天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (11): 1140-1145.doi: 10.11958/20190675

• 实验研究 • 上一篇    下一篇

甲状旁腺激素对老年大鼠种植体骨结合的影响及机制研究

许东亮，彭朝晖   

  1. 1河南省人民医院、郑州大学人民医院、河南大学人民医院口腔科（邮编450003）；2平煤集团总医院口腔科
  • 收稿日期:2019-03-12 修回日期:2019-08-14 出版日期:2019-11-15 发布日期:2019-12-17
  • 通讯作者: 许东亮 E-mail:dongliangd221@163.com

The effect and mechanism of parathyroid hormone on implant osseointegration in aged rats

XU Dong-liang,PENG Zhao-hui   

  1. 1 Department of Stomatology, Henan Provincial People's Hospital, Zhengzhou University People’s Hospital, Henan University People’s Hospital, Zhengzhou 450003, China; 2 Department of Stomatology, General Hospital of Pingmei Group
  • Received:2019-03-12 Revised:2019-08-14 Published:2019-11-15 Online:2019-12-17
  • Contact: Dongliang XU E-mail:dongliangd221@163.com

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摘要： 目的 探讨甲状旁腺激素对老年大鼠种植体骨结合的促进作用及机制。方法 将48只22月龄老年大鼠根据随机数字表法分为老年组和甲状旁腺激素组，每组24只，将24只12月龄成年大鼠作为对照组。3组大鼠左侧胫骨植入种植钛钉，甲状旁腺激素组大鼠腹腔注射甲状旁腺激素（20μg/kg），隔日1次，对照组和老年组大鼠腹腔注射等量生理盐水，共8周。采用双能X线骨密度测量仪测量上颌骨和胫骨骨密度。种植术后8周，测量种植体骨结合率（BIC）、种植体周围松质骨区骨量（TA）、骨皮质厚度（TCB）、骨小梁平均宽度（TW）、结合骨板宽度（CBLW）。采用实时定量聚合酶链反应（qPCR）种植体周围骨组织中碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨钙素（OCN）、白细胞介素-6（IL-6）mRNA水平。结果 种植术前，与对照组比较，老年组和甲状旁腺激素组大鼠上颌骨和胫骨骨密度均降低（P＜0.05）。种植术后8周，与老年组比较，甲状旁腺激素组大鼠上颌骨和胫骨骨密度升高（P＜0.05），BIC、TA、TCB、TW、CBLW值均升高（P＜0.05），种植体周围骨组织ALP、OPN、OCN mRNA水平升高（P＜0.05），IL-6 mRNA水平降低（P＜0.05）。对照组和甲状旁腺激素组大鼠种植体骨结合较好；老年组大鼠种植体骨结合差。结论 老年大鼠种植体骨结合差，甲状旁腺激素可促进老年大鼠种植体骨结合。

关键词: 牙种植, 骨内, 甲状旁腺素, 骨密度, 大鼠, Sprague-Dawley, 老年, 种植体钛钉

Abstract: Objective To investigate the promoting effect of parathyroid hormone on implant osseointegration in aged rats and its mechanism. Methods Forty-eight aged rats (22-month-old) were divided into the elderly group and the parathyroid hormone group accorded to the random number method, with 24 rats in each group. Twenty-four adult rats (12-month-old) were used as control group. Three groups of rats were implanted with titanium nails in the left tibia. Rats in the parathyroid hormone group were injected intraperitoneally with parathyroid hormone (20 μg/kg, qod), and rats in the control group and the elderly group were intraperitoneally injected with the same amount of normal saline for 8 weeks. The maxillary and tibia bone mineral densities were measured by dual-energy X-ray absorptiometry. Eight weeks after implantation, the bone-to-implant contact ration (BIC), trabercular area (TA), thickness of cortical bone (TCB), average trabecular width (TW) and combined bone width (CBLW) were measured. The alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN) and interleukin-6 (IL-6) mRNA levels in bone tissues around the implant were detected by real-time quantitative polymerase chain reaction (qPCR). Results Before implantation, compared with the control group, the bone mineral densities of the maxilla and tibia were decreased in the elderly group and the parathyroid hormone group (P＜0.05). After 8-week implantation, compared with the elderly group, the bone densities of maxillary and tibia in the parathyroid hormone group were increased (P＜0.05), the values of BIC, TA, TCB, TW and CBLW were increased (P＜0.05), the levels of ALP,OPN and OCN mRNA in the bone tissue around the implant were increased (P＜0.05), and the IL-6 mRNA level was decreased (P＜0.05). In the control group and parathyroid hormone group, the implant bone was well combined. In the elderly group, the implant bone was poorly coupled. Conclusion The bone density of aged rats is reduced, which affects the osseointegration of implants. Parathyroid hormone can increase bone mineral density in old rats and promote bone remodeling.

Key words: dental implantation, endosseous, parathyroid hormone, bone density, rats, Sprague-Dawley, old age, implant titanium nail