天津医药

天津医药 ›› 2019, Vol. 47 ›› Issue (2): 113-118.doi: 10.11958/20181265

• 细胞与分子生物学 •    下一篇

新化合物OSU-03012对结肠癌细胞增殖、迁移以及凋亡的影响

李玉琴， 施建平△， 金怒云， 步丽梅， 王凯， 田培营   

  1. 上海市浦东医院 （复旦大学附属浦东医院） 消化内科 （邮编201399）
  • 收稿日期:2018-08-22 修回日期:2018-12-06 出版日期:2019-02-15 发布日期:2019-02-15
  • 通讯作者: 李玉琴 E-mail:liyu_qii@sina.com
  • 基金资助:
    上海市浦东新区科技发展基金民生科研项目

Effects of new compound OSU-03012 on proliferation, migration and apoptosis of colon cancer cells

LI Yu-qin, SHI Jian-ping△, JIN Nu-yun, BU Li-mei， WANG Kai, TIAN Pei-ying   

  1. Department of Digestion, Shanghai Pudong Hospital (Fudan University Pudong Medical Center), Shanghai 201399, China
  • Received:2018-08-22 Revised:2018-12-06 Published:2019-02-15 Online:2019-02-15

PDF (PC)

3861

摘要： 摘要： 目的 在细胞水平上初步探讨新化合物OSU-03012抗结肠癌的机制。方法 分别用1、 3、 5和10 μmol/L OSU-03012化合物干预的人结肠癌细胞株HCT-116作为研究对象， 以等体积二甲基亚砜 （DMSO） 处理的HCT-116 细胞作为对照组。CCK-8法检测各组细胞干预24、 48、 72 h时的抑制率， 划痕实验和Transwell实验检测各组细胞迁移能力， 流式细胞术检测各组细胞凋亡情况， 免疫荧光、 Western blot检测各组细胞Bcl-2、 Caspase-3蛋白表达情况， Real-time PCR检测各组细胞Bcl-2、 Caspase-3 mRNA表达情况。结果 对照组、 1 μmol/L组、 3 μmol/L组、 5 μmol/L 组和10 μmol/L组的细胞抑制率、 凋亡率呈逐渐升高趋势， 而细胞迁移距离、 细胞迁移数目呈逐渐减小或降低趋势， 组间多重比较差异均有统计意义 （均P＜0.05）； 3、 5、 10 μmol/L组Bcl-2 mRNA和蛋白表达量低于对照组， 而Caspase-3 mRNA和蛋白表达量高于对照组 （均P＜0.05）。结论 新化合物OSU-03012可抑制结肠癌细胞增殖与迁移， 并促进结肠癌细胞凋亡， 且呈剂量依赖效应。

关键词: 结肠肿瘤, 细胞增殖, 细胞运动, 细胞凋亡, OSU-03012, 环氧合酶-2

Abstract: Abstract: Objective To preliminarily study the anti-colon mechanism of the new compound OSU-03012 at cellular level. Methods The human colon cancer cell line HCT-116, which was intervened with 1 μmol /L, 3 μmol /L, 5 μmol /L and 10 μmol /L of OSU-03012, was used as subjects. HCT-116 cells treated with the same volume of dimethyl sulfoxide (DMSO) were taken as the control group. The inhibition rates of cells in each group after 24, 48 and 72 hours of intervention were detected by CCK-8 method, and the migration ability of cells was detected by scratching test and Transwell assay. The apoptosis of cells was detected by flow cytometry assay, and the expressions of Bcl-2 and Caspase-3 proteins were detected by immunofluorescence assay and Western blot assay. The expressions of Bcl-2 and Caspase-3 mRNA were detected by Real-time PCR. Results The cell inhibition rate and apoptosis rate gradually increased while the cell migration distance and cell migration number gradually decreased in the control group, 1 μmol/L group, 3 μmol/L group, 5 μmol/L group and 10 μmol / L group. There were statistical significance in the multiple comparison between the groups (all P＜0.05). The mRNA and protein expression of Bcl-2 were lower in 3 μmol/L group, 5 μmol/L group and 10 μmol/L group than those in the control group, while mRNA and protein expression of Caspase-3 were higher than those in the control group (both P＜ 0.05). Conclusion The new compound OSU-03012 can inhibit the proliferation and migration of colon cancer cells and promote the apoptosis of colon cancer cells in a dose-dependent manner.

Key words: colonic neoplasms, cell proliferation, cell movement, apoptosis, OSU-03012, cyclooxygenase-22