress and inflammation response, and improve postoperative cognitive function on lower limb tourniquet surgery in elderly patients with diabetes. Related Articles | Metrics

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Application of asymmetric z-plasty in repair of incomplete unilateral cleft lip null Abstract （2330）      PDF （63275KB）（6699）       Knowledge map    Objective：To explore the clinical application of asymmetric z-plasty in repair of congenital unilateral incomplete cleft lip. Methods：By individual design，65 patients with incomplete unilateral cleft lip were renewed lip height, repaired orbicular muscle of mouth and repaired nasal deformity. Results：The method was used in 65 patients, all cases were treated with primary healing. Followed up 6 months to 18 months，the ideal operative effects were obtained in all cases. Conclusion： the individual reconstruction of incomplete unilateral cleft lip is rational and simple，and is an ideal method for unilateral cleft lip repair. Related Articles | Metrics

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Effects of TRAP1 on proliferation and apoptosis of colorectal cancer cells LI Pin-yu, SHENG Wen-jie, ZHANG Yuan-yi, YANG Jian, ZHENG Pei-li, ZHANG Fei-fei Tianjin Medical Journal    2020, 48 (9): 813-817.   DOI: 10.11958/20193099 Abstract （1090）      PDF （556KB）（6671）       Knowledge map    Abstract: Objective　To investigate the expression of tumor necrosis factor receptor-associated protein 1 (TRAP1) in colorectal cancer and its effects on proliferation and apoptosis of colorectal cancer cells. Methods　The Oncomine database was used to analyze the expressions of TRAP1 in colorectal cancer tissues and normal intestinal mucosal tissues. Western blot assay was used to detect the expressions of TRAP1 in colorectal cancer tissues and their paired paracancerous mucosa tissues, LOVO, HT29, HCT116, RKO, SW480 and NCM460 cells. CCK-8 and plate cloning assay were used to detect the effects of TRAP1 on the proliferation of colorectal cancer cells. Flow cytometry was used to detect the effects of TRAP1 on cell cycle and apotosis. The effects of TRAP1 on cell cycle-associated protein, apoptosis-related protein and PI3K/AKT pathway markers were detected by Western blot assay. Results　Bioinformatics analysis showed that TRAP1 was highly expressed in colorectal cancer. Western blot results showed that the protein expression levels of TRAP1 were higher in colorectal cancer cells and tissues than those in normal tissues and cells (P＜0.05). Interfering TRAP1 expression with siRNA significantly inhibited cell proliferation and clonality (P＜0.05), and cells were arrested in G0/G1 phase, which increased apoptosis (P＜0.01). The expression of cell cycle-associated protein CyclinD1 was decreased, the expression of apoptosis-related protein Cleaved-Casp3 was increased and the expressions of p-PI3K and p-AKT were decreased (P＜0.05). Conclusion　TRAP1 is highly expressed in colorectal cancer. Interfering the expression of TRAP1 can inhibit the proliferation of colorectal cancer cells and promote apoptosis by inhibiting the activation of PI3K/AKT pathway. Related Articles | Metrics