天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (2): 164-167.doi: 10.11958/20160973

• 实验研究 • 上一篇    下一篇

青蒿琥酯可抑制高糖诱导的肾小球系膜细胞增殖模型中 NF-κB、 MCP-1 的表达

黄雪娟， 苏珂△， 龙艳， 荀靖琼， 王海旭   

  1. 桂林医学院附属医院内分泌科 （邮编 541004）
  • 收稿日期:2016-09-07 修回日期:2016-12-05 出版日期:2017-02-15 发布日期:2017-02-14
  • 通讯作者: △通讯作者 E-mail：13978316336@163.com E-mail:972592082@qq.com
  • 作者简介:黄雪娟 （1992）， 女， 硕士在读， 主要从事糖尿病及其并发症研究
  • 基金资助:
    桂林市科技局 2014 年科技攻关课题 （20140310-2-6）

Artesunate supresses NF-κB and MCP-1 expressions in high glucose-induced rat mesangial cell proliferation model

HUANG Xue-juan, SU Ke△, LONG Yan, XUN Jing-qiong, WANG Hai-xu   

  1. Department of Endocrinology, Affiliated Hospital of Guilin Medical College, Guangxi 541004, China
  • Received:2016-09-07 Revised:2016-12-05 Published:2017-02-15 Online:2017-02-14
  • Contact: △Corresponding Author E-mail: 13978316336@163.com E-mail:972592082@qq.com

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摘要： 目的 通过观察青蒿琥酯 （Art） 对高糖培养条件下大鼠肾小球系膜细胞 （HBZY-1） 的增殖、 凋亡及核因子（NF） -κB、 单核细胞趋化蛋白-1（MCP-1）表达的变化， 探讨 Art 对糖尿病肾病保护性作用的机制。方法 传代培养大鼠肾小球系膜细胞 HBZY-1， 分为低糖 （5.6 mmol/L 葡萄糖） 组、 高糖 （25 mmol/L 葡萄糖） 组、 高糖+不同浓度青蒿琥酯（10、 20、 30 mg/L）组。48 h 后采用四甲基偶氮唑盐微量比色（MTT）法观察细胞增殖能力改变； 流式细胞仪 Annexin V-FITC/PI 双染法检测细胞凋亡指数； 酶联免疫吸附试验（ELISA）法检测细胞上清液 NF-κB、 MCP-1 的含量。结果 （1）经高糖处理 48 h 后， HBZY-1 细胞较低糖组增殖能力及凋亡率均增加， 细胞上清液 NF-κB、 MCP-1 浓度升高。（2）经 Art 干预后， 与高糖组比较， HBZY-1 细胞增殖抑制， 细胞凋亡率增加， 细胞上清液 NF-κB、 MCP-1 浓度降低， 其干预作用呈现剂量依赖性 （P＜0.05）。结论 Art 能剂量依赖性地逆转高糖的作用。Art 对高糖诱导下肾小球系膜细胞的增殖与凋亡以及炎症因子的作用机制可能为临床上糖尿病肾病提供新的治疗策略。

关键词: 糖尿病肾病, 细胞凋亡, 肾小球系膜细胞, NF-κB, 单核细胞趋化蛋白-1, 青蒿琥酯

Abstract: Objective To investigate the effects of artesunate (Art) on cell proliferation, apoptosis, nuclear factor (NF)- κB and monocyte chemotactic protein 1 (MCP-1) expressions induced by high glucose in rat renal mesangial cells (HBZY- 1), and the mechanism thereof. Methods HBZY-1 cells were cultured and divided into normal glucose group (5.6 mmol/L), high glucose group (25 mmol/L) and high glucose with different concentrations of Art (10 mg/L, 20 mg/L, 30 mg/L) groups. MTT assay was used to detect the cell proliferation after 48 h. The apoptotic rate was evaluated by flow cytometry with Annexin V-FITC/PI double stains. The protein levels of NF-κB and MCP-1 in the cell culture supernatant were determined using ELISA. Results High glucose induced apoptosis and proliferation in HBZY-1 cells, and the expressions of NF-κB and MCP- 1 in the supernatant were also increased (P＜0.05). After treatment with Art, the proliferation was obviously abolished, and the apoptosis was increased, and the expressions of NF-κB and MCP-1 in the supernatant were decreased in HBZY- 1 cells. The effects of Art showed a dose- dependent manner (P＜0.05). Conclusion Artesunate treatment can reverse the effect of high glucose in HBZY- 1 cells in a dose- dependent manner, which may provide a new therapeutic strategy for diabetic nephropathy.

Key words: diabetic nephropathies, apoptosis, mesangial cells, NF- kappa B, monocyte chemotactic protein 1, Artesunate