天津医药

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男性高尿酸血症患者血浆代谢组学的研究

钟麒1,高玉霞2,常红1,王璇1,张美琳1,边姗姗1,黄国伟1   

  1. 1. 天津医科大学
    2. 天津医科大学总医院
  • 收稿日期:2010-12-16 修回日期:2011-03-10 出版日期:2011-08-15 发布日期:2011-08-15
  • 通讯作者: 钟麒

Analysis of Metabolites in Plasma of Male Hyperuricemia Patients by Metabonomics Methods

  • Received:2010-12-16 Revised:2011-03-10 Published:2011-08-15 Online:2011-08-15

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摘要： 目的：运用代谢组学方法研究男性高尿酸血症（hyperuricemia, HUA）患者血浆成分的变化，为高尿酸血症的病因研究、早期诊断提供依据。方法：运用氢核磁共振（1H-NMR）代谢组学方法分析高尿酸血症患者血浆中代谢物的变化，结合主成分分析和偏最小二乘判别分析法，分析病例组和对照组之间的代谢物差异。结果：与对照组相比，HUA组的饱和脂肪酸、N-乙酰糖蛋白、VLDL、乳酸、苏氨酸、乙醇的含量上升，卵磷脂、谷氨酰胺、葡萄糖的含量下降。结论：利用1H-NMR代谢组学法，可以分析得到HUA患者血浆代谢谱与正常对照组之间的区别。代谢组学结果提示HUA患者存在脂类代谢异常、糖酵解加剧、谷氨酰胺含量下降等问题。这可能与HUA的发病、病程转归有关，也可为HUA与代谢综合征各组分的关系研究提供线索与依据。

关键词: 代谢组学, 高尿酸血症, 核磁共振, 代谢物

Abstract: Objective：To analyse changes of metabolites in plasma of Male Hyperuricemia (HUA)patients by using metabonomics method. Method： The differences of metabolites between HUA group and health group were observed by NMR-based-metabonomics method,and the data was analyzed by principal components analysis (PCA) and orthogonal signal correction(OSC)-partial least squares (PLS) analysis. Results：Compared with health person ,HUA patients had a higher concentration of saturated fatty acid (SFA), N-Acetyl-Glucoprotein (Nac), VLDL,Lactate, threonine, ethanol and the concentration of lecithin, Glutamine,glucose is decreased. Conclusion： The differences between HUA group and health group can be observed by NMR-based-metabonomics method, which may contribute to the analysis of pathological mechanism and development of HUA disease,and it can also be used to analyze the relationship between HUA and Metabolic Syndrome(MS).

Key words: Metabonomics, Hyperuricemia, Nuclear Magnetic Resonance, Metabolites