天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (10): 1263-1267.doi: 10.11958/20160128

• 临床研究 • 上一篇    下一篇

急性 STEMI 患者血同型半胱氨酸与 Kv1.3 通道、肌钙蛋白关系的研究

王玉岭 ，冯建宇，曾祥飞，杨绍兵，严宁，贾绍斌   

  1. 1 延安， 洛川县妇幼保健院（邮编 727400）； 2 宁夏医科大学； 3 第四军医大学西京医院心脏外科； 4 宁夏医科大学总医院心脏中心
  • 收稿日期:2016-03-08 修回日期:2016-07-19 出版日期:2016-10-15 发布日期:2016-10-21
  • 通讯作者: 贾绍斌 E-mail:2201823957@qq.com
  • 作者简介:王玉岭（1981）， 女， 硕士在读， 主要从事动脉粥样硬化机制研究
  • 基金资助:

    宁夏医科大学优势学科群建设科研项目（xy2014016）

Study on relationship between plasma homocysteine, Kv1 .3 channel and troponin in patients with acute ST-segment elevation myocardial infarction

WANG Yuling, FENG Jianyu, ZENG Xiangfei, YANG Shaobing, YAN Ning, JIA Shaobin   

  1. 1 Maternal and Child Health Hospital of Luochuan County, Yanan 727400， China；2 Ningxia Medical University； 3 Department of Cardiovascular Surgery, Xijing Hospital, the Fourth Military Medical University; 4 Department of Cardiology, General Hospital of Ningxia Medical University
  • Received:2016-03-08 Revised:2016-07-19 Published:2016-10-15 Online:2016-10-21
  • Contact: JIA Shaobin E-mail:2201823957@qq.com

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摘要： 目的 探讨急性 ST 段抬高型心肌梗死（STEMI）患者血浆同型半胱氨酸（Hcy）水平与淋巴细胞电压门控性钾通道（Kv1.3 通道）、Kv1.3 通道与心肌肌钙蛋白 I（cTnI）水平的相关性。 方法 80 例 STEMI 患者根据血浆 Hcy 水平分为 STEMI 合并高同型半胱氨酸血症（Hhcy）组（STEMI+Hhcy 组， Hcy >15 μmol/L， n=41）和对照组（STEMI 组， Hcy≤15 μmol/L， n=39）。 抽取血标本后全自动生化仪测定 Hcy、cTnI 及血脂等指标， 密度梯度离心法提取淋巴细胞，实时荧光定量 PCR 检测 Kv1.3 mRNA 表达， Western blot 检测 Kv1.3 蛋白表达。 结果 STEMI+Hhcy 组 cTnI 水平高于 STEMI 组（μg/L： 22.997±5.880 vs. 12.881±6.343； P＜ 0.01）。 多元线性回归分析显示年龄、性别、高血压病、糖尿病、吸烟史、家族史、三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）对 Hcy 无明显影响（P＞ 0.05）。 STEMI+Hhcy 组 Kv1.3 通道 mRNA 和蛋白相对表达量（1.35±0.14 和 0.85±0.12） 较 STEMI 组升高（1.00±0.07 和 0.64±0.05， P＜ 0.05）。 Hcy 水平与 Kv1.3 通道 mRNA 和蛋白表达水平呈正相关（r 分别为 0.299、0.542， P＜ 0.05）； Kv1.3 通道蛋白表达水平与 cTnI 水平呈正相关（r=0.644， P＜ 0.05）。 结论 Hcy 可能对淋巴细胞 Kv1.3 通道表达起着一定的作用， 导致血浆 cTnI 水平升高。

关键词: 心肌梗死, 高同种半胱氨酸血症, Kv1.3 钾通道, 淋巴细胞, 肌钙蛋白 I, 急性 ST 段抬高型心肌梗死

Abstract: Objective To investigate the relationship between plasma homocysteine (Hcy), Kv1.3 channel and cardiac troponinI (cTnI) in patients with acute ST-segment elevation myocardial infarction (STEMI). Methods According to the level of Hcy, 80 STEMI patients were divided into STEMI with Hhcy group (Hcy > 15 μmol/L, n=41) and control group (STEMI group, Hcy≤15 μmol/L, n=39). The Hcy, blood lipid and cTnI were detected with automatic biochemistry analyzer, respectively. Peripheral lymphocytes were isolated by ficoll density gradient centrifugation. Real-time PCR was used to detect mRNA expression of Kv1.3, and Western blot assay was used to detect protein expression of Kv1.3. Results cTnI concentrations were obviously higher in STEMI with Hhcy group than those in STEMI group（μg/L： 22.997 ± 5.880 vs. 12.881 ± 6.343； P＜ 0.01）. Multiple linear regression analysis showed that age, gender, hypertension, diabetes mellitus, smoking, family history, total cholesterol (TC), triglyceride (TG), high density lipoprotein- cholesterol (HDL- C) and low density lipoprotein-cholesterol (LDL-C) had no obvious influence on Hcy (P＞ 0.05). The relative expression levels of Kv1.3 mRNA and protein were significantly higher in STEMI with Hhcy group (1.35±0.14, 0.85±0.12) than those in STEMI group (1.00± 0.07, 0.64± 0.05, P＜ 0.05). Moreover, there was a positive relation between Hcy level and the mRNA and protein expression of Kv1.3 channel (r=0.299, r=0.542, P＜ 0.05). There was a positive relation between protein expression levels of Kv1.3 channel and cTnI (r=0.644, P＜ 0.05). Conclusion Our results support that Hcy could exacerbate the concentration of cTnI through playing an important role in the Kv1.3 mRNA and protein expression in lymphocytes.

Key words: myocardial infarction, hyperhomocysteinemia, Kv1.3 potassium channel, lymphocytes, troponin I, acute ST-segment elevation myocardial infarction