天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (1): 50-52.doi: 10.11958/58997

• 细胞与分子生物学 • 上一篇    下一篇

全反式维甲酸对高糖诱导肾小管上皮细胞增殖及凋亡的影响

陈艳霞， 房向东， 黄翀， 秦晓华， 邹宏昌， 徐承云， 徐高四， 涂卫平△   

  1. 南昌大学第二附属医院肾内科 （邮编330006）
  • 收稿日期:2015-05-27 修回日期:2015-08-11 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: △通讯作者 E-mail： 891289589@qq.com E-mail:tuweiping6102@sina.com
  • 作者简介:陈艳霞 （1988）， 女， 硕士， 主要从事延缓慢性肾脏病进展的研究
  • 基金资助:

     江西省卫生厅科技项目 （20151BBG70173）

Effects of ATRA on high glucose-induced proliferation and apoptosis of renal tubular epithelial cells

CHEN Yanxia, FANG Xiangdong, HUANG Chong, QIN Xiaohua, ZOU Hongchang, XU Chengyun, XU Gaosi, TU Weiping△   

  1. Department of Nephrology, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
  • Received:2015-05-27 Revised:2015-08-11 Published:2016-01-15 Online:2016-01-15
  • Contact: △Corresponding Author E-mail： 891289589@qq.com E-mail:tuweiping6102@sina.com

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摘要： 摘要： 目的 探讨全反式维甲酸 （ATRA） 对高糖诱导人 HK-2 细胞增殖及凋亡的影响， 以期延缓糖尿病肾病（DN）。方法 体外培养 HK-2 细胞， 随机分为 6 组： 空白组 （未加任何刺激物）、 高糖组 （加 D-葡萄糖 30 mmol/L）、 高渗组 （加入甘露醇 24.5 mmol/L）、 低浓度 ATRA 组 （加入 ATRA 1×10-7 mol/L+D-葡萄糖 30 mmol/L）、 中浓度 ATRA 组（加入 ATRA 1×10-6 mol/L+D-葡萄糖 30 mmol/L）、 高浓度 ATRA 组 （加入 ATRA 1×10-5 mol/L+D-葡萄糖 30 mmol/L）。各组细胞培养 48 h。MTT 法检测各组细胞增殖情况， 流式细胞术检测各组细胞凋亡情况。结果 空白组与高渗组细胞光密度 （OD） 值和凋亡率差异无统计学意义。高糖组较空白组、 高渗组细胞增殖减少， 凋亡率增加； 低浓度、 中浓度、 高浓度 ATRA 组细胞增殖均较高糖组增加， 且低、 中及高浓度 ATRA 组依次增加， 凋亡率较高糖组减少， 且低、 中及高浓度 ATRA 组依次减少 （均 P＜0.05）。结论 ATRA 可促进高糖诱导的 HK-2 细胞增殖， 抑制其凋亡， 并与 ATRA 浓度可能具有一定的依赖性。

关键词: 细胞增殖, 细胞凋亡, 体外研究, 全反式维甲酸, 肾小管上皮细胞

Abstract: Abstract:Objective To investigate the effect of all-trans retinoic acid (ATRA) on high glucose-induced proliferation and apoptosis in human HK-2 cells， and to provide a reference for slowing down the progress of diabetic nephropathy (DN). Methods HK-2 cells were cultured in vitro and were divided into six groups: blank group (without stimulants), high glu⁃ cose group (D-glucose 30 mmol/L), high permeability group (mannitol 24.5 mmol/L) and low concentration of ATRA group (ATRA 30 mmol/L 1×10- 7 mol/L+D-glucose 30 mmol/L), middle concentration of ATRA group (ATRA 30 mmol/L 1×10- 6 mol/L +D-glucose 30mmol/L) and high concentration of ATRA group (ATRA 1×10-5 mol/L+D-glucose 30 mmol/L). Cells of six groups were cultured for 48 hours. MTT assay was used for detection of cell proliferation. Flow cytometry was used for de⁃ tection of cell apoptosis. Results There were no significant differences in OD value and apoptotic rate between the blank group and the high permeability group. The cell proliferation was decreased in high glucose group than that of blank group and high permeability group， but the apoptotic rate was increased. The cell proliferation was significantly higher in low con⁃ centration, medium concentration and high concentration of ATRA groups than that of high glucose group, and the apoptotic rate was lower than that of high glucose group, and the changes were in a concentration dependent manner (P＜0.05). Con⁃ clusion ATRA can promote the proliferation of HK-2 cells induced by high glucose, and inhibit the apoptosis, and which has a certain relevance with the concentration of ATRA.

Key words: cell proliferation, cell apoptosis, in vitro, all-trans retinoic acid, renal tubular epithelial cells