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IGF-1和 COX-2在子宫内膜癌中的表达及其临床意义

陈红漫   

  1. 天津医科大学广东路校区博士生公寓106
  • 收稿日期:2012-04-28 修回日期:2012-07-05 出版日期:2013-01-15 发布日期:2013-01-15
  • 通讯作者: 陈红漫

The expression and clinical significance of IGF-1 and COX-2 in endometrial cancer

Chen Hong Man   

  • Received:2012-04-28 Revised:2012-07-05 Published:2013-01-15 Online:2013-01-15
  • Contact: Chen Hong Man

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摘要： 【摘要】 目的检测胰岛素样生长因子(IGF)-1和环氧合酶(COX)-2在子宫内膜组织中的表达,分析其与临床病理特征的关系。方法 150例子宫内膜样腺癌组织(EA组)、54例非典型增生(AHE组)及84例正常子宫内膜组织(NE组)。应用免疫组化SP法检测IGF-1及COX-2在各组中的表达情况。结果 (1)EA组IGF-1和COX-2阳性表达率(70.0%、78.7%)高于NE组(45.2%、44.0%),差异有统计学意义(P<0.01)。(2)EA组IGF-1阳性表达在不同子宫肌层浸润间差异有统计学意义(P<0.05);COX-2阳性表达在不同病理分级间差异有统计学意义(P<0.05)。(3)IGF-1和COX-2在子宫内膜样腺癌中表达呈正相关(rs=0.183,P<0.05)。结论 IGF-1和COX-2在子宫内膜样腺癌中可能存在协同作用,同时检测有利于早期诊断和预测其分化浸润情况。

关键词: 子宫内膜肿瘤, IGF-1, COX-2, 免疫组织化学, 组织芯片

Abstract: Abstract Objective：With the aim to further investigate the etiology of endometrial cancer, tissue chip technology was used to evaluate the expression of Insulin-like growth factor-1(IGF-1) and cyclooxygenase-2（COX-2）in endometrial cancer tissues. Furthermore, the association between their expression and the clinicopathological features of this malignancy was also examined. Methods：The tissue chip contained 150 cases endometrial cancer examples, 54 atypical and 84 normal endometrial examples were also contained.Immunohistochemistry SP method was applied to evaluate the expression of IGF-1 and COX-2 in the tissue chip. Results：(1)The expression of IGF-1 and COX-2 in endometrial cancer were significantly higher than that in the normal examples（P<0.05）.(2)The expression of IGF-1 was positively associated with myometrial invasion（P<0.05）.No association was found between the expression of IGF-1 and histological grade, FIGO phase and lymph node metastasis. The expression of COX-2 was found positively associated with histological grade（P<0.05）.No association was found between the expression of COX-2 and myometrial invasion, FIGO phase and lymph node metastasis.(3)The effects of IGF-1 and COX-2 on the development of endometrial cancer were synergistic（P<0.05）. The expression of them was positively associated. Conclusion：With the tissue chip to evaluate the expression of IGF-1 and COX-2 in endometrial cancer ,help to earlier diagnosis of endometrial carcinoma and its pre-cancerous,comprehensive evaluation its biologic behavior, and predict invasion of biology indexes, to judge the degree malignant to have the important meaning.

Key words: endometrial carcinoma, IGF-1, COX-2, immunohistochemical method, tissuechip