天津医药

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淫羊藿总黄酮对环磷酰胺致免疫低下小鼠的免疫调节作用

王洪武1,贾亮亮2,张长城1,赵海霞1,姜美杰1,徐媛青1,曾晓1,何毓敏1,李玉洲1,袁丁1   

  1. 1. 三峡大学医学院
    2. 湖北省宜昌市三峡大学医学院
  • 收稿日期:2010-07-06 修回日期:2010-08-04 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 张长城

Immunoregulation Effects of Total Flavones of Epimedium on Immunosuppression Mice

  • Received:2010-07-06 Revised:2010-08-04 Published:2010-12-15 Online:2010-12-15

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摘要：

 目的:研究淫羊藿总黄酮(TFE)对环磷酰胺致免疫低下小鼠的免疫调节作用。方法:Balb/C小鼠40只,雌雄各半,随机分为空白组、模型组、TFE低、中、高剂量组,每组8只。空白组腹腔注射生理盐水,其余各组连续5d腹腔注射环磷酰胺建立免疫低下模型。空白组和模型组灌胃生理盐水,TFE低、中、高剂量组分别按200、400及800mg/kg剂量灌胃TFE。给药35d后测定各组体质量、脾质量、脾细胞绝对数量、相对数量、骨髓有核细胞数量及脾脏中CD3+、CD4+、CD8+、CD19+、NK细胞数量。结果:造模结束后,模型组脾细胞绝对数量、相对数量、骨髓有核细胞数量及脾脏中CD3+、CD4+、CD8+、CD19+、NK细胞的数量低于空白组;TFE低剂量组脾脏中CD3+、CD4+、CD8+、NK细胞及骨髓有核细胞数量高于模型组;TFE中剂量组和高剂量组的脾细胞绝对数量、骨髓有核细胞数量及脾脏中CD3+、CD8+、CD19+、NK细胞数量高于模型组;而且TFE高剂量组脾细胞相对数量和CD4+也高于模型组;差异均有统计学意义(P<0.01或P<0.05)。结论:TFE可显著增强环磷酰胺所致免疫低下小鼠的免疫功能,其部分机制在于增加脾细胞绝对数量和相对数量,调节脾脏中T细胞亚群、B淋巴细胞、NK细胞数量,并促进骨髓有核细胞的增殖。 

关键词: 淫羊藿总黄酮, 环磷酰胺, 免疫调节

Abstract: Abstract Objective:To investigate the immune regulatory function of total flavones of Epimedium on immunosuppression mice. Methods:The immunosuppression model was established by using cyclophosphamide(CY). The body weight and spleen weight were analysed. The absolute number of total splenocytes and bone marrow cells were determined by haemacytometer.The absolute number of CD4+T cells ,CD8+ T cells,B cells and NK cells in spleen was detected by flow cytometer. Results:Total Flavones of Epimedium significantly antagonist the loss in immune organ and body weight induced by cyclophosphamide, markedly restore the number of total splenocytes and bone marrow cells , improved the number of CD4+T cells ,CD8+ T cells,B+ cells and NK cells in spleen . Conclusion:Total Flavones of Epimedium could improve the immune function in immunodepressed mice induced by CY.

Key words: Total flavons of epimedium, Cyclophosphamide, Immunomodulation