天津医药

• 实验研究 •    

普罗布考对新西兰兔动脉粥样硬化的影响

唐雅玲1,黄韬2,杨永宗1,孙慧1,胡国晶1   

  1. 1. 南华大学心血管疾病研究所，动脉硬化学湖南省重点实验室
    2. 中国人民解放军95871部队
  • 收稿日期:2011-05-30 修回日期:2011-07-25 出版日期:2012-01-15 发布日期:2012-01-15
  • 通讯作者: 唐雅玲

Effect of Probucol on Atherosclerosis of New Zealand Rabbits

  • Received:2011-05-30 Revised:2011-07-25 Published:2012-01-15 Online:2012-01-15
  • Contact: Ya-Ling TANG

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摘要： 目的：研究普罗布考对正常饮食及高脂高胆固醇饮食新西兰兔血脂、肥大细胞及动脉粥样硬化的影响。方法：40只雄性新西兰兔分别给予基础饲料、基础饲料+普罗布考、高脂高胆固醇饲料、高脂高胆固醇饲料+普罗布考喂养4周，取材。采用酶比色法检测血清中脂质水平；甲苯胺蓝染色检测肥大细胞；苏丹Ⅳ染色测定主动脉粥样硬化病变面积。结果：与高脂高胆固醇组比较，高脂高胆固醇+普罗布考组新西兰兔血清三酰甘油（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）水平均明显降低（P<0.05）；主动脉和空肠脱颗粒的肥大细胞百分率明显减少（P<0.001）；主动脉粥样硬化病变面积明显减少（P<0.001）。结论：普罗布考能降低高脂高胆固醇饮食新西兰兔血清TG、TC、LDL-C和HDL-C水平；减少主动脉、空肠脱颗粒的肥大细胞百分率以及主动脉粥样硬化病变面积。

关键词: 普罗布考, 膳食, 致动脉粥样化, 动脉粥样硬化, 脂类, 肥大细胞, 主动脉, 兔

Abstract: Objective:To investigate effect of probucol on serum lipid, mast cells and atherosclerosis of New Zealand rabbits with normal diet or high fat /high cholesterol diet. Methods:Forty male New Zealand rabbits were respectively supplied with normal fodder, normal fodder and probucol, high fat /high cholesterol fodder, high fat /high cholesterol fodder and probucol, then observed for 4 weeks. The serum lipids were measured. Mast cells were detected by toluidine blue. Aorta atherosclerotic lesion area was measured with Sudan IV. Results:There was a significant decrease in serum triglyceride, total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol(P<0.05), the percentage of degranulated mast cells in aorta and in jejunum (P<0.001), aorta atherosclerotic lesion area (P<0.001) in high fat /high cholesterol fodder and probucol group than in high fat /high cholesterol fodder group. Conclusion:Probucol can decrease serum triglyceride, total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol, the percentage of degranulated mast cells in aorta and in jejunum, aorta atherosclerotic lesion area of New Zealand rabbits with high fat /high cholesterol diet.

Key words: probucoldiet, atherogenicatherosclerosislipidsmast cellsaortarabbits, 兔