天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (1): 17-19.doi: 10.3969/j.issn.0253-9896.2015.01.005

• 细胞与分子生物学 • 上一篇    下一篇

1,25(OH)2D3对人肾小球系膜细胞增殖及PCNA表达的影响

尹璇 1, 张昊 1, 陈建平 1, 马莉 1, 张春江 2, 杨晓萍 2△#br# #br#   

  1. 1 新疆石河子大学（邮编 832000）；2新疆石河子大学第一附属医院肾病科
  • 收稿日期:2014-08-15 修回日期:2014-10-02 出版日期:2015-01-15 发布日期:2015-01-30
  • 通讯作者: 杨晓萍 E-mail:sbkyxp@163.com
  • 基金资助:
    国家自然科学基金资助项目（81160090）

Effects of 1 ,25(OH)2D3on proliferation and expression of PCNA of human glomerular mesangial cells#br# #br#

YIN Xuan1, ZHANG Hao1, CHEN Jianping1, MA Li1, ZHANG Chunjiang2, YANG Xiaoping2△#br# #br#   

  1. 1 Medical College of Shihezi University, Xinjiang 832000, China; 2 Department of Nephrology, the First Affiliated Hospital of
    Medical College of Shihezi University

  • Received:2014-08-15 Revised:2014-10-02 Published:2015-01-15 Online:2015-01-30

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摘要： 摘要： 目的 探讨 1,25-二羟基维生素 D3 [1,25(OH)2D3]对人肾小球系膜细胞中增殖细胞核抗原（PCNA）表达及细 胞增殖的影响。 方法 体外培养人肾小球系膜细胞，取传代培养至 3~8 代细胞随机分为 4 组： 正常对照组（加含 5%胎 牛血清 DMEM 培养基）， 增殖对照组（EGF 组， 加 10 μg/L 的 EGF）， 一般干预组[VD 组， 加 10- 8 mol/L 1,25(OH)2D3]， 增殖干预组[EGF+VD 组， 加 10 μg/L EGF 及 10-8 mol/L1,25(OH)2D3]， 均作用 48 h。 流式细胞术检测各组细胞周期； Western blot 检测各组 PCNA 表达的情况。 结果 （1）细胞周期。 与正常对照组相比， EGF 组 G1 期细胞明显减少， S、 G2/M 期细胞增多， 增殖指数（PI）较高； VD 组 G1 期细胞明显增多， S、G2/M 期细胞减少， PI 较低； 与 EGF 组相比， VD 组 和 EGF+VD 组 G1 期细胞增多， S、G2/M 期细胞减少， PI 较低， 差异均有统计学意义。（2）系膜细胞中 PCNA 蛋白的表 达。 与正常对照组相比， EGF 组 PCNA 的表达较高， VD 组 PCNA 的表达较低； 与 EGF 组相比， VD 组和 EGF+VD 组 PCNA 的表达较低。 结论 1,25(OH)2D3 通过阻滞细胞周期、 抑制 PCNA 的表达， 从而抑制人肾小球系膜细胞的 增殖。

关键词: 关键词：肾小球系膜细胞, 增殖细胞核抗原, 表皮生长因子, 细胞增殖, 细胞周期, 1,25-二羟基维生素 D3

Abstract: Abstract： Objective To investigate the effects of 1,25-dihydroxyvitamin D3 [1,25 (OH)2D3] on PCNA expression and cell proliferation in human glomerular mesangial cells. Methods The cultured human mesangial cells, which was subcul⁃ tured 3-8 generations, were randomly divided into four groups: normal control group (plus the DMEM medium containing 5% fetal bovine serum), proliferation in the control group (EGF group, plus 10 μg/L of EGF), general intervention group [VD group, plus 10-8 mol/L of 1,25(OH)2D3], proliferation in the intervention group [EGF+VD group, plus 10 μg/L EGF and 10-8 mol/L 1,25(OH)2D3] for treatment of 48 h. The cell cycle was detected by flow cytometry， and the expression of PCNA was detected by Western blot assay in four groups. Results (1) Compared with normal control group, G1 phase cells were signifi⁃ cantly reduced, S, G2/M phase cells were increased and PI index was higher in EGF group. And G1 phase cells were signifi⁃ cantly increased, S and G2/M phase cells were significantly decreased, and PI index was lower in VD group. Compared with the EGF group, G1 phase cells were significantly increased in VD group and EGF+VD group, and S, G2/M phase cells de⁃ creased, PI index was lower. (2) Compared with normal control group, the expression of PCNA was higher in EGF group, and lower in VD group. Compared with EGF group, the expression of PCNA was lower in VD group and EGF+VD group. Conclu⁃ sion 1,25 (OH) 2D3 inhibits the proliferation of human glomerular mesangial cells by arresting cell cycle and inhibiting the expression of PCNA protein.

Key words: Key words： glornerular mesangial cells, proliferating cell nuclear antigen, epidermal growth factor, cell proliferation;
cell cycle, 1,25 (OH)2D3