天津医药

天津医药 ›› 2020, Vol. 48 ›› Issue (8): 731-735.doi: 10.11958/20193687

• 临床研究 • 上一篇    下一篇

TRPC3基因多态性与原发性高血压患者左心室肥厚的关系#br#

蒋永超1，孙晓靖2，陈玉岚1△，张俊仕1，珠勒皮亚司马义1，徐新娟1，张向阳1
  

  1. 1新疆医科大学第一附属医院高血压科（邮编830054）；2新疆医科大学第二附属医院重症医学科
  • 收稿日期:2019-12-06 修回日期:2020-06-04 出版日期:2020-08-15 发布日期:2020-08-12
  • 通讯作者: 陈玉岚 E-mail:sheliachen@sina.com
  • 作者简介:蒋永超（1994），男，硕士在读，主要从事高血压及相关疾病的临床与基础研究
  • 基金资助:
    新疆维吾尔自治区自然科学基金项目（2016D01C211）

Correlation between gene polymorphism of TRPC3 and left ventricular hypertrophy inpatients with essential hypertensive #br#

JIANG Yong-chao1, SUN Xiao-jing2, CHEN Yu-lan1△, ZHANG Jun-shi 1, ZHU LEPIASimayi 1, XU Xin-juan1, ZHANG Xiang-yang1#br#   

  • Received:2019-12-06 Revised:2020-06-04 Published:2020-08-15 Online:2020-08-12
  • Contact: CHEN Yu-lan E-mail:sheliachen@sina.com

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摘要： 目的　探讨TRPC3基因多态性与原发性高血压患者左心室肥厚（LVH）的关系。方法　305例原发性高血压患者根据左心室质量指数（LVMI）分为单纯高血压224例（对照组）和高血压合并LVH 81例（LVH组）。分析2组年龄、吸烟史等一般资料差异。留取外周血标本，采用Sequenom MassARRAY® SNP检测技术对TRPC3基因单核苷酸多态性（SNP）位点Rs2292232、Rs4995894、Rs4292355进行基因分型。Logistic回归分析影响患者LVH的因素。结果　（1）2组年龄、吸烟史、饮酒史、家族史、尿素、肌酐、空腹血糖、三酰甘油、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、24 h平均舒张压（24 h DBP）差异无统计学意义（P＞0.05），LVH组较对照组男性比例降低，而24 h平均收缩压（24 h SBP）、体质量指数（BMI）升高（P＜0.05）。（2）2组Rs4995894及Rs4292355位点等位基因频率、基因型频率分布差异无统计学意义（P＞0.05），TRPC3基因SNP位点Rs2292232基因型频率和等位基因频率分布差异有统计学意义（P＜0.05），其中LVH组Rs2292232位点TT基因型频率较对照组更高（P＜0.05）。（3）Logistic回归分析显示，女性（OR=0.332，95%CI：0.181～0.610）、较高24 h SBP（OR=1.035，95%CI：1.014～1.056）、TRPC3基因SNP Rs2292232 TT基因型（OR=2.105，95%CI：1.109～3.995）为原发性高血压患者发生LVH的独立危险因素（均P＜0.05）。结论　TRPC3基因Rs2292232位点SNP与高血压患者发生LVH有关，其中TT基因型患者更易发生LVH。

关键词: 瞬时受体电位通道, 多态性, 单核苷酸, 基因频率, 原发性高血压, 左心室肥厚, TRPC3

Abstract: Objective　To study the relationship between gene polymorphism of TRPC3 and left ventricular hypertrophy(LVH) inpatients with essential hypertensive (EH). Methods　Genomic DNA was isolated from blood of 305 EH patients, including 205 male patients and 100 female patients. According to the left ventricular mass index (LVMI)，the patients were divided into simple hypertension group (normal group, n=224) and EH+LVH group (n=81). The differences in general data such as age and smoking history were analyzed between the two groups.The single nucleotide polymorphism (SNP) sites Rs2292232, Rs4995894 and Rs4292355 of TRPC3 gene were genotyped by Sequenom MassARRAY® SNP detection technology. Logistic regression analysis was used for analyzing factors affecting LVH inpatients. Results　(1) There were no significant differences in age, smoking history, drinking history, family history, urea, creatinine, fasting blood glucose, triglycerides, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and 24-hour mean diastolic blood pressure (24 h DBP) between the two groups. The male proportion was lower in EH+LVH group than those of normal group. Values of 24 h DBP and body massindex (BMI) were higher in EH+LVH group than those of normal group (P＜0.05). (2) There were no significant differences in gene frequency and genotype frequency distribution of Rs4995894 and Rs4292355 between the 2 groups (P＞0.05), and there were significant differences in the frequency distribution of the TRPC3 gene SNP site Rs2292232 (P＜0.05). The distribution frequency of Rs2292232 point TT genotype was significantly higher in the EH + LVH group than that of normal control group (P＜0.05). (3) The Logistic regression analysis showed that woman (OR=0.332,95%CI:0.181-0.610，P＜0.05), higher 24-hour DBP (OR=1.035,95%CI:1.014-1.056,P＜0.05) and the TRPC3 gene SNP Rs2292232 TT genotype (OR=2.105，95%CI:1.109-3.995，P＜0.05) were independent risk factors for LVH in hypertensive patients. Conclusion　The Rs2292232 SNP of TRPC3 gene is risk factor for EH patients with LVH, and TT gene type is more likely to develop LVH.  

Key words: transient receptor potential channels, polymorphism, single nucleotide, gene frequency, essential hypertension；left ventricular hypertrophy；transient receptor potential-canonical 3 channels

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