天津医药

天津医药 ›› 2018, Vol. 46 ›› Issue (4): 368-371.doi: 10.11958/20180122

• 实验研究 • 上一篇    下一篇

内质网应激对小鼠卵巢卵泡发育的影响

黄宁，于洋△   

  1. 基金项目：国家自然科学基金资助项目（81771580） 作者单位：北京大学第三医院妇产科生殖医学中心，生殖内分泌与辅助生殖技术北京市重点实验室，教育部辅助生殖重点实验室（邮编 100191） 作者简介：黄宁（1990），女，硕士在读，主要从事生殖生物学与辅助生殖技术方面研究 △通讯作者 E-mail: yuyang5012@hotmail.com
  • 收稿日期:2018-01-19 修回日期:2018-02-27 出版日期:2018-04-15 发布日期:2018-04-15
  • 通讯作者: 于洋 E-mail:yuyang5012@hotmail.com
  • 基金资助:
    国家自然科学基金面上项目

The effect of endoplasmic reticulum stress on ovarian follicular development in mice

HUANG Ning, YU Yang△   

  1. Center of Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, China △Corresponding Author E-mail: yuyang5012@hotmail.com
  • Received:2018-01-19 Revised:2018-02-27 Published:2018-04-15 Online:2018-04-15
  • Contact: Yang yuYu E-mail:yuyang5012@hotmail.com

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摘要： 摘要：目的 探索小鼠卵巢内质网应激对卵泡发育的影响。方法 对照组小鼠4只，实验组小鼠6只，应用经典 内质网应激诱导物衣霉素经腹腔注射诱导小鼠卵巢内质网应激激活，实时荧光定量逆转录聚合酶链反应（RT qPCR）检测未折叠蛋白质应答（UPR）标志分子HSPA5、CHOP、ATF4 mRNA表达情况，明确小鼠卵巢内质网应激激活 状态，苏木精伊红染色（HE染色）检测小鼠卵巢卵泡发育状态，明确内质网应激对小鼠卵巢卵泡发育的影响。结果 衣霉素的处理明显上调了小鼠卵巢内质网应激标志分子的表达，与对照组相比，衣霉素处理组小鼠卵巢卵泡发育明 显受限。结论 内质网应激的激活明显抑制了小鼠卵巢卵泡的发育

关键词: 内质网, 应激, 卵泡, 小鼠, 近交C57BL, 未折叠蛋白质应答

Abstract: Abstract: Objective To explore the effect of endoplasmic reticulum (ER) stress on ovarian follicular development in mice. Methods The model mice were divided into control group (n=4) and tunicamycin group (n=6). The endoplasmic reticulum stress activation was induced in mice by intraperitoneal injection of tunicamycin, which was the classical endoplasmic reticulum stress inducer. The marker gene HSPA5, CHOP and ATF4 related to unfolded protein response (UPR) were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), and which was to identify whether ER stress was activated. H&E staining was used to determine the effect of endoplasmic reticulum stress on ovarian follicular development in mice. Results Compared with the control group, tunicamycin treatment significantly increased the expression of UPR related genes and significantly reduced the number of ovarian follicles. Conclusion The activation of endoplasmic reticulum stress significantly inhibits the mouse ovarian follicular development.

Key words: endoplasmic reticulum, stress, ovarian follicle, mice, inbred C57BL, unfolded protein response