天津医药

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个体化裸鼠荷人肺癌肿瘤模型的建立

王晓东1,孙大强2,李志1,白悦1   

  1. 1. 天津医科大学研究生院
    2. 天津市胸科医院胸外科
  • 收稿日期:2011-07-19 修回日期:2011-10-23 出版日期:2012-05-15 发布日期:2012-05-15
  • 通讯作者: 孙大强

The Establishment of Indivudal Human Lung Cancer Model by Xenotransplant Tumor Mass in Nude Mouse

  • Received:2011-07-19 Revised:2011-10-23 Published:2012-05-15 Online:2012-05-15

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摘要： 摘要 目的：建立人肺癌个体化可传代组织块动物模型。方法：组织块移植组将直径2mm的人肺癌新鲜标本癌组织块移植于BALB/C裸鼠背部皮下组织内，每例人肺癌新鲜标本均移植入5只裸鼠体内，成瘤成功者为第一代。取第一代移植瘤组织块以相同方法移植于BALB/C裸鼠背部皮下组织内，成功者为第二代。重复上述传代方法得到第三代移植瘤。将人肺癌细胞株A549、H1795分别移植于BACL/C裸鼠背部皮下组织内，成瘤达500mm3后取癌组织块。分别取实验组的原代人肺癌肿瘤及第一、二、三代移植瘤部分组织，及作为对照的细胞系移植瘤组织，固定于10%甲醛溶液中，石蜡包埋、切片、HE 染色，光镜下观察比较其形态异同。结果：组织块移植组瘤细胞不仅保留了人肺癌癌细胞的异型性，还保持原有排列结构；而作为对照的细胞系移植瘤细胞失去了原有的排列结构，仅保留了癌细胞的异型性。结论：利用不同患者的手术切除肺癌标本所建立的裸鼠荷人肺癌肿瘤模型具有个体化特征，能够为肺癌个体化治疗的研究提供有效工具。

关键词: 模型, 动物, 异种移植模型, 癌, 非小细胞肺

Abstract: Abstract Objective:To establish clinically relevant individuation nude mouse models of human non-small cell lung cancer,which can be stably passaged,so as to lay a foundation for the future individualized therapy study. Methods: Tumor implanting group: BALB/C nude mouse subcutaneously injected in the back with diameter 2mm tumor derived from human operative specimens were killed when the tumor mass grown to 800mm3.This is the first generation.Then,to establish the second generation, BALB/C nude mouse were injected in the back with diameter 2mm tumor derived from first generation tumor mass.the same method redone to gain the third generation tumor. Cell lines implanting group: BALB/C nude mouse subcutaneously injected in the back with 1×106 human lung cancer cell lines A549 or H1795 were killed when the tumor mass grown to 500mm3.The tissue of primary human lung cancer 、first 、second 、third generation implanting tumor and the cell lines implanting group tumor were formalin-fixed、cut sections、HE stained,and then compared with the morphology by light microscope.Results: The tumor cells of the tumor implanting group not only retains the atypia of cancer cells, but also keep original arrangement structure; The cell lines implanting group tumor cells lose their arrangement structure, keeping only the atypia of the cancer cells. Conclusion: Using different surgical excision of lung cancer established human lung cancer tumor nude mice-model which have individual characteristics,this model can be useful for the future individualized therapy study in vivo.

Key words: Models, Animal, Xenograft Model, Carcinoma, Non-Small-Cell Lung