天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (1): 53-55.doi: 10.11958/59123

• 实验研究 • 上一篇    下一篇

同型半胱氨酸对大鼠局灶性脑缺血后血管再生的影响

苟芸， 黄国伟， 赵亚倩， 陈爽， 张绪梅△   

  1. 天津医科大学公共卫生学院营养与食品卫生学系 （邮编 300070）
  • 收稿日期:2015-06-19 修回日期:2015-07-25 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: △通讯作者 E-mail： zhangxumei@tijmu.edu.cn E-mail:zhangxumei@tijmu.edu.cn
  • 作者简介:苟芸 （1990）， 女， 硕士在读， 主要从事营养与神经退行性疾病研究
  • 基金资助:
    基金项目： 国家自然科学基金资助项目 （81373003）； 中国博士后科学基金 （2014M550148）

Effects of homocysteine on post-cerebral ischemic angiogenesis in rats

GOU Yun, HUANG Guowei, ZHAO Yaqian, CHEN Shuang, ZHANG Xumei △   

  1. Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China
  • Received:2015-06-19 Revised:2015-07-25 Published:2016-01-15 Online:2016-01-15
  • Contact: △Corresponding Author E-mail： zhangxumei@tijmu.edu.cn E-mail:zhangxumei@tijmu.edu.cn

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摘要： 摘要： 目的 探讨同型半胱氨酸 （Hcy） 对大鼠局灶性脑缺血后梗死灶周围区血管再生的影响， 为明确血管再生的抑制因素， 促进大脑功能恢复奠定临床基础。方法 将 36 只清洁级 （SD） 雄性大鼠随机分为 3 组： 假手术组 （SO 组）、 大脑中动脉闭塞 （MCAO） 模型组 （MCAO 组）、 MCAO+Hcy 组， 每组 12 只， SO 组和 MCAO 组腹腔注射生理盐水 5 mL/ （kg·d）， MCAO+Hcy 组腹腔注射 2%Hcy 溶液 5 mL/ （kg·d）， 预干预 7 d 后采用线栓法制作 MCAO 模型， 于术后第 7 天处死取材， 取材前 3 d 通过腹腔连续注射 5-溴脱氧尿嘧啶核苷 （BrdU）。通过高效液相色谱 （HLPC） 法检测大鼠血清中 Hcy 浓度， TTC 染色观察脑组织梗死灶区面积大小， 采用免疫荧光染色法检测梗死侧丘脑 BrdU+ /lamini + 细胞数目。结果 MCAO+Hcy 组大鼠血清 Hcy 浓度较 SO、 MCAO 组显著增高， 脑组织梗死面积较 MCAO 组大， 丘脑内 BrdU+ /laminin+ 细胞数目较 MCAO 组减少 （P < 0.05）。结论 体内 Hcy 浓度增高可增强脑梗死的损伤程度， 并且抑制缺血区周围部位的血管再生。

关键词: 脑梗死, 疾病模型,动物, 大鼠, Sprague-Dawley, 同型半胱氨酸, 血管再生

Abstract: Abstract: Objective To explore the role of homocysteine（Hcy）on angiogenesis at peri infarct region after focal cere⁃ bral ischemia in rats, to elucidate inhibitory factors of angiogenesis, and to establish a clinic foundation for clinical brain functional recovery. Methods Spragur-Dawley (SD) male rats (n=36) were randomly divided into three groups with 12 rats in each group including Sham Operation (SO) group, Middle cerebral artery occlusion (MCAO) group and MCAO+Hcy group. The rats in Sham and MCAO groups were intra-peritoneally injected with 5 mL/(kg·d) saline and rats in MCAO + Hcy group were injected with 2% 5 mL/(kg·d) Hey solution from the same route. MCAO was introduced by intraluminal filament meth⁃ od after 7 d Hcy intervention. Rat brains were harvested on the 7th day after MCAO. BrdU（50 mg/kg, as a marker of cell pro⁃ liferation）was intraperitoneally injected three days before the rats were killed. High performance liquid chromatography （HPLC）was used to measure serum Hcy concentration in rats. Brain infarction size was observed by TTC staining. Immuno⁃ fluorescence staining was used to detect the number of BrdU+ /laminin+ cells at the thalamus of infarction side. Results Se⁃ rum Hcy concentration significantly higher in MCAO + Hcy group than in SO and MCAO groups（P < 0.05） . Brain damage increased and the number of BrdU+ /laminin+ cells decreased in MCAO + Hcy group compared with those of MCAO group （P < 0.05） . Conclusion Increased Hcy concentration in rats lead to more severe damage of cerebral infarction as well as to inhibit the angiogenesis at surrounding ischemia area.

Key words: brain infarction, disease models, animal, rats, Sprague-Dawley, homocysteine, angiogenesis