Abstract: Abstract Objective study the effectiveness of internal structure and surface energy of Titanium (Ti) and Ti-6Al-7Nb (TC20) are treated by mechanical grinding and sandblasting etching on rat osteoblasts. Methods Pure titanium and Ti-6Al-7Nb were divided into experimental group and control group, control group directly mechanical polishing with sand paper, the experimental group uses large particles containing the Al203 and good ratio acid etching liquid to sandblasted and acid-etched corrosion two titanium surface, Then osteoblasts extracted from rats were seeded on titanium sheets,?low multiple microscope analyze titanium surface morphology, contact Angle measurement instrument analyze titanium surface hydrophily, the two groups by SLA were soaked into SBF for 7d，14d, 21d, then SEM and XRD observe surface sediment morphology and phase, observe different titanium surface adhesion of osteoblast cells by scanning electron microscope and analyze the proliferation of osteoblast by MTT test. Results Pure titanium and Ti-6Al-7Nb surface in mechanical grinding group showed scratches, in SLA group showed a large hole morphology and titanium surface is hydrophilic structure, samples soaked into SBF for 14d, Ti-6Al-7Nb surface firstly observed the hydroxyapatite coating, Ti and Ti-6Al-7Nb surface both observed the hydroxyapatite coating after 21d, a lot of mouse osteoblasts adhere in titanium surface with sandblasting acid corrosion processing, the proliferative ability of osteoblasts in sandblasting acid corrosion was stronger than smooth groups through the statistical analysis(P<0.05). Conclusion Ti-6Al-7Nb by SLA is advantageous to the osteoblast adhesion, hydroxyapatite coating is helpful to promote the combination of implant and bone tissue.

Key words: Key words: Titanium, Ti-6Al-7Nb, mechanical grinding, sandblasting etching, simulated body fluid, osteoblast