天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (4): 371-375.doi: 10.11958/20171106

• 实验研究 • 上一篇    下一篇

黄芪甲苷对异丙肾上腺素诱导大鼠 心肌肥厚的保护作用研究

段立鸣，孙玉红，王方△   

  1. 作者单位：郑州，中国人民解放军第153中心医院（邮编450000） 作者简介：段立鸣（1975），女，硕士研究生，副主任药师，主要从事临床药学方面研究 △通讯作者 E-mail: 153adr@163.com
  • 收稿日期:2017-10-17 修回日期:2018-01-26 出版日期:2018-04-15 发布日期:2018-04-15
  • 通讯作者: 段立鸣 E-mail:153adr@163.com

Protective effects of astragaloside on isoproterenol induced myocardial hypertrophy in rats

DUAN Li-ming, SUN Yu-hong, WANG Fang△   

  1. Chinese People’s Liberation Army 153 Central Hospital, Zhengzhou 450000, China △Correspongding Author E-mail: 153adr@163.com
  • Received:2017-10-17 Revised:2018-01-26 Published:2018-04-15 Online:2018-04-15

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摘要： 摘要：目的 探讨黄芪甲苷对异丙肾上腺素（ISO）诱导的大鼠心肌肥厚的保护作用机制。方法 健康SPF级SD 大鼠24只，随机分为4组，对照组（与ISO组等体积的生理盐水腹腔注射，之后与黄芪甲苷组等体积生理盐水灌胃）、 ISO组［ISO 3 mg/ （kg·d）腹腔注射，与黄芪甲苷等体积生理盐水灌胃］、黄芪甲苷组［与ISO等体积生理盐水腹腔注射， 黄芪甲苷溶液5 mg/ （kg·d）灌胃］、黄芪甲苷+ISO组［ISO 3 mg/ （kg·d）腹腔注射，黄芪甲苷溶液5 mg/ （kg·d）灌胃］，每 组6只，共处理14 d。处理结束后取大鼠左心室，计算左心室质量指数（左心室质量/体质量）。HE染色观察左心室心 肌肥厚情况，比较心肌横截面积的差异。以二氯荧光素（DCF）为探针检测心肌线粒体活性氧（ROS）生成速率； Western blot 法检测大鼠心肌NADPH氧化酶4（NOX4）和心房利钠肽（ANP）蛋白表达水平；RT-qPCR检测大鼠心肌 ANP在转录水平的表达情况。结果 与对照组相比，ISO组左心室质量指数明显升高，心肌横截面积扩大，心肌线粒 体ROS生成速率加快，NOX4蛋白、ANP mRNA及蛋白表达水平均明显升高（P < 0.05）。而ISO+黄芪甲苷组与ISO组 相比，上述指标均明显改善，主要表现为心肌横截面积缩小，ROS生成速率减慢，NOX4蛋白、ANP mRNA及蛋白表达 明显下降，差异均有统计学意义（P＜0.05）。结论 黄芪甲苷对ISO诱导的大鼠心肌肥厚具有确切的保护作用，且该 保护作用可能与黄芪甲苷降低氧化应激反应相关。

关键词: 黄芪甙, 异丙肾上腺素, 大鼠, Sprague-Dawley, 心肌病, 肥厚性, 氧化性应激

Abstract: Abstract：Objective To investigate the protective mechanism of astragaloside on isoproterenol (ISO) induced myocardial hypertrophy in rats. Methods Twenty-four healthy SPF rats were randomly divided into 4 groups: astragaloside+ISO group [3 mg/(kg·d) ISO intraperitoneal injection, astragaloside 5 mg/(kg·d) gastric lavage], control group (equal volume of normal saline intraperitoneal injection, followed by equal volume of normal saline gastric lavage), ISO group [3 mg/(kg·d) ISO intraperitoneal injection, and normal saline gastric lavage] and astragaloside group [equal volume of normal saline intraperitoneal injection, astragaloside 5 mg/(kg·d) gastric lavage]. Six rats were included in each group and treated for 14 days. After treatment, the left ventricle of rats was taken, and the left heart index (left heart weight/ body weight) was calculated after weighing. The left ventricular tissue was isolated and the occurrence of myocardial hypertrophy was observed by HE staining to compare the myocardial cross-sectional area. Two chlorine fluorescence (DCF) probes to detect the generation rate of myocardial mitochondrial reactive oxygen species (ROS). The protein expression levels of rat myocardial NADPH oxidase 4 (NOX4) and atrial natriuretic peptide (ANP) were detected by Western blot assay. The mRNA expression of myocardial ANP was detected by real-time qPCR. Results Compared with the control group, the left heart index was significantly increased, the myocardial cross-sectional area enlarged, the ROS production rate of myocardial mitochondria increased, NOX4 protein, ANP mRNA and protein expression levels were significantly increased in ISO group (P＜0.05). Compared with the ISO group, the above indicators were significantly improved in ISO + astragaloside group, and the major performance included the reduced myocardial cross-sectional area, decreased ROS generation rate, significantly decreased NOX4 protein, ANP mRNA and protein expressions (P＜0.05). Conclusion Astragaloside has an exact protective effect on myocardial hypertrophy induced by ISO in rats, and which may be related with the decreased oxidative stress by astragaloside.

Key words: ASTRAGALIN, isoproterenol, rats, Sprague-Dawley, cardiomyopathy, hypertrophic, oxidative stress