天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (4): 364-366.doi: 10.11958/j.issn.0253-9896.2015.04.008

• 实验研究 • 上一篇    下一篇

三七三醇皂苷对糖尿病大鼠学习记忆能力及星形胶质细胞活性的影响

李春桃1，韩言秀2，杜晓薇2，左中夫1△   

  1. 1辽宁医学院解剖学教研室（邮编121001）；2辽宁医学院基础医学院
  • 收稿日期:2014-03-27 修回日期:2014-11-12 出版日期:2015-04-15 发布日期:2015-04-13
  • 通讯作者: 左中夫 E-mail:zuozhongfu9807@163.com
  • 基金资助:
    国家自然科学基金资助项目（81300931）；辽宁省自然科学基金资助项目（2013022055）；辽宁省大学生创新创业训练计划项目（201310160004）；辽宁医学院“校长基金”资助项目（XZJJ20130105-03）；2013年度辽宁医学院大学生科技创新项目

Roles of panaxtrial saponins in cognition and memory of diabetic rat and in activity of astrocytes

LI Chuntao1, HAN Yanxiu2, DU Xiaowei2, ZUO Zhongfu1△   

  1. 1 Department of Human Anatomy, Liaoning Medical University, Jinzhou 121001, China; 2 College of Basic Medical Science, Liaoning Medical University
  • Received:2014-03-27 Revised:2014-11-12 Published:2015-04-15 Online:2015-04-13
  • Contact: ZUO Zhongfu E-mail:zuozhongfu9807@163.com

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摘要： 摘要：目的探讨三七三醇皂苷（PTS）对糖尿病大鼠学习记忆的改善作用，并从星形胶质细胞角度揭示其机制。方法24 只SD 大鼠随机分为对照组、糖尿病组及PTS 治疗组，每组8 只。于SD 大鼠腹腔注射链脲佐菌素诱导糖尿病模型，对照组为正常SD 大鼠，PTS 治疗组为糖尿病大鼠给予PTS 治疗。检测大鼠血糖和体质量，3 个月后，水迷宫实验观察各组大鼠的学习记忆功能，免疫组化染色检测脑海马区星形胶质细胞形态，Western blot 法检测神经胶质纤维酸性蛋白（GFAP）及胶质细胞源性神经营养因子（GDNF）表达。结果糖尿病组较对照组学习记忆能力减退，脑海马区星形胶质细胞胞体萎缩、突起变细，脑海马区GFAP 及GDNF 表达明显减少（均P < 0.05）。PTS 组较糖尿病组学习记忆能力改善，脑海马区星形胶质细胞形态改善、GFAP 及GDNF 表达明显恢复（均P < 0.05）。结论PTS 能恢复糖尿病大鼠脑海马区星形胶质细胞活性，恢复GDNF 表达，提高糖尿病大鼠学习记忆功能。

关键词: 糖尿病, 神经胶质原纤维酸性蛋白质, 模型, 动物, 学习记忆, 三七三醇皂苷, 星形胶质细胞, 胶质细胞源性神经营养因子

Abstract: Abstract：Objective To explore protective effects of panaxtrial saponins (PTS) on cognition and memory of diabetic rats and to reveal its mechanism by which might involve regulating activity of astrocytes. Methods SD rats (n=24) were ran⁃ domly assigned into control, diabetic and PTS-treated groups (n=8 in each group). Rat diabetic model was induced through streptozotocin injection intraperitoneally. Rats in control group were native rats, and rats in PTS-treated group were diabetic rats that were administered with PTS. Body weight and blood glucose were monitored through the experiments. Three months later, state of cognition was examined by methods of water maze. Hippocampal astrocyte morphology were detected by immu⁃ nohistochemistry, and the expression of glial cell line-derived neurotrophic factor (GDNF) and glial fibrillary acidic protein (GFAP) in hippocampus were revealed by Western blot. Results Compared with control group, diabetic group showed cog⁃ nitive dysfunction, atrophic astrocyte soma, shrinked astrocyte processes, and down-regulation of hippocampal GFAP and GDNF (P < 0.05). Compared with diabetic group, PTS-treated group exhibited improved cognition and morphology of hippo⁃ campal astrocyte, and reversed expression of GFAP and GDNF in diabetic hippocampus (P < 0.05). Conclusion PTS re⁃ versed astrocytic reactivity as well as expression of GDNF and GFAP in diabetic hippocampus and ameliorated diabetic cog⁃ nitive dysfunction.

Key words: diabetes mellitus, glial fibrillary acidic protein；models, animal；learning and memory；panaxtrial saponins, astrocytes；GDNF