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软肝化坚颗粒对肝纤维化模型C57小鼠肝组织Bcl-2及Bax表达的调节作用

叶立红1,王翀奎2,刘莲1,杨莉1,赵召霞1,田岳飏1,戴二黑1   

  1. 1. 石家庄市第五医院
    2. 河北医科大学西山校区
  • 收稿日期:2012-08-02 修回日期:2013-02-20 出版日期:2013-06-15 发布日期:2013-06-15
  • 通讯作者: 叶立红

Regulatory action of Ruangan Huajian granule on hepatic expression of Bcl-2 and Bax in C57 mice with experimental hepatic fibrosis

YE Lihong 1,WANG Chongkui 2,LIU Lian 1,YANG Li 1,ZHAO Zhaoxia 1,TIAN Yueyang 1,DAI Erhei 1   

  1. 1. The Fifth Hospital of Shijiazhuang , Shijiazhuang 050021, China
    2. XiShan Campus of the Hebei Medical University
  • Received:2012-08-02 Revised:2013-02-20 Published:2013-06-15 Online:2013-06-15
  • Contact: YE Lihong

摘要: 摘要 目的    研究软肝化坚颗粒对肝纤维化模型C57小鼠肝组织中凋亡调控相关基因表达的作用。方法  40只C57小鼠随机分为4组,分别为正常对照组、模型对照组、软肝化坚颗粒低剂量组及高剂量组,每组10只。采用四氯化碳腹腔注射诱导形成小鼠肝纤维化模型。采用免疫组织化学染色方法检测肝组织中Bcl-2和Bax的表达,同时进行肝组织纤维化评分(S)。结果   Bcl-2和Bax均主要表达于中央静脉周围凝固性嗜酸性变肝细胞,模型对照组尤为明显,软肝化坚颗粒高、低剂量组凝固性嗜酸性变肝细胞明显减少。模型对照组肝组织肝纤维化评分、Bcl-2和Bax阳性表达量均高显著于正常对照组,(P值分别为0.000;0.000;0.000),而Bcl-2/Bax比值显著低于正常对照组(P=0.003)。软肝化坚颗粒高、低剂量组肝组织Bcl-2阳性表达较模型对照组和正常对照组均明显增强,差异均具有统计学意义(P<0.05), Bax阳性表达量较模型对照组显著减少,但较正常对照组显著增强,差异均具有统计学意义(P<0.05),Bcl-2/Bax比值均显著高于模型对照组和正常对照组(P<0.01,P<0.01)。结论   软肝化坚颗粒可能通过调节肝组织细胞中凋亡相关基因Bcl-2 和Bax的表达,抑制肝细胞凋亡进而发挥抗肝纤维化的作用。

关键词: 细胞凋亡, bcl-2相关X蛋白质, 基因, bcl-2, 肝硬化, 软肝化坚颗粒

Abstract: [Abstract]    Objective   To study the effect of Ruangan Huajian granule on hepatic expression of apoptosis related of gene expression in C57 mice with experimental hepatic fibrosis. Methods    Forty C57 mice were randomly divided into four groups, i.e. normal control group, model control group, model Ruangan Huajian Granule low-dose group and high-dose group. There were 10 mice in every group. C57 mice with experimental hepatic fibrosis were induced by intraperitoneal injection of 10% CCl4. The expression of Bcl-2和Bax in liver tissue of mice was observed by immune histochemical technique. The score of masculine grade and fibrosis stage (S) in liver tissue were also analyzed. Results    Bcl-2和Bax were Mainly express in coagulative liver cells , Model control group particularly obvious. The expression of Bcl-2和Bax and the fibrosis stage in model groups were significantly higher than those in normal control group (P<0.05), the ratio of Bcl-2/Bax Significantly lower than normal control group . the expression of Bcl-2 in Ruangan Huajian Granule low-dose and high-dose group obvious enhancement than those in normal control group and model control group(P<0.05), while the expression of Bax signifecantly reduce(P<0.05), the ratio of Bcl-2/Bax Significantly higher than the normal control group and model control group(P<0.01,P<0.01). Conclusion   Ruangan Huajian granule can modulate the expression of Bcl-2 and Bax in liver tissue ,and inhibit the liver cell apoptisis , Therefor it possesses anti-fibrotic effect.

Key words: cell cycles, bcl-2-associated X protein, genes, bcl-2, Liver cirrhosis, Ruangan Huajian granule