天津医药

天津医药 ›› 2014, Vol. 42 ›› Issue (12): 1172-1175.doi: 10.3969/j.issn.0253-9896.2014.12.006

• 实验研究 • 上一篇    下一篇

Th17 和 Treg 细胞来源的儿茶酚胺在胶原诱导性关节炎中的作用

王小琴,彭聿平,刘展,邱一华   

  1. 南通大学医学院生理学系
  • 收稿日期:2014-09-10 修回日期:2014-09-29 出版日期:2014-12-15 发布日期:2014-12-15
  • 通讯作者: 王小琴 E-mail:wxq622197@ntu.edu.cn
  • 作者简介:
  • 基金资助:
    儿茶酚胺对类风湿性关节炎Treg/Th17失衡的作用及其受体信号转导机制;CD4+T细胞来源的儿茶酚胺在胶原诱导性关节炎中的作用

Role of Catecholamines Derived from Th17 and Treg Cells in Type Ⅱ Arthritis Induced byCollagen

,PENG Yuping , ,QIU Yihua   

  1. Department of Physiology, School of Medicine, Nantong University， Natong 226001, China
  • Received:2014-09-10 Revised:2014-09-29 Published:2014-12-15 Online:2014-12-15
  • About author:

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摘要：

【摘要】 目的 探讨胶原诱导性关节炎（CIA）疾病发生发展过程中 Th17 和 Treg 细胞来源的儿茶酚胺（CAs）的作用。 方法 随机将 18 只雄性 DBA/1 小鼠分为对照组、CIA 模型Ⅰ 组（35 d）和 CIA 模型Ⅱ 组（55 d）。 Ⅱ 型胶原乳剂尾根部注射制备 CIA 小鼠模型。 Real-time PCR 法检测淋巴结中 Th17 细胞的特异性转录因子 ROR-γt， 相关细胞因子 IL-17 和 IL-22， Treg 细胞的特异性转录因子 Foxp3， 相关细胞因子转化生长因子（TGF）-β以及酪氨酸羟化酶（TH）的 mRNA 表达。 免疫荧光组织化学染色法观察淋巴结中 ROR-γt 和 Foxp3 分别与 TH、囊泡单胺转运体-2 （VMAT-2）和单胺氧化酶（MAO）的共定位情况。 结果 与对照组相比， CIA 模型Ⅰ 组和模型Ⅱ 组的小鼠淋巴结中 ROR-γ、 IL-17、 TH 和 IL-22 的 mRNA 表达增加； Foxp3 和 TGF-β 的 mRNA 表达减少； 其中模型Ⅱ 组 IL-17 的 mRNA 表达与模型Ⅰ 组相比有所降低。 对照组和 CIA 模型Ⅰ 组及模型Ⅱ 组的小鼠淋巴结中存在 ROR-γt 和 Foxp3 分别与 TH、VMAT-2 和 MAO 的共定位细胞。 CIA 模型组小鼠淋巴结中 ROR-γt/TH、ROR-γt/VMAT-2 和 ROR-γt/ MAO 双阳性细胞数增加， 模型Ⅱ 组中的这些双阳性细胞数与模型Ⅰ 组相比有所减少。 结论 CIA 模型组小鼠淋巴结中 Th17 细胞合成 CAs 的能力增强， 这种 Th17 细胞来源的 CAs 的增加可能在 CIA 的发生发展过程有一定的抗炎作用。

关键词: 关节炎, 类风湿, 儿茶酚胺, T淋巴细胞, 调节性, 酪氨酸单氧化酶, Th17 细胞, 胶原诱导性关节炎

Abstract:

[Abstract] Objective To explore the role of Th17- and Treg-derived catecholamines during collagen-induced ar? thritis (CIA) progression. Methods Eighteen male DBA/1 mice were randomly divided into control group, CIA model group Ⅰ (day 35) and CIA model group Ⅱ (day 55). The CIA models were induced by type Ⅱ collagen (CⅡ ) injection from tails. mRNA expression of Th17 specific transcription factor include ROR-γt, cytokines, IL-17 IL-22, Treg specific transcription factor, Foxp3, cytokines, TGF-β and tyrosine hydroxylase (TH) in lymph nodes were examined by real-time PCR. Co-local? ization of ROR-γt or Foxp3, with TH, vesicular monoamine transporter-2 (VMAT-2) or monoamine oxidase (MAO) in lymph nodes were observed by immunofluorescence staining. Results In lymph nodes of mice in CIA Ⅰ group and CIA Ⅱ group, mRNA expression of ROR-γt, IL-17, TH and IL-22 were upregulated, while mRNA expression of Foxp3 and TGF-β ex? pression was downregulated compared to those expression in control group. The upregulated expression of IL-17 was signifi? cantly reduced in CIA Ⅱ group compared with that in CIA Ⅰ group. In the lymph nodes of both intact and CIA mice, co-lo? calization of ROR-γt or Foxp3 with TH, VMAT-2 or MAO was seen in some cells. The numbers of cells that are double-pos? itive of ROR-γt/TH， ROR-γt/VMAT-2 and ROR-γt/MAO IL-17 were increased in CIA groups compared to those in con? trol group. And they are significantly reduced in CIA Ⅱ group compared with those in CIA Ⅰ group. Conclusion The abili? ty to synthesize catecholamines in Th17 cells was increased in lymph node in mice from CIA groups compared to that in con? trol group. The increased catecholamines production from Th17 cells in lymph nodes may be involved in the anti-inflammato? ry progression in CIA.

Key words: arthritis, rheumatoid, catecholamine, T- lymphocytes, regulatory, tyrosine 3- monooxygenase, Th17cells, Catecholamines