天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 188-192.doi: 10.11958/58360

• 实验研究 • 上一篇    下一篇

槲皮素对痛风性关节炎大鼠的治疗作用及肾功能的影响

黄敬群 1， 张军勇 2， 李伟中 3， 张亮 1， 高颖 1， 李光 1   

  1. 1中国人民解放军第252医院制剂中心 （邮编071000）， 2院部， 3检验科
  • 收稿日期:2015-02-02 修回日期:2015-09-19 出版日期:2016-02-15 发布日期:2016-02-15
  • 基金资助:
    中国博士后科学基金项目(2012M512105)

The therapeutic effect of quercetin on gouty arthritis and its influence in renal function in rats

HUANG Jingqun1, ZHANG Junyong 2, LI Weizhong 3, ZHANG Liang 1, GAO Ying 1, LI Guang 1   

  1. 1 Center of Praeparatum, 2 Command Institution, 3 Department of Laboratory Medicine, NO. 252 Hospital of PLA, Baoding, Hebei 071000, China
  • Received:2015-02-02 Revised:2015-09-19 Published:2016-02-15 Online:2016-02-15
  • Supported by:
    Chinese Postdoctoral Science Foundation

PDF (PC)

4574

摘要： 目的 探讨槲皮素对急性痛风性关节炎大鼠的治疗作用及其对肾功能的影响。方法 70 只雄性 SD 大鼠随机均分为空白对照组， 模型组， 秋水仙碱组（0.5 mg/kg）， 别嘌醇组（20 mg/kg）， 槲皮素低、 中、 高剂量组（100、 200、 400 mg/kg）。连续灌胃给药 7 d， 第 5 天采用微晶尿酸钠和次黄嘌呤法制备大鼠急性痛风性关节炎模型。造模前和造模后 2、 6、 12、 24 和 48 h 用缚线法测取右后肢小腿踝关节同一部位周径， 计算肿胀率； 采用比色法、 酶联免疫吸附法等测定大鼠血清中尿酸(UA)， β2-微球蛋白(β2-MG)， 胱素 C (Cys-C)， 尿素(Urea)， 肌酐(Cr)含量。脱颈处死大鼠， 取肾脏称质量， 计算肾脏指数。结果 槲皮素、 秋水仙碱、 别嘌醇能够显著抑制痛风性关节炎大鼠踝关节肿胀率； 造模后 8 h， 秋水仙碱、 槲皮素组血清 UA 水平均高于空白对照组和别嘌醇组， 别嘌醇组低于空白对照组； 48 h 后， 除别嘌醇组血清 UA 水平明显低于其余组外， 其余组间差异均无统计学意义。各组比较， 空白对照组β2-MG、 Cys-C 水平最低， 别嘌醇组 Urea、 Cr 水平及肾脏指数均最高 （均 P＜0.05）。结论 槲皮素对急性痛风性关节炎大鼠有显著的抗炎作用， 但造模可能导致肾功能不同程度的损害。槲皮素对轻度肾损伤未见明显的保护作用， 别嘌醇给药后加重肾功能损伤。

关键词: 痛风性关节炎, 高尿酸血症, 槲皮素, 秋水仙碱, 别嘌醇, 肾功能

Abstract: Objective To investigate the therapeutic effect of quercetin on acute gouty arthritis and its influence in renal function in rats. Methods Seventy male Sprague-Dawley rats were randomly divided into normal control group, model group, colchicine group (0.5 mg/kg), allopurinol group (20 mg/kg), quercetin 100, 200 and 400 mg/kg groups (n=10 for each group). Rats were administered various drugs by oral gavage once a day for seven consecutive days throughout the experiment. On the fifth day, the animal model of acute arthritis was set up by giving monosodium urate crystal combined with hypo⁃ xanthine. The inflammatory reaction was detected by measuring the circumference of right hind leg anklejoint with a tie line method at 0, 2, 6, 12, 24 and 48 h. The swelling ratio was calculated. The serum levels of uric acid (UA), β2-microglobulin (β2-MG), cystatin C (Cys-C), urea nitrogen (Urea) and creatinine (Cr) were detected by colorimetry and enzyme-linked immunosorbent assay.Rats were sacrificed at the end of experiment, and the kidney was weighed and the renal index was calcu⁃ lated. Results Treatment with quercetin, colchicine or allopurinol can significantly attenuate swelling rate in rats of acute gouty arthritis. The serum levels of UA were significantly higher in colchicine group and quercetin group than those of nor⁃ mal control group and allopurinol group. The serum levels of UA was significantly lower in allopurinol group than that of normal control group. After 48-h modeling, there was no significant difference in serum UA level between seven groups except allopurinol group. The levels of β2-MG and Cys-C were the lowest in normal control group than those of other groups. The serum levels of Urea and Cr and renal index were the highest in allopurinol group compared with those of other groups (P < 0.05). Conclusion Quercetin shows a significant effect of anti-inflammatory on acute gouty arthritis in rats. The model es⁃ tablishment may lead to different degrees of renal damage. Quercetin has no protective effect against renal injury, and allopurinol aggravates kidney injury.

Key words: gouty arthritis, hyperuricemia, quercetin, colchicines, allopurinol, renal function