天津医药

天津医药 ›› 2017, Vol. 45 ›› Issue (8): 817-821.doi: 10.11958/20170654

• 专题 颅脑神经创伤 • 上一篇    下一篇

N-乙酰半胱氨酸抑制急性脊髓损伤后炎症因子的 作用研究

江继鹏 1， 程远驰 1， 2， 刘宝虎 3， 边科砜 4， 逄瑷博 4， 陈旭义 1△   

  1. 1 天津， 武警后勤学院附属医院脑科中心， 武警部队脑创伤与神经疾病研究所， 天津市神经创伤修复重点实验室（邮编 300162）； 2 锦州医科大学； 3 天津中医药大学； 4 武警后勤学院学员二旅
  • 收稿日期:2017-06-09 修回日期:2017-07-15 出版日期:2017-08-15 发布日期:2017-08-15
  • 通讯作者: 江继鹏 E-mail:13502034360@163.com
  • 作者简介:江继鹏 （1992）， 男， 研究生在读， 主要从事颅脑创伤和常见神经系统疾病的诊疗研究
  • 基金资助:
    国家科技重点研发计划 （2016YFC1101504）； 国家自然科学基金面上项目 （11672332）

Inhibitory effects of N-acetylcysteine on inflammatory factors after acute spinal cord injury

JIANG Ji-peng1， CHENG Yuan-chi1,2， LIU Bao-hu3， BIAN Ke-feng4， PANG Ai-bo4， CHEN Xu-yi1△   

  1. 1 Institute of Brain Trauma and Neurology Disease of the Affiliated Hospital of Logistics University of People's Armed Police Forces, Tianjin 300162, China; 2 Jinzhou Medical University; 3Tianjin University of Traditional Chinese Medicine; 4 the Second Brigade, Logistics University of People's Armed Police Forces
  • Received:2017-06-09 Revised:2017-07-15 Published:2017-08-15 Online:2017-08-15

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摘要： 目的 探讨 N-乙酰半胱氨酸（NAC）抑制急性脊髓损伤后炎症因子的作用机制。方法 成年雌性 SD 大 鼠 54 只， 随机分为 3 组： 单纯椎板切除组（Sham 组）、 脊髓损伤组（SCI 组）、 N-乙酰半胱氨酸组（NAC 组）， 每组 18 只。Sham 组行 T9～10椎板切除术， 但不损伤脊髓； SCI 组和 NAC 组利用动脉瘤夹钳夹式法建立大鼠 T9~10脊髓损伤模 型； NAC 组于建立脊髓损伤模型后 15 min、 12 h 后分别经腹腔注射 NAC （150 mg/kg）。在造模后的第 24 h， 每组分别 取 12 只处死， 用苏木素-伊红 （HE） 染色观察脊髓组织损伤情况 （6 只）， 用酶联免疫吸附测定 （ELISA） 法测定各组脊 髓组织肿瘤坏死因子 （TNF） -α、 白细胞介素 （IL） -6 的含量 （6 只）。每组剩余的 6 只继续饲养至第 8 周， 其中 NAC 组 在第 1 周每日分别于早、 晚各进行 1 次 NAC 注射， 每次间隔 12 h， 连续 7 d。并分别于伤后 1、 2、 4、 6、 8 周对每组剩余 的 6 只大鼠行脊髓损伤行为学 （BBB） 评分和斜坡实验评价神经功能。结果 HE 染色结果显示， Sham 组脊髓形态完 整， 无出血及炎性细胞浸润； SCI 组脊髓形态、 炎症状况均较 NAC 组及 Sham 组严重。ELISA 结果显示， SCI 组及 NAC 组 TNF-α、 IL-6 含量较 Sham 组高， NAC 组明显少于 SCI 组 （P＜0.05）。BBB 评分和斜坡实验结果显示， SCI 组 和 NAC 组的 BBB 评分和斜坡角度大小较 Sham 组均明显降低（P＜0.05）， 并且 NAC 组结果相对于 SCI 组更好。结 论 NAC 可能通过降低大鼠脊髓损伤区组织中 TNF-α、 IL-6 的含量， 减轻脊髓局部炎症反应， 促进大鼠神经功能的 恢复。

关键词: 乙酰半胱氨酸, 脊髓损伤, 肿瘤坏死因子 α, 白细胞介素 6

Abstract: Objective To investigate the inhibitory effects of N- acetylcysteine (NAC) on inflammatory factors after acute spinal cord injury, and the mechanisms thereof. Methods A total of 54 clean and healthy adult female SD rats were divided into three groups according to the principle of randomization: simple laminectomy group (Sham group), spinal cord injury group (SCI group) and N-acetylcysteine group (NAC group), with 18 rats in each group. The Sham group was treated with T9- 10 laminectomy only without spinal cord injury. Aneurysm clamp was used to establish rat model of T9- 10 spinal cord injury in SCI group and NAC group. At the time of 15 min and 12 h after injury, the rats of NAC group were injected Nacetylcysteine intraperitoneally (150 mg/kg). At the time of 24 h post modeling, 12 rats were sacrificed in each group for observing the severity of tissue injury by using hematoxylin- eosin (HE) staining (6 rats), and detecting the contents of inflammation factors including tumor necrosis factor (TNF)- α and interleukin (IL)- 6 by using enzyme- linked immunosorbent assay (ELISA) (6 rats). The remaining 6 rats in each group were raised for 8 weeks. During the first week, the ones in NAC group were injected NAC twice a day at 12 h intervals for 7 d. Additionally, the neurological function evaluation was performed at week 1, week 2, week 4, week 6 and week 8 after injury in rats by using the spinal cord injury motor function score (BBB) and the inclined plate test. Results The results of HE staining showed that the spinal cord was intact without hemorrhage and inflammatory cell infiltration in Sham group. The morphology and inflammatory status were significantly worse in SCI group than those in NAC group and Sham group. The results of ELISA showed that the expressions of TNF-α and IL-6 were significantly higher in SCI group and NAC group than those in Sham group (P＜0.05), while the expression levels of TNF-α and IL-6 were significantly lower in NAC group than those of SCI group (P＜0.05). The BBB scores and inclined plate test showed that both were significantly lower in SCI group and NAC group than those of Sham group (P＜0.05), and the results were better in NAC group than those of SCI group. Conclusion NAC may promote the recovery of neurological function in rats by reducing the local inflammatory response through diminishing the contents of TNF-α and IL-6 in spinal cord.

Key words: acetylcysteine, spinal cord injuries, tumor necrosis factor-alpha, interleukin-6