天津医药

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PTEN、p21基因在新疆哈萨克族食管癌中的表达

李卉1,姜孝芳2,王洪江3,庞作良3,古丽努尔·木哈依1,李惠武3   

  1. 1. 新疆医科大学基础医学院
    2. 新疆医科大学
    3. 新疆医科大学附属肿瘤医院
  • 收稿日期:2012-10-22 修回日期:2013-04-22 出版日期:2013-09-15 发布日期:2013-09-15

Expressions of PTEN and p21Genes in Hazak Patients with Esophageal Cancer

LI Hui 1,JIANG Xiao fang1,WANG Hong jiang2,PANG Zuo lian2,MUHAYI Gulinuer 1,LI Hui wu2   

  1. 1. Basic Medicine School of Xinjiang Medical University
    2. Affiliated Tumor Hospital of Xinjiang Medical University
  • Received:2012-10-22 Revised:2013-04-22 Published:2013-09-15 Online:2013-09-15

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摘要：

 【摘要】目的  研究PTEN、p21基因在哈萨克族食管癌中的表达。方法  采用RT-PCR法检测PTEN、p21基因在48例哈萨克族食管癌组织及远端无癌组织中的表达，并分析其表达与肿瘤分化、TNM分期、临床分期、淋巴结转移的关系。结果  PTEN基因在癌组织和远端无癌组织中的表达阳性率分别为75%、45.8%，癌组织高于远端无癌组织（χ2=8.537，P＜0.05）；p21基因在癌组织和远端无癌组织中表达的阳性率分别为95.8%、97.9%，差异无统计学意义（χ2=0.344，P＞0.05）。PTEN和p21表达与食管癌分化程度、不同浸润深度及有无淋巴结转移均无相关性。结论  PTEN、p21基因可能均不是哈萨克族食管癌的主要致病基因。

关键词: 癌, 鳞状细胞, 食管肿瘤, 逆转录聚合酶链反应, 基因, 肿瘤抑制, 原癌基因蛋白质p21（ras）, 基因表达

Abstract:

[Abstract] Objective   To investigate the expressions of PTEN and p21genes in Hazak patients with esophageal can
cer. Methods  The expressions of PTEN and p21genes were detected by RT-PCR in48samples (cancer tissues and normal tissues) of patients with esophageal cancer. The relationship between the expressions of PTEN and p21genes, tumor differentiation, TNM stage, clinical phase and lymph node metastasis were analyzed.  Results  The positive rates of PTEN genewere75% and45.8% in cancer and distant normal tissues. The expression of PTEN was significantly higher in cancer tissues than that of distant normal tissues (χ2=8.537，P＜0.05). The positive rates of p21gene were95.8% and97.9% in cancerand distant normal tissues, and no significant difference between them (χ2=0.344，P＞0.05).There was no correlation between expressions of PTEN and p21and the tumor differentiation, the depth of invasion and lymph node metastasis in esophageal cancer.Conclusion   PTEN and p21genes are not the primary genes for the carcinogenesis of esophageal cancer inHazak.

Key words: carcinoma, squamous cell, Esophageal neoplasms, RT-PCR, gene, tumor suppressor, proto- oncogene proteins p21(ras), 基因表达