富氢生理盐水对3期压力性损伤大鼠创面修复的影响

doi:10.11958/20260964

天津医药 ›› 2026, Vol. 54 ›› Issue (8): 802-807.doi: 10.11958/20260964

富氢生理盐水对3期压力性损伤大鼠创面修复的影响

刘冰雁¹^,²(), 黄冬雪¹^,², 张越¹, 刘海迎¹, 谢克亮¹, 庄淑梅², 沈悦好¹^,^△()

¹ 天津医科大学总医院重症医学科（邮编300052）
² 天津医科大学护理学院

收稿日期:2026-04-14 修回日期:2026-04-27 出版日期:2026-08-15 发布日期:2026-08-07
通讯作者: △E-mail：yuehaoshen@163.com
作者简介:刘冰雁（2002），女，硕士在读，主要从事重症护理方面研究。E-mail：bingyanliu123@163.com
基金资助:
天津市卫生健康科技项目重点学科专项(TJWJ2024XK006);天津市科技计划项目(25YFKFYS00890)

Effect of hydrogen-rich normal saline on wound repair in rats with stage 3 pressure injury

LIU Bingyan¹^,²(), HUANG Dongxue¹^,², ZHANG Yue¹, LIU Haiying¹, XIE Keliang¹, ZHUANG Shumei², SHEN Yuehao¹^,^△()

¹ Department of Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China
² School of Nursing, Tianjin Medical University

Received:2026-04-14 Revised:2026-04-27 Published:2026-08-15 Online:2026-08-07
Contact: △E-mail: yuehaoshen@163.com

刘冰雁, 黄冬雪, 张越, 刘海迎, 谢克亮, 庄淑梅, 沈悦好. 富氢生理盐水对3期压力性损伤大鼠创面修复的影响[J]. 天津医药, 2026, 54(8): 802-807.

LIU Bingyan, HUANG Dongxue, ZHANG Yue, LIU Haiying, XIE Keliang, ZHUANG Shumei, SHEN Yuehao. Effect of hydrogen-rich normal saline on wound repair in rats with stage 3 pressure injury[J]. Tianjin Medical Journal, 2026, 54(8): 802-807.

摘要/Abstract

摘要：

目的观察富氢生理盐水局部应用对3期压力性损伤大鼠创面修复的影响。方法选取36只SPF级SD雄性大鼠，建立3期压力性损伤模型后（其中4只用于评判建模是否成功），随机分为对照组与干预组，每组16只。对照组使用生理盐水清创，干预组使用富氢生理盐水（氢含量≥2 mg/L）清创，均2次/d。于干预第0、3、7、10、14天观察创面愈合率；第3天检测血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）及血管内皮生长因子（VEGF）水平；第7、14天取创面组织行HE、Masson染色及Ki-67免疫组化检测，评估组织病理、胶原沉积与细胞增殖情况。结果与对照组相比，干预组在第3、7、10、14天的创面愈合率均升高（P<0.05）；干预第3天，干预组血清TNF-α、IL-6水平降低，VEGF水平升高（P<0.05）。HE和Masson染色显示，干预组创面愈合质量更优，第7、14天胶原体积分数均高于对照组（P<0.05）。免疫组化显示，干预组第7天Ki-67阳性细胞比率高于对照组（P<0.05）。结论富氢生理盐水局部应用可促进3期压力性损伤创面愈合，其机制可能与减轻氧化应激与炎症反应、促进胶原合成与细胞增殖有关。

关键词: 再灌注损伤, 氧化性应激, 炎症, 压力性损伤, 富氢生理盐水, 创面修复

Abstract:

Objective To observe the effect of topical application of hydrogen-rich normal saline on wound repair in rats with stage 3 pressure injury. Methods Thirty-six SPF-grade male SD rats were selected to establish a stage 3 pressure injury model (among which 4 rats were used to evaluate successful modeling). Rats were then randomly divided into the control group and the intervention group, with 16 rats in each group. The control group received debridement with normal saline, while the intervention group received debridement with hydrogen-rich normal saline (with hydrogen content ≥ 2 mg/L), both twice daily. Wound healing rate was observed on the 0th, 3th, 7th, 10 and 14 days of intervention. On the day 3, serum levels of tumor necrosis factor-α (TNF-α)，interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) were measured. On days 7 and 14, wound tissue samples were collected for HE staining. Masson staining and Ki-67 immunohistochemistry were used to evaluate tissue pathology, collagen deposition and cell proliferation. Results Compared with the control group, the wound healing rate was significantly higher on days 3, 7, 10 and 14 in the intervention group (P<0.05). On days 3 of intervention, serum levels of TNF-α and IL-6 were decreased, while VEGF level was increased (P<0.05) in the intervention group. HE and Masson staining showed the wound healing quality was better in the intervention group, and the collagen volume fraction was higher on days 7 and 14 than those in the control group (P<0.05). Immunohistochemistry showed that the percentage of Ki-67 positive cells on days 7 was significantly increased in the intervention group than that of the control group (P<0.05). Conclusion Topical application of hydrogen-rich normal saline can promote wound healing in stage 3 pressure injury, and the mechanism may be associated with alleviating oxidative stress and inflammatory responses, as well as promoting collagen synthesis and cell proliferation.

Key words: reperfusion injury, oxidative stress, inflammation, pressure injury, hydrogen-rich saline, wound repair

中图分类号:

R318.01

图/表 8

表1 2组大鼠干预不同时间点创面愈合率比较

Tab.1 Comparison of wound healing rates at different time points between two groups of rats （n=4，%，$\bar{x}±s$）

组别	第3天	第7天	第10天	第14天
对照组	23.85±3.87	40.13±6.17	65.07±5.31	81.54±1.70
干预组	36.98±3.29	67.28±6.97	78.15±3.67	90.69±3.73
t	5.205^＊＊	5.893^＊＊	3.992^＊＊	4.654^＊＊

图1 2组大鼠不同时间点创面愈合情况图中标尺长度均为1 cm。

Fig.1 Wound healing status at different time points in two groups of rats

表2 2组大鼠TNF-α、IL-6、VEGF水平比较

Tab.2 Comparison of systemic inflammatory factors TNF-α，IL-6 and VEGF between two groups of rats （n=4，ng/L，$\bar{x}±s$）

组别	TNF-α	IL-6	VEGF
对照组	20.33±2.23	48.70±8.74	41.43±2.06
干预组	13.76±0.69	23.94±5.50	61.60±3.87
t	5.405^＊＊	4.925^＊＊	8.960^＊＊

图2 2组大鼠不同时间创面组织HE染色情况（×100）黑色箭头示再上皮化边缘，红色箭头示新生血管，黄色箭头示坏死组织。

Fig.2 HE staining of wound tissue at different time points in two groups of rats (×100)

表3 2组大鼠不同时间创面组织CVF比较

Tab.3 Comparison of CVF in wound tissue at different time points between two groups of rats （n=4，%，$\bar{x}±s$）

组别	第7天	第14天
对照组	33.92±2.65	54.92±9.24
干预组	48.84±6.66	75.31±8.19
t	4.009^＊	3.206^＊

图3 2组大鼠不同时间创面组织Masson染色情况（×100）

Fig.3 Masson staining of wound tissue at different time points in two groups of rats (×100)

表4 2组大鼠不同时间创面组织Ki-67阳性细胞比率比较

Tab.4 Comparison of Ki-67 positive cell ratio in wound tissue at different time points between two groups of rats （n=4，%，$\bar{x}±s$）

组别	第7天	第14天
对照组	28.16±1.70	22.72±2.84
干预组	34.57±3.88	25.87±4.02
t	2.966^＊	1.241

图4 2组大鼠不同时间创面组织免疫组化Ki-67染色情况（×200）

Fig.4 Immunohistochemical Ki-67 staining of wound tissue at different time points in two groups of rats (×200)

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富氢生理盐水对3期压力性损伤大鼠创面修复的影响

Effect of hydrogen-rich normal saline on wound repair in rats with stage 3 pressure injury

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