天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 185-187.doi: 10.11958/59129

• 实验研究 • 上一篇    下一篇

补肾和脉方治疗动脉粥样硬化炎症的效果及机制探讨

Abdulai Fallah Tengbeh1， 郝青青 1,2， 陈旭 1， 曹新冉 1， 王莹 1， 徐飞飞 1， 董波 1,2△， 杨传华 3   

  1. 1山东大学附属省立医院心内科 （邮编250021）； 2山西医科大学汾阳学院病理生理学教研室； 3山东中医药大学附属医院心内科
  • 收稿日期:2015-06-23 修回日期:2015-09-19 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: △通讯作者 E-mail： dongbo1@medmail.com.cn E-mail:caoxinran_1010@126.com
  • 基金资助:
    国家中医药管理局资助课题 （JDZX2012113）

Therapeutic effects of Bu-shen-he-mai-fang (HMF) on inflammation in atherosclerosis

Abdulai Fallah Tengbeh1， HAO Qingqing1,2， CHEN Xu1， CAO Xinran1， WANG Ying1， XU Feifei1，#br# DONG Bo1,2△， YANG Chuanhua3#br#   

  1. 1 Department of Cardiology, Shandong Provincial Hospital Affiliated to Shandong University, Ji′nan 250021,China;2 Department of Pathophysiology， Fenyang College Shanxi Medical University; 3 Department of Cardiology，Affiliated Hospital to Shandong University of Traditional Chinese Medicine
  • Received:2015-06-23 Revised:2015-09-19 Published:2016-02-15 Online:2016-02-15
  • Contact: △Corresponding Author E-mail: dongbo1@medmail.com.cn E-mail:caoxinran_1010@126.com

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摘要： 目的 研究补肾和脉方（HMF）对载脂蛋白 E 基因敲除（ApoE-/-）小鼠动脉粥样硬化（AS）的治疗效果及可能机制。方法 将 24 只 ApoE-/-小鼠随机分为高脂组、 HMF 组和阿托伐他汀组， 每组 8 只。高脂组给予高脂饲料（0.25%胆固醇和 15%可可脂） 喂养， HMF 组给予高脂饲料+HMF[1.37 g/(kg·d)]喂养， 阿托伐他汀组给予高脂饲料+阿托伐他汀[5 mg/(kg·d)]喂养。8 周后， 戊巴比妥钠（0.8%， 0.05 mL/10 g）腹腔注射麻醉， 取小鼠胸主动脉切片， 行常规 HE 染色观察胸主动脉斑块大小； 行免疫组化染色检测巨噬细胞 CD68、 肿瘤坏死因子 （TNF） -α、 白细胞介素 （IL） -1β 和 IL-8 的表达水平。并采用日本全自动生化分析仪测量小鼠总胆固醇 （TC） 和总三酰甘油 （TG） 水平。结果 HMF 组胸主动脉斑块大小， 胸主动脉斑块内 CD68、 TNF-α、 IL-1β和 IL-8 的表达水平， TC、 TG 水平均明显低于高脂组， 高于阿托伐他汀组 （均 P＜0.05）。结论 HMF 具有抗动脉硬化作用， 可抑制 AS 进展， 其机制可能与降低血脂， 减轻斑块炎症细胞浸润， 减少炎症因子分泌有关。

关键词: 动脉粥样硬化, 炎症, 肿瘤坏死因子α, 白细胞介素 1β, 白细胞介素 8, 补肾和脉方

Abstract: Objective To observe the therapeutic effect and mechanism of Bu-shen-he-mai-fang (HMF) on experi⁃ mental atherosclerosis (AS) in apolipoprotein-E knockout（ApoE-/-）mice. Methods Twenty-four male ApoE-/- mice were randomly divided into three groups including high-fat group (0.25% cholesterol and 15% cocoa butter), HMF group [(highfat diet + HMF decoction 1.37 g/(kg·d)] and atrovastatin group [(high-fat diet + atrovastatin 5 mg/(kg·d)], 8 mice for each group. The serum level of lipid was evaluated by a kit. The extent of AS was evaluated by HE staining. The macrophage infiltration and expression of tumor necrosis factor-α(TNF-α), interleukin-1β (IL-1β) and interleukin-8 (IL-8) were evaluated by immunohistochemical staining. Results The serum level of lipid was lower in HMF group and atrovastatin group than that in high-fat group (P＜0.05). The degree of AS was significantly lower in HMF group and atrovastatin group than that in high-fat group. The macrophage infiltration, TNF-α, IL-1β and IL-8 expressions were significantly lower in HMF group and atrovastatin group than those in high-fat group (P＜0.05). Conclusion The results suggest that HMF significantly inhibits early atherosclerotic lesions by lowering serum lipid level and inhibiting inflammatory response.

Key words: atherosclerosis, inflammation, tumor necrosis factor-alpha, interleukin- 1beta, interleukin-8, Bu-shenhe-mai-fang