天津医药

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天津地区人群中LOX-1基因3′-UTR多态性与冠心病的相关性研究

豆静,刘寅   

  1. 天津市胸科医院
  • 收稿日期:2011-10-13 修回日期:2012-02-09 出版日期:2012-09-15 发布日期:2012-09-15
  • 通讯作者: 豆静

Correlation between the polymorphism of 3′-UTR of Lectin-like Oxidized Low-density Lipoprotein Receptor-1 gene and coronary artery disease in Tianjin Population

  • Received:2011-10-13 Revised:2012-02-09 Published:2012-09-15 Online:2012-09-15

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摘要： 摘要 目的：研究天津地区人群中凝集素样氧化低密度脂蛋白受体-1（LOX-1）3′-非编码区（3′-UTR）188位点基因多态性情况，探讨LOX-1基因多态性与冠心病（CAD）的关系。方法：冠心病组170例，均经冠脉造影（CAG）确诊；对照组140例，均经CAG排查。应用聚合酶链反应-限制片段长度多态性（PCR-RFLP）法对总研究对象的LOX-1基因3′-UTR 188位点多态性进行分析，比较该基因多态性与冠心病的相关性。结果：冠心病组和对照组比较，LOX-1基因3′-UTR 188位点基因型频率和等位基因频率差异均无显著性统计学意义(均P>0.05)。结论：天津地区人群中LOX-1 3′-UTR 188位点基因多态性与冠心病的发病无明显相关性，不能作为冠心病的预测因素。

关键词: 凝集素类 受体 氧化LDL, 冠状动脉疾病, 冠状血管造影术, 等位基因, 基因频率, 多态性

Abstract: Abstract Objective: To investigate the polymorphism of 3′-UTR 188 site of lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) gene and to explore the correlation between the gene polymorphism of LOX-1 and coronary artery disease（CAD）in the population of Tianjin. Methods: A total of 170 cases confirmed by coronary arteriograghy（CAG） were included in the CAD group and 140 healthy subjects confirmed by CAG were included in the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the polymorphism of 3′-UTR 188 site of LOX-1 gene and to compare its relationship with coronary artery disease between the two groups. Results: There were no significant difference of genotype frequency and allele frequency of 3′-UTR 188 site of LOX-1 between the the CAD group and control group (P>0.05). Conclusion:There is no significant correlation between the polymorphism of 3′-UTR 188 site of LOX-1 and coronary artery disease, therefore the polymorphism can not be confirmed as a predictor of coronary artery disease in the population of Tianjin.

Key words: agglutinins receptors oxidized LDL, coronary artery disease, coronary angiography, genetic alleles, gene frequency, polymorphism