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15 March 2016, Volume 44 Issue 3 Previous Issue    Next Issue
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Cell and Molecular Biology

Cell and Molecular Biology



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The effects of Ti-6Al-7Nb surface by sanding acid etch treatment on rats osteoblast SUN Hongwan, LI Zhigang, ZHANG Jingying 2016, 44 (3):  257-260.  doi: 10.11958/20150084 Abstract ( 816 )   PDF (2132KB) ( 3791 )   Abstract Objective study the effectiveness of internal structure and surface energy of Titanium (Ti) and Ti-6Al-7Nb (TC20) are treated by mechanical grinding and sandblasting etching on rat osteoblasts. Methods Pure titanium and Ti-6Al-7Nb were divided into experimental group and control group, control group directly mechanical polishing with sand paper, the experimental group uses large particles containing the Al203 and good ratio acid etching liquid to sandblasted and acid-etched corrosion two titanium surface, Then osteoblasts extracted from rats were seeded on titanium sheets,?low multiple microscope analyze titanium surface morphology, contact Angle measurement instrument analyze titanium surface hydrophily, the two groups by SLA were soaked into SBF for 7d，14d, 21d, then SEM and XRD observe surface sediment morphology and phase, observe different titanium surface adhesion of osteoblast cells by scanning electron microscope and analyze the proliferation of osteoblast by MTT test. Results Pure titanium and Ti-6Al-7Nb surface in mechanical grinding group showed scratches, in SLA group showed a large hole morphology and titanium surface is hydrophilic structure, samples soaked into SBF for 14d, Ti-6Al-7Nb surface firstly observed the hydroxyapatite coating, Ti and Ti-6Al-7Nb surface both observed the hydroxyapatite coating after 21d, a lot of mouse osteoblasts adhere in titanium surface with sandblasting acid corrosion processing, the proliferative ability of osteoblasts in sandblasting acid corrosion was stronger than smooth groups through the statistical analysis(P<0.05). Conclusion Ti-6Al-7Nb by SLA is advantageous to the osteoblast adhesion, hydroxyapatite coating is helpful to promote the combination of implant and bone tissue. Related Articles | Metrics

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The changes of expressions of BMP-6 with PRF on mandibular distraction osteogenesis FU Ying, DONG Qingwen, WANG Zhiying 2016, 44 (3):  261-264.  doi: 10.11958/20150028 Abstract ( 799 )   PDF (639KB) ( 3765 )   Objective To investigate the effects of PRF on mandibular distraction osteogenesis and the expression of BMP-6 in the period of distraction osteogenesis. Methods Twenty-five mature rabbits were randomly divided into five groups, Mandibular osteotomies were performed corticectomy in bilateral mature rabbits,PRF were implanted in the one side of mandibles as experimental group. Another side of madibular as control group,The rabbits were sacrificed and the distracted calluses were harvested and processed for HE and BMP-6 immunohistochemistry on the 1st,3th,7th,14st and 28th day after the end of distraction, respectively.The expressions of BMP-6 in the distracted calluses were analyzed by cell digital imaging software. Results The regenerated bone was found in the distraction gap after mandibular lengthening.BMP-6 were co-localized in cytoplasm of osteoblasts and newly embedded osteocytes.Compared with control group, the positive cell rate and positive area percentage of BMP-6 were higher on the 1st, 3th and 7th day after the distraction in experimental group(P＜0.05).Then there was no statistical significance on the positive cell rate and positive area percentage of BMP-6 on the 14st and 28th day after the distraction in experimental group.Conclusion? PRF could accelerate bone formation of mandibular distraction osteogenesis in rabbit,BMP-6 may play important roles at the early stage of mandibular distraction. Related Articles | Metrics

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Analysis of C5 palsy after open door laminoplasty LI Huiming, XIA Gang, LIU Yang, TIAN Rong 2016, 44 (3):  265-267.  doi: 10.11958/20150180 Abstract ( 1166 )   PDF (505KB) ( 3882 )   Objective: To discuss the relationship of C5 never palsy incidence and difference risk factor after cervical open door laminoplasty. Methods： This study involved the patients with cervical spondylotic myelopathy who nuderwent expansive open door laminoplasty between January 2009 and December 2012. All the patients were average follow up (24.9 + 2.6) months. They were classified into two groups according to whether occur C5 palsy，group A ,20 cases occur C5 palsy and group B，234 cases without. Compare two groups of different risk factors and postoperative C5 palsy occurred, analyze the factors may affect the C5 palsy occurred. Results: All cases received follow-up (24.9 + 2.6) months on average, compared two groups of patients in postoperative changes of risk factors for postoperative CCI（t=10.015 P＜0.05）, lamina open angles (（t=5.125 P＜0.05）), spinal cord shift (t=10.159 P＜0.05), the difference is statistically significant. Preoperative intramedullary high signal 、OPLL（Ossification Of Posterior Longitudinal Ligament）、Intervertebral foramen stenosis differences without statistical significance. C5 palsy risk factors, according to the results of Logistic regression analysis of multiple factors in the Spinal cord shift, lamina open angles, postoperative CCI and statistical correlation between C5 palsy, OR（95%CI）:3.542（2.465~9.254）、2.035（1.684~2.359）、9.126（4.425~17.273）. Conclusion: The Angle of open door too large; CCI and spinal cord shift is risk factors for postoperative C5 palsy. Related Articles | Metrics

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Long term follow up of the spinal stenosis and symptoms after lamina decompression LIU Gang, LI Shuang, DENG Shucai，HAO Yonghong, JING Feng 2016, 44 (3):  268-270.  doi: 10.11958/20150170 Abstract ( 1083 )   PDF (271KB) ( 3874 )   Abstract: Objective: To study the relationship between the spinal instability and symptoms after lamina decompression. Method: Of 132 patients who accepted lamina decompression from 1990 through 2014, 76 were followed up for a minimum of 4 years. The patients were divided into instability group and non-instability group according to the X-ray result of the last following up. Compare with the VAS score, JOA score between the two group of the preoperation, 1 month after operation and the last follow up, and the JOA improvement rate index before and after the surgery, t test and chi square test method was used to analyze the outcome, with P < 0.05 a statistically significant difference. Results: Of the 76 patients, there were 27 patients in the instability group and 49 patients in the non-instability group which accounting for 35.5% and 64.5%. There were statistical significant in the VAS and JOA score between before and after surgery (P<0.05), but there were no statistical significant in the two groups(P >0.05). In the JOA improvement rate index, the non-instability group is higher than the instability group ,but no statistical significant(P>0.05). Conclusion: As a non-fusion method, there is no relationship between instability and symptoms improvement, which would be a better choice under the premise of surgical indications. Related Articles | Metrics

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Comparison of primary total hip arthroplasty in elderly patients performed with a minimally invasive direct lateral approach versus the standard lateral approach YUAN Xiaowei, LI Lin, LI Qingsong 2016, 44 (3):  271-273.  doi: 10.11958/20150138 Abstract ( 1088 )   PDF (300KB) ( 3884 )   Objective To compare the curative effects between minimally invasive and standard direct approach of pri⁃ mary total hip arthroplasty (THA) in elderly patients. Methods One hundred and eighteen geratic patients with hip disease were divided into minimally invasive group (n=61) and standard group (n=57). All the patients were followed up from 15 months to 3 years. The length of incision, surgical duration, intraoperative blood loss, postoperative drainage, number of blood transfusion, hospitalization time and the Harris hip scores were compared between two groups. Results The length of incision, intraoperative blood loss, postoperative drainage, number of blood transfusion and hospitalization time were signifi⁃ cantly lower in minimally invasive group than those in standard group, but the Harris hip scores were significantly higher 3 months after the operation in minimally invasive group than those in standard group (P < 0.05). First intention wound healing was found in all patients in two groups. The postoperative complications were significantly lower in minimally invasive group than those in standard group (3.28% vs 15.79%, P < 0.05). Conclusion Compared with standard incision of primary total hip arthroplasty , minimally invasive direct lateral approach has its advantages, including small surgical trauma, less hospitaliza⁃ tion time, fewer complications, and which can help restore joint function quickly, making the curative effect satisfied in elderly patients. Related Articles | Metrics

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A study of the diagnostic reliability of intertochanteric fracture classification LI Wanqiang, WU Dou, ZHENG Genqiang, MA Xiaolong, LIU Qiang 2016, 44 (3):  274-277.  doi: 10.11958/20160045 Abstract ( 1092 )   PDF (285KB) ( 4182 )   Objective To compare the diagnostic reliability of intertochanteric fracture AO and Evans-Jensen classifications in X ray film and three-dimensional CT reconstruction images and explore advantage of the three-dimensional CT. Methods A retrospective study was performed to evaluate 54 intertochanteric fractures．Thress orthopaedic surgeonswere asked to make assessment of fracture classifications using X ray film and the three-dimensional CT images. Agreement test was performed to evaluate interobserver and intraobserver reliability for fracture classification. Results When X ray film was used, interobserver reliability mean kappa value between three surgeons for AO and Evans-Jensen classifications were 0.597 (0.547-0.653) and 0.571 (0.539-0.600), medium consistency. While using three-dimensional CT to AO classification lower kappa value was 0.411 (0.366-0.455), medium consistency, Evans - Jensen classification kappa value was 0.704 (0.671-0.737), highly consistency. Each surgeon respectively through the X ray film and three-dimensional CT the AO and Evans-Jensen classification mean kappa value were 0.464 (0.406-0.579), medium consistency and 0.191 (0.160-0.233), weak consistency. Conclusion Both classifications based on X ray film showed moderate consistency , using three-dimensional CT does not improve the consistency of AO classification. And the three-dimensional CT does not apply to Evans-Jensen classification. Related Articles | Metrics

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Radiofrequency combined with ozone therapy in patients with discogenic low back pain XU Jin, MA Xinlong, SUN Xiaolei, ZHAO Zhihu 2016, 44 (3):  278-280.  doi: 10.11958/20160046 Abstract ( 885 )   PDF (294KB) ( 3844 )   Objective To investigate the clinical efficacy of radiofrequency surgical therapy combined with ozone for patients with discogenic low back pain. Methods The clinical data were collected in 120 patients treated with radiofrequency and ozone for lumbar disc herniation at Tianjin Hospital from October 2013 to October 2015. Pain visual analogue scale (VAS) was used to patients at preoperative and postoperative 1 week, 1, 3, 6 and 12 months. Health survey (SF-36) score was used at preoperative and postoperative six months. The efficacy was evaluated by MacNab curative effect evaluation. Re⁃ults The preoperative VAS score was (7.02±0.64) points. The postoperative VAS scores were (3.13±0.32) points, (2.11 ± 0.67) points， (2.62±0.89) points， (2.37±0.34) points and (2.31±0.50) points at one week, one month, three months, six months and twelve months, respectively. The VAS scores were significantly decreased after surgery (P < 0.05). Preoperative SF-36 score was (48.32 ± 7.46) points, which reached to the (82.03 ± 5.89) points six months after surgery (P < 0.05). After six months, the fineness rate reached to 89.17% evaluated by MacNab curative effect evaluation. Conclusion Radiofrequency combined with ozone treatment is an effective and reliable method for discogenic low back pain. Related Articles | Metrics

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Research progress of the association between Pro-inflammatory cytokines and Osteoarthritis LI Bing, LIU Jun, XIAO Yu, BU Yanmin, XING Dan 2016, 44 (3):  281-285.  doi: 10.11958/20150380 Abstract ( 873 )   PDF (422KB) ( 4686 )   Osteoarthritis (OA) is the main reason of pain and disability of joint for old people in China, and its incidence is increasing year by year. Besides proliferation of osteophytes and degeneration of cartilage, inflammation, as one of the dominant pathological changes in OA, is causing wider attention. Pro-inflammatory cytokines (PIC) are important mediators of inflammation. The increased level of PIC in OA can lead to systemic and local inflammation, result in further destruction of many kinds of tissues in the joint (such as cartilage), and accelerate the development of OA. Besides, the level of inflammation is closely related to the clinical symptoms of OA. Therefore, it is important to understand the role of PIC in the pathogenesis of OA. This article reviewed the association, as well as the molecular mechanism, between PIC and OA, providing new concepts for diagnose and treatment of OA. Related Articles | Metrics

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Research progress of in-vivo kinematics after cervical arthrodesis LIU Jia′nan， XIA Qun， LI Hongda， WEI Dong 2016, 44 (3):  286-289.  doi: 10.11958/20150317 Abstract ( 1058 )   PDF (301KB) ( 3597 )   To review the researches of in-vivo kinematics after cervical arthrodesis. Related literature concerning the kinematics in patients after cervical arthrodesis was extensively reviewed and comprehensively analyzed in 4 terms of changes in adjacent segment range of motion , motion segment percent contributions, motion pattern of cervical facet joints, and deviated center of rotation at adjacent segments. These reported researches of in-vivo kinematics after cervical arthrodesis was almost on the sagittal plane. Few data have been reported on the 6-DOF kinematics under physiological loading conditions. Whether adjacent segment pathology caused by hypermobility remains controversial. Long-term follow-up of large sample randomized controlled studies and obtaining the accurate 6-DOF kinematics are the best way to resolve controversy. Related Articles | Metrics

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The effect of moderate static magnetic fields on secretion of pro-inflammatory factors in THP-1 cells GUO Zhixia, MAO Libin, WANG Huiqin, ZHANG Rui, ZHANG Tongcun 2016, 44 (3):  290-293.  doi: 10.11958/20150055 Abstract ( 801 )   PDF (348KB) ( 3747 )   Abstract: Objective To investigate the effect of moderate static magnetic fields (SMF) on secretion of inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) in human monocytic leukemic cell line THP- 1. Methods THP- 1 cells at logarithmic phase were divided into control group and magnetic treatment group. CCK-8 method was used to detect cell proliferation after THP-1 cells were exposed to 60 mT, 200 mT and 400 mT static magnetic fields at 18, 24 and 48 h. Then THP-1 cells were divided into control group, magnetic treatment group, LPS activation group and LPS+SMF treatment group. When magnetic treatment group and LPS+SMF treatment group were exposed to SMF at 18, 24 and 48 h, the levels of the cytokines TNF-α, IL-6 and IL-8 were determined by ELISA. Results (1)60 mT, 200 mT and 400 mT SMF had no significant effects on cell proliferation in THP-1 cells (P > 0.05). (2)THP-1 cells secreted more TNF-α and IL-6 in 24 h than 18 h in every group, while IL-8 didn′t change. Compared with 24 h, the secretion of TNF-α decreased and IL-6 didn′t change, while IL-8 increased in 48 h. At three sampled time THP-1 cells of LPS activation group secreted more TNF-α, IL-6, IL-8 than those of control group and magnetic treatment group. After magnetic treatment THP-1 cells of LPS+SMF treatment group secreted less TNF-α, IL-6, IL-8 than those of LPS activation group (P < 0.05). Conclusion Static magnetic field may have some inhibitory effects on release of TNF-α, IL-6, IL-8 from THP 1 cells, which can provide basic data for the treatment of rheumatoid arthritis. Related Articles | Metrics

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Effect of cobalt chloride-induced hypoxia on cell migration, expression and secretion of FSTL1 in melanoma cell lines REN Fangyuan， LI Lian， JIANG Fangxin， FENG Jing， CHEN Baoyuan,CAO Jie 2016, 44 (3):  294-297.  doi: 10.11958/20150144 Abstract ( 941 )   PDF (410KB) ( 3652 )   Objective The aim of this study was to explore the effect of cobalt chloride-induced hypoxia on migration of melanoma cells, and detect the transcription, expression and secretion of FSTL1 in this process. Methods B16F10 melanoma cell lines were treated with CoCl2 in order to mimic hypoxia. Experiment was divided into three groups: 0 μmol/L,50 μmol/L ,100 μmol/L .MTT assay was used to assure cell viability and determine the treatment concentration of CoCl2. Transwell assay was used to determine the migration ability of B16F10 melanoma cell line. Real-time PCR was used to measure the mRNA expression of FSTL1. Western blot were used to detect the protein expression of intracellular and extracellular of FSTL1. Results The cell viability of B16F10 melanoma cell lines was significantly reduced by CoCl2 treatment, in a time and concentration-dependent manner; The migration ability of B16F10 cell lines in CoCl2 treated group was significantly increased compared with the control group（all P<0.05）; The mRNA level of Fstl1 in CoCl2 treated group were obviously higher than the control group （all P<0.05）, the same as the protein of FSTL1. Simultaneously, the extracellular protein level of FSTL1 was significantly decreased compared with the control group and even it was not found in 100umol/L CoCl2 treatment. Conclusion The migration ability of melanoma cell lines was enhanced by CoCl2 treatment, which may be associated with expression and secretion of FSTL1, however, the relevant mechanism still be explored in the future. Related Articles | Metrics

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In vitro and in vivo pharmacodynamic screening of anticancer agents as c-Met inhibitor WANG Ying, ZHANG Yuhua, CHEN Zhanfa, LIU Yugang 2016, 44 (3):  298-301.  doi: 10.11958/20150078 Abstract ( 826 )   PDF (373KB) ( 4217 )   Objective To screen 8 series of LY compounds, which are c-Met tyrosine kinase inhibitors, and evaluate their anti-tumor effect in vitro and in vivo. Methods Preliminary screening was carried out by detecting the c-Met kinase phosphorylation inhibition activity of the compounds. CCK8 assay was adopted for secondary anti-tumor screen of the selected compounds using MKN-45, U87MG, Caki-1, PC-3 cell lines in vitro. The sc transplanted tumor model of U87MG cells in nude mice was established to evaluate the antitumor activity in vivo. Results Four compounds (LY22, LY25, LY28, LY32) with better activities were selected by HTRF method, where LY28 had better inhibitory effect on c-Met than Crizotinib. The above active compounds showed different degrees of inhibition on four kinds of target cells (MKN-45, U87MG, Caki-1 and PC-3) by CCK8 method, and the inhibition of LY28 was the most obvious. Antitumor activity in vivo showed that LY28 could significantly inhibited tumors growth in a dose-dependent manner. The tumors inhibition rate in high-dose of LY28 was 78.13%, close to 80.47% in Crizotinib group. Conclusion The compound LY28 has good antitumor activity in vitro and in vivo, which will be a new tyrosine kinase inhibitor. Related Articles | Metrics

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Pulsatill Pulsatilla saponin D combined with sorafenib against hepatocellular carcinoma cell invasion and metastasis of HE Wubin, SU Rongjian 2016, 44 (3):  302-305.  doi: 10.11958/59140 Abstract ( 788 )   PDF (481KB) ( 3788 )   [Abstract] Objective To investigate Pulsatilla saponin D joint sorafenib on human hepatoma cell line invasion and metastasis of influence. Methods Human hepatocellular carcinoma cell BEL-7402 into Pulsatilla saponin D single-drug treatment group to compare three treatments sorafenib monotherapy treatment group and the two-drug combination treatment group, observation methods on liver cancer cell invasion and metastasis.Results detect the adhesion inhibition rate in each group, the role of 3h, sorafenib group was significantly higher than Pulsatilla saponin D group (P <0.05 or <0.01), the combined treatment group was significantly higher than the sorafenib group and Pulsatilla saponin D group ( P <0.01); the role of 5h, Pulsatilla saponin D group was significantly higher than the adhesion inhibition sorafenib group (P <0.01), and higher than Pulsatilla saponin D group action 3h (P <0.01), described the two drugs on liver cancer cell adhesion synergistic inhibition rate (Q = 2.33> 1.15); detecting migration inhibitory rate in each group, sorafenib monotherapy group and the two-drug combination group of hepatoma cell migration inhibition were significantly higher than Pulsatilla saponin D monotherapy group (P <0.01), and the inhibition rate of two-drug combination group was significantly higher than that of sorafenib monotherapy group (P <0.01), described the two-drug combination migration of hepatoma cell line has a synergistic effect (Q = 1.39> 1.15); Detection of invasion inhibition rate, sorafenib monotherapy group and the two-drug combination group were significantly higher than Pulsatilla saponin D group (P <0.01), described the two drugs on liver cancer cell invasion inhibition of antagonistic effect (Q = 0.68 <0.85). Each group of VEGF-C, MMP-9 expression: MMP-9 expression levels Pulsatilla saponin D monotherapy significantly decreased sorafenib group and two-drug combination group VEGF-C, MMP-9 expression was significantly decreased (P <0.05); compared with Pulsatilla saponin D monotherapy group, VEGF-C expression levels of the two-drug combination group was significantly decreased; two-drug combination group Baitouweng saponin D monotherapy VEGF-C level decreased significantly (P <0.05). Conclusion Pulsatilla saponin D, sorafenib monotherapy as well as combination of two drugs on liver cancer cells BEL-7402 cell line adhesion, migration, invasion has a certain extent, and both hepatoma cell adhesion, migration inhibitory synergistic . Related Articles | Metrics

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Sulforaphane suppressed the proliferation of glioma stem cells via miR-124 XIE Youke, LI Xuemei, HUANG Dingping 2016, 44 (3):  306-310.  doi: 10.11958/20150197 Abstract ( 894 )   PDF (382KB) ( 3719 )   Abstract: Objective To investigate the effect of sulfuraphane (SFN) on proliferation of glioma stem cell line SWO 38, and its mechanism threreof. Methods Cell proliferation of SWO-38 treated with SFN was measured by cell proliferation assay. Clone formation experiment, tumor sphere formation experiment and Western blotting method were applied to detect the ability of cell clone formation and tumor sphere formation, and the expression of stemness relative genes, such as β-catenin, Oct4, Sox-2 and c-Myc. The effects of SFN and/or miR-124 inhibitor (miR-124i) on the expression of stemness relative genes were compared. Changes of miRNAs (miRNA-9, 21, 221, 124, 128 and 181) induced by SFN were measured by real time quantity PCR. Results SFN suppressed the proliferation of SWO-38 cells in a dose-dependent manner, in which IC50 was (26.41±2.13) μmol/L. SFN also decreased the ability of forming cell clone and tumor sphere, as well as the expression of stemness relative genes (β-catenin, Oct4, Sox-2 and c-Myc) in a dose-dependent manner. At the same time, SFN led to the change in many miRNAs, during which SFN increased the transcription of miR- 124 (-5.9-fold) and miR-128 (-2.6-fold), and decreased the transcription of miR-9, miR-21 and miR-221. Compared to the blank control, the expression levels of β-catenin, Oct4, and Sox-2 were significantly increased in miR-124i group. On the other hand, the expressions of above genes were also higher in combined group, which was treated with miR-124i and SFN than those in SFN group, but lower than those of miR-124i group. Conclusion SFN can efficiently inhibit the proliferation of giloma stem cells in SWO 38 cell line through miR-124/(β-catenin/Sox-2/Oct4) signaling pathway Related Articles | Metrics

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Neuroprotective effects of Blonanserin against H2O2-induced injuried in PC12 cells HUANG Haichao, WU Wei, ZHANG Xiaohong, NIE Yang, LIU Jingliang, ZHOU Jie 2016, 44 (3):  311-313.  doi: 10.11958/20150159 Abstract ( 893 )   PDF (410KB) ( 3713 )   To explore neuroprotective effects of blonanserin through PC12 cells injury model induced by H2O2. METHODS Used PC12 cells treated with H2O2 as oxidative stress damage cell model. PC12 cells were divided into 4 groups: control group(C group), H2O2-treated group(H group), blonanserin treated group(B group) and positive control group(vitamin E- pretreated, E group). The viability of PC12 cells injured by H2O2 was determined by MTT assay. Used hoechst33258 stained to detect the cell apoptosis and used TUNEL assay to detect the apoptotic rate. The SOD viability and MDA levels were detecded by biochemical methods. RESULTS Comparing with the C group, the apoptotic rate and MDA levels were increased in group H (P＜0.05) , while the cell viability and the SOD viability were decreased obviously(P＜0.05). Compared with H group, the cell viability, SOD viability were significantly increased(p＜0.05), while the MDA levels and apoptotic rate were decreased(P＜0.05). CONCLUSIONS The appropriate concentration of blonanserin showed neuroprotective effect on H2O2-induced PC cells injured. Related Articles | Metrics

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The difference of radiosensitivity between hematopoietic stem and progenitor cells toward difference dose of radiation LU Lu1, LI Deguan1, ZHANG Junling1, WANG Yueying1, MENG Aimin2△ 2016, 44 (3):  314-317.  doi: 10.11958/20150115 Abstract ( 881 )   PDF (464KB) ( 3814 )   Abstract: Objective To observe the difference of radiosensitivity between hematopoietic stem and progenitor cells toward difference dose of 137Csγ--ray irradiation. Methods Seventy-two C57BL/6 mice were randomly divided into control group and irradiated groups (n=18 for each). Mice of control group received sham irradiation, and the rest accepted 2、4 and 6Gy total body irradiation respectively, dose rate 1.01Gy/min. After irradiation 14 day、35day and 56day, the peripheral bloods were collected by balls enucleation. The number of bone marrow mononuclear cells、hematopoietic stem and progenitor cells were counted. Results Peripheral blood of irradiated mice had significant changes in the number of white blood cells and platelets in a dose-response relationship; at 14d post-radiation compared with the control group, the number of bone marrow mononuclear cells (BMMNCs) was decreased 21.9%、39.9% and 54.4%（t=4.311、6.401、8.007，P<0.05）；the number of BMMNCs and hematopoietic progenitor cells (HPCs) were significantly lower at 35d and 56d after 2Gy、4Gy and 6Gy irradiation（t=4.185、3.596，P<0.05）； the number of bone marrow hematopoietic stem cells in the irradiated group were lower than that in the control group at 14d、35d and 56d （t=9.706、3.427~7.465，P<0.05）. Conclusions 137Cs γ-ray irradiation on mouse hematopoietic system has some damage, the radiosensitivity of HPC is markedly higher than that of the HSC, where the difference ; the damage caused by radiation to hematopoietic stem cells is persistent. Related Articles | Metrics

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The differential effects of traditional Chinese medicine Danchaiheji on dendritic cells LI Yingxi1, CHEN Dan2, WANG Xiaodong2, JING Yaqing1, LI Keqiu1, LI Guang1△ 2016, 44 (3):  318-321.  doi: 10.11958/20150255 Abstract ( 1218 )   PDF (519KB) ( 4161 )   Objective To explore the effects of traditional Chinese formula Danchaiheji on the differentiation of regulatory dendritic cells (DCregs) and the underlying mechanism. Methods The rats’ serum with or without the treatment of formula Danchaiheji was prepared. The peripheral blood mononuclear cells were separated from the peripheral venous blood of healthy donors. CD14+ monocytes were isolated using CD14+ magnetic beads and cultured for 5–7 days to obtain immature dendritic cells (imDCs). Then the cells was divided into 4 groups, including the group of Danchaiheji containing rats’ serum after treatment of drug in the presence or absence of lipopolysaccharide (LPS) and the control group containing equal column rats’ serum in the presence or absence of LPS. Then the surface markers CD86, CD11b and HLA-DR of DCs were detected by flow cytometry and the levels of IL-10 were determined by enzyme-linked immunosorbent assays (ELISA). The proliferation of allogeneic T-cells was detected by flow cytometry and the expression level of indoleamine 2,3-dioxygenase (IDO) was determined using quantitative real-time PCR. Results DCs treated with the formula Danchaiheji exhibited high CD11b and low CD86 and HLA-DR expression levels as well as promoted the secretion of IL-10. In addition, the drug could inhibit the promotion of DCs on the proliferation of T cells, which is associated with the upregulation of IDO expression. Conclusion The traditional Chinese formula Danchaiheji could program DCs towards a regulatory DCs fate and the inhibitory effect on immune function was found. Related Articles | Metrics

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The studying of Fosinopril combined with Fenofibrate on the preventing of Diabetic retinopathy in diabetic mice KUANG Jinsong1, ZHAO Yurong1, ZHAO Yuyan2, Li Ruoxi1△, Xin Caihong1 2016, 44 (3):  322-326.  doi: 10.11958/59086 Abstract ( 1180 )   PDF (630KB) ( 3812 )   【Objective】 Studying the mechanism of the Fosinopril combined with Fenofibrate on the preventing of diabetic retinopathy and detecting the apoptosis and apoptosis reletive gene in the level of mRNA in the retinopathsis tissue ,the VEGF oxidizing material in the retina.【Method】 150 viripotent ICR mice(100 male mice,50 female mice) were randomly divided into five groups,every group included 30 mice. A group(Sham group, common forage given,Sodium Chloride injected),B group(Model group,exposure to high-fat diet for 4 weeks,mice were injected intraperitoneally with STZ,then lavaged with Sodium Chloride for 8 weeks),C group(Fosinopril preventing group, exposure to high-fat diet for 4 weeks,mice were injected intraperitoneally with STZ,then lavaged with fosinopril for 8 weeks),D group(Fenofibrate preventing group, exposure to high-fat diet for 4 weeks,mice were injected intraperitoneally with STZ,then lavaged with fenofibrate for 8 weeks),E group(Fosinopril combined with Fenofibrate preventing group,exposure to high-fat diet for 4 weeks,mice were injected intraperitoneally with STZ,then lavaged with fosinopril combined with fenofibrate for 8 weeks), Drawing the retina to detecing the activity of GSH-PX,SOD,ROS and the concentration of MDA and VEGF,then the mice were euthanized then detected the gene of Bax and Bcl-2 in the level of mRNA through the RT-PCR and detected the apoptosis of retinal cells through the Tunel staining method in the eyes.【Results】The BG of A group is lower than that of B,C,D ,E group(p<0.05)and which of B,C,D,E group are similar(p>0.05);A group expressed more Bcl-2 in the level of mRNA and the activity of GSH-PX,SOD were higher than other four groups(P＜0.05),but the concentration of ROS,MDA,VEGF,the expression of Bax in the level of mRNA and the Tunel index were lower than the B ,C, D groups(P＜0.05);B group expressed less Bcl-2 in the level of mRNA and the activity of GSH-PX,SOD were lower than other four groups(P＜0.05),but the concentration of ROS,MDA,VEGF,the expression of Bax in the level of mRNA and the Tunel index were higher than other four groups(P＜0.05);E group expressed less Bcl-2 in the level of mRNA and the activity of GSH-PX,SOD were lower than other C,D groups(P＜0.05),but the concentration of ROS,MDA,,the expression of Bax in the level of mRNA and the Tunel index were higher than C,D groups(P＜0.05);D group expressed more Bcl-2 in the level of mRNA and the activity of GSH-PX,SOD were higher than C group(P＜0.05),but the concentration of ROS,MDA,the expression of Bax in the level of mRNA and the Tunel index were lower than C group(P＜0.05);The concentration of VEGF in the Cgroup was lower than D group(P＜0.05).【Conclusions】 Fosinopril and Fenofibrate can improve the DR through inhibit the apoptosis and oxidation can conservate the retina,but Fosinopril combinated with Fenofibrate can get the better effect. Related Articles | Metrics

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The effect of renal denervation on hypothalamus angiotensin II and oxidative stress in myocardial infarction dogs MA Yijie, LU Chengzhi, LI Chao, ZHANG Jin, WANG Li, SONG Lijun 2016, 44 (3):  327-329.  doi: 10.11958/20150032 Abstract ( 748 )   PDF (299KB) ( 3812 )   Abstract: Objective To explore the effect of renal denervation (RDN) on hypothalamus angiotensin II and oxidative stress in myocardial infarction(MI) dogs. Methods Eighteen mongrel dogs were randomly divided into MI group (n=6), RDN group (n=6) and Sham operation group (n=6). Myocardial infarction model were made in the former two groups by gelatin sponge embolization of the left anterior descending artery. One week after MI, RDN was given to dogs in RDN group. Levels of hypothalamus angiotensin Ⅱ (AngⅡ), malondialdehyde (MDA), superoxide dismutase (SOD) and expression of gp91phox protein were detected four weeks after MI. Results Compared with control group, hypothalamus AngⅡ, MDA and expression of gp91phox protein were increased in MI group (P<0.01), but SOD was decreased (P<0.01). There was a positive correlation between AngⅡ and expression of gp91phox protein (rs =0.950, P<0.01) and negative correlation between AngⅡ and SOD (rs=-0.849, P<0.01) in MI group. Compared with MI group, hypothalamus AngⅡ, MDA and expression of gp91phox protein were decreased in RDN group (P<0.01),but SOD was increased (P<0.01).Conclusion RDN can decrease the level of hypothalamus AngⅡ and the level of hypothalamus oxidative stress , improve heart function of MI dogs. Related Articles | Metrics

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The investigation on the inhibitive effect of Berberine on gene expression of FSP27 to improve visceral white adipose tissues insulin resistance in type 2 diabetic hamsters LI Guosheng, LIU Xuhan, LI Xinyu, GAO Zhengnan, HUANG Lan, LIU Yali 2016, 44 (3):  330-335.  doi: 10.11958/20150041 Abstract ( 969 )   PDF (508KB) ( 3625 )   Abstract: Objective To study the effects of berberine (BBR) on the gene mRNA expression of fat-specific protein 27 (FSP27) and PR domain containing 16 (PRDM16) signal pathway in visceral white adipose tissues (VWAT) from type 2 diabetic (T2DM) Chinese hamsters and explore the related mechanisms. Methods The obese insulin-resistant (OIR) hamster models were induced by high-fat diet and T2DM hamster models were created by OIR hamster models injected with low-dose streptozotocin, the control group hamsters were fed with standard laboratory chow. After the induction, the hamsters were randomly divided into control, OIR, obese T2DM and BBR-treated T2DM groups. After nine-week BBR treatment, real-time quantitative PCR was used to measure the gene mRNA expression changes of VWAT FSP27 and PRDM16 signal pathway and their target genes from different groups. Results Compared with control group, the gene mRNA expression of PRDM16, CtBP-1, CtBP-2, C/EBPβ, PPARγ, PGC1α, PGC-1β and brown adipose tissue-specific genes such as UCP-1, Cidea, Elovl3, PPARα, and Acox, Cpt1 and Acadm was decreased and that of FSP27 and white adipose tissue-specific genes including Resistin、MEST and Serpina3k was increased in VWAT from hamsters of OIR and obese T2DM groups. BBR treatment down-regulated FSP27 expression, enhanced PRDM16 signal pathway, and induced the gene mRNA expression of brown adipose tissue-specific genes in VWAT from obese T2DM group to develop browning gene phenotype of visceral white adipose tissues, and then improved fat-induced insulin resistance. Conclusion Decreased VWAT FSP27 expression and increased VWAT PRDM16 expression involved in the molecular mechanisms of browning of visceral white adipose tissues induced by BBR, and contributed to improve abnormal lipids metabolism and fat-induced insulin resistance in VWAT by enhancing consumption of energy as heat to restore VWAT function. Related Articles | Metrics

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Study on the relationship between expression of miRNAs and receptors of epidermal growth factor in lung adenocarcinoma of non-smoking female WANG Fumin1, 2, ZHANG Huan1, XU Fangxiu1, QU Jinli1, KONG Jinyu1, QIAN Biyun 2016, 44 (3):  336-341.  doi: 10.11958/20150331 Abstract ( 765 )   PDF (703KB) ( 3961 )   Objective To determine association between the expression of miR-155, miR-16, miR-25 and miR-133 and epidermal growth factor receptor (EGFR) in lung adenocarcinoma (ADC) samples of non-smoking female, and their clini⁃ cal significance thereof. Methods According to EGFR types, 112 non-smoking female ADC patients were divided into two groups: EGFR wild (n=51) and EGFR mutant (n=61) groups. Expression levels of four miRNAs were detected by real time quantitative PCR. T-test was used to compare differences of four miRNAs expression in two groups. ADC patients were divid⁃ ed into high miRNA expression group and low expression group according to the mean value of miRNAs expression. Logrank test was used to compare the survival difference between the two subgroups. Cox proportional hazards regression analy⁃ sis was performed to determine the association between different ages (≤60， >60), EGFR types, miRNAs expression and over⁃ all survival. Results The expression of miR-25 was significantly higher in EGFR mutant group than that in EGFR wild group (P < 0.05). There were no significant differences in the survival rates of patients with different ages, EGFR types, and the expressions of miR-155, miR-16 and miR-133a. Patients with the lower expression of miR-25 had the higher survival time than patients with the higher expression of miR-25 (P < 0.05). In EGFR mutation group, there was no relationship be⁃ tween four miRNAs and the prognosis of ADC (P > 0.05). In EGFR wild group, the lower expression of miR-16, miR-25 or miR-133a was associated with longer survival in ADC (P < 0.05). Cox proportional hazards regression analysis revealed that the high expression of miR-25 was an independent risk factor for non-smoking female patients with ADC (P < 0.05). Con⁃ clusion miR-25 is an independent prognostic indicator for non-smoking female patients with ADC. High expression of miR-25 is found in non-smoking female patients with ADC, particularly in patients with EGFR wild-type, which may sug⁃ gest a poor prognosis. Related Articles | Metrics

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Effects of different concentrations of sevoflurane inhalation combined with propofol anesthesia on rocuronium pharmacodynamics YU Kai， WANG Guolin， YAN Donglai 2016, 44 (3):  341-344.  doi: 10.11958/20150132 Abstract ( 925 )   PDF (423KB) ( 3853 )   Objective To investigate the effect of different concentrations of sevoflurane inhalation combined with pro⁃ pofol anesthesia on rocuronium pharmacodynamics. Methods Sixty-seven patients, who underwent elective abdominal op⁃ eration in Tianjin Medical University General Hospital from Nov. 2014 to Feb. 2015, were randomly allocated to three groups: propofol combined 0.5 minimum alveolar concentration (MAC) end-tidal concentration of sevoflurane (group Ⅰ , 24 cases), propofol combined 0.75 MAC end-tidal concentration of sevoflurane (group Ⅱ , 20 cases) and propofol combined 1 MAC end-tidal concentration of sevoflurane (group Ⅲ, 23 cases). All those patients were given midazolam 0.05 mg/kg, sufen⁃tanil 0.3 μg/kg, etomidate 0.3 mg/kg for anesthesia induction. Rocuronium was given through the T1 mode of Closed-loop muscle relaxant infusion system and infused by 2ED95（0.6 mg/kg). The following variables were recorded: average consump⁃ tion dosage of rocuronium, recovery index, averaged consumption dosage of propofol and remifentanil. Results The aver⁃ aged consumption dosage of rocuronium was decreased in the three groups in turn[（9.71±2.38 vs 7.50±0.98 vs 6.90±1.14） μg· kg-1· min-1， F=18.562， P < 0.05]. There was no significant difference in recovery index between the three groups [（8.92± 2.62 vs 8.95±2.58 vs 10.30±3.65） min， F=1.577, P > 0.05]. The average consumption dosage of propofol and remifentanil were lower in group Ⅲ than those of group Ⅰ and group Ⅱ (P < 0.05). Conclusion High concentration of sevoflurane can enhance neuromuscular blockage effect of rocuronium, and decrease the consumption dosage of propofol and remifentanil Related Articles | Metrics

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The relationship between advanced fibrosis and diabetic neuropathy XIE Chunxiao1, JIA Guoyu2, WANG Lu2, LI Qiang2, WANG Shaocheng2, YANG Ling1, DI Fusheng2△ 2016, 44 (3):  345-348.  doi: 10.11958/20150105 Abstract ( 913 )   PDF (320KB) ( 4049 )   Abstract: Objective To investigate the relationship between advanced fibrosis and peripheral neuropathy in patients with type 2 diabetes mellitus (T2DM). Methods Select 173 patients (88 men and 85 women) with type 2 diabetes who hospitalized in tianjin third central hospital within nearly three years（2013.02-2015.02）and divide the participants into 3 groups according to NAFLD fibrosis score: group A (Low NFS，NFS≤—1.455), group B (Intermediate NFS，—1.455＜NFS＜0.676) and group C (High NFS，NFS≥0.676). Compare the prevalence of DPN between three groups. Diabetic neuropathy was ascertained by electroneurogram and liver fibrosis severity was ascertained by NAFLD fibrosis score. Results The prevalence of DPN in group A, group B and group C was 69.4%, 78.9% 86.2%, respectively. The prevalence of DPN was significantly higher in group C compared with group A (OR=5.96, P=0.014); there was no significant statistical difference between group B and group A (OR=1.76, P=0.155) in spite of that the prevalence of DPN was higher in group B than group A. Moreover, the prevalence of SNCA and MNCA were significantly higher in group C than group A (OR=6.50 vs 5.96, all P＜0.05). After adjustment for confounding factors, such as age, diabetic duration, HOMA-IR, serum lipid level, liver function and inflammatory factors, advanced fibrosis remained significantly associated with a higher odds ratio of DPN and MNCA(OR=2.16 vs 2.28, all P＜0.05), but no longer with SNCA(OR=1.88，P=0.087). Conclusion Advanced fibrosis has a close relationship with diabetic neuropathy, especially with motor nerve conduction abnormalities. Related Articles | Metrics

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The expressions of PD-L1 and PD-1 in peripheral T-cell lymphoma and their clinical significance ZHAO Yue, BU Qing 2016, 44 (3):  349-352.  doi: 10.11958/20150169 Abstract ( 1881 )   PDF (528KB) ( 3977 )   Objective To investigate the expression levels and clinical significance of PD-L1 and PD-1 in peripheral T-cell lymphoma.Methods Expression levels of PD-L1 and PD-1 in peripheral T-cell lymphoma tissues (test group, n=51) and lymph node optimum pathology (control group, n=20) were detected using immunohistochemistry. Correlations of PD-L1 and PD-1 expressions with clinical pathological parameters and prognosis were analyzed between two groups. Results The expression level of PD-L1 was significantly higher in test group than that in control group（74.51%vs35.00%，?2=9.662，P?0.05）.The expression level of PD-1 was significantly higher in test group than that in control group(66.67%vs 25.00%，?2=10.074，P?0.05).There were significant differences between PD-L1 and PD-1 expressions and peripheral LDH levels involved within test group. After two cycles of CHOP or ECHOP treatments,the response rate(RR) in PD-L1 negative group was higher than that in positive group（84.6%vs47.4%，?2=5.478, P?0.05）and the response rate (RR) in PD-1 negative group was higher than that in positive group（82.4% vs 44.1%，?2=6.755, P?0.05）. The media OS in PD-L1 in negative group was higher than that in positive group（29.8M vs 17.6M，P?0.05）and the media OS in PD-1 negative group was higher than that in positive group（29.8M vs 17.6M，P?0.05）.Conclusion：There was a high level expression of PD-L1 and PD-1 in peripheral T-cell lymphoma,which was closely related with elevated LDH in peripheral blood ,poor response rate and shorter OS. So they could be the factors of worse effect of chemotherapy and poor prognosis. Related Articles | Metrics

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Analysis of clinical features and risk factors for adult nephrotic syndrome with pulmonary thromboembolism FENG Wei, WANG Yifeng， TANG Rongwei, LIAO Yunhua 2016, 44 (3):  353-356.  doi: 10.11958/58741 Abstract ( 907 )   PDF (331KB) ( 3846 )   Objective To investigate the clinical features and risk factors of adult nephrotic syndrome with pulmonary thromboembolism (PTE). Methods Sixty patients diagnosed with nephrotic syndrome and clinically suspected with PTE were enrolled in this retrospective study. Patients were divided into PTE group (n=32) and no-PTE group (n=28) according to the results of computed tomographic pulmonary angiography (CTPA). The single factor analysis and Logistic repression analysis were used to analyse risk factors including age, gender, onset time, clinical symptoms, laboratory examination and pathological types. According to the independent risk factors, the receiver-operating characteristic curve (ROC curve) was used to determine PTE threshold value based on the evaluation index in nephrotic syndrome. Results Single factor analysis showed that there were significant differences in disease duration, hemoglobin, serum albumin, total cholesterol, low-density lipoprotein cholesterol, D-dimer and physical examination in P2 hyperthyroidism between two groups (P < 0.05). Logistic regression analysis showed that D-dimer was independent risk factor of PTE. The analysis of ROC curve indicated that D-dimer optimal threshold was 1 015.50 μg/L. Conclusion D-dimer is an independent risk factor of PTE in patients with nephrotic syndrome. When D-dimer is greater than 1 015.50 μg/L, should pay attention to the occurrence of PTE. Related Articles | Metrics

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Research of the risk that H-type hypertension leading to left ventricular remodeling REN Zhijing ZHANGJing CONG Hongliang 2016, 44 (3):  356-359.  doi: 10.11958/20150103 Abstract ( 1002 )   PDF (333KB) ( 3784 )   Abstrac Objective: To study If there is a synergistic effect on leading to left ventricular remodelingwhen the patient both suffer from homocystinemia and hypertension.Methord:Choose 275 TianjinChest Hospital Cardiology in-patients and divide them into 4 groups based on their plasma homocysteine level and whether suffer from hypertension。Then analyse and compare left ventricular relative thickness、Left ventricular mass index（LVMI）、the proportion of left ventricular remodeling in the 4 groups. Results:The H- hypertension Group has a higher level in left ventricular relative thickness、Left ventricular mass index（LVMI）、the proportion of left ventricular remodeling copared to the other 3 groups[（0.45±0.11）Vs（0.43±0.09）Vs（0.42±0.15）Vs（0.33±0.12），（130.12±55.73）Vs（110.17±58.20）Vs（110.35±44.86）Vs（93±20.33），（40.2%）Vs（22.7%）Vs（24.3%）Vs（3.1%），p＜0.05]. Conclusion: Homocysteine and systolic pressure are both independent risk factors for left ventricular remodeling and they have a synergistic effect on leading to left ventricular remodeling.Which stress out that we should control blood pressure as well as reduce plasma homocysteine level Related Articles | Metrics

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The superiority to use specific value of lactic and central venous oxygen saturation in shock patients WANG Ling, YANG Yongling, WANG Wanling 2016, 44 (3):  360-362.  doi: 10.11958/59083 Abstract ( 1006 )   PDF (329KB) ( 3837 )   Objective:To investigate the significance of the application value of lactic（Lac） and central venous oxygen saturation(ScvO2 )in shock patients . Methods: Selection the shock patients that stay in the department of emergency intensive care unit(EICU) in our hospital fron January 2013 to April 2015.Monitoring the lactic and ScvO2 at arrive hospital,After 6 hours moniting lactic again to calculate the lactate clearance rate(6h-LCR). Calculate acate physicalogy and chronic health evalauation Ⅱ(APACHE Ⅱ) score within 24 hours .The 28 days after stay hospital is the end ling of study.Patients are divided into generation group and death group. Comparing the difference of Lac/ScvO2 between the two groups , Analyse the correlation of Lac/ScvO2 with death, Lac level , APACHE Ⅱ score. Draw the receiver operating characteristic curve (ROC curve) analysis the ability of Lac/ScvO2 , Lac, 6h-LCR, ScvO2, APACHE Ⅱ score to judge happen derth with shock patients. The end compares the advantages of them. Results: The gender and age of two groups are no statistical difference (P > 0.05).The Lac/ScvO2 of Survival group is lower than the death group (P < 0.05). Lac, ScvO2, 6h-LCR, APACHE Ⅱ score have obvious difference (P < 0.05). Lac/ScvO2 with Lac and APACHE Ⅱ scores were positively correlated, (r = 0.901, r = 0.659, P < 0.01), Lac/ScvO2 related to happen death (r = 0.512, P < 0.01). The AUC of Lac/ScvO2 is 0.844 (0.751, 0.751), The cutoff value is 5.91, Sensitivity is 86.70%, Specificity is 67.60%, OR value is 16.25. Relative to the Lac, ScvO2, 6h-LCR, APACHE Ⅱ score ,The AUC and sensitivity of Lac/ScvO2 is the highest. Conclusions: Lac/ScvO2 is a better indicators for shock patients to evaluation the degree of the severity of illness and prognosis. Lac/ScvO2 is the best than Lac, ScvO2, 6h-LCR and APACHE Ⅱ score to judge prognosis in shock patients. Related Articles | Metrics

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Monitoring microembolic signals in patients with brain stem infarction RONG Yanhong, ZHAO Kun△, SU Hongjun, QI Jinlong 2016, 44 (3):  363-366.  doi: 10.11958/20150054 Abstract ( 1036 )   PDF (280KB) ( 3994 )   Background: Monitoring microembolic signals (MES) in patients with brain stem infarction has not been reported in the literature. This study aimed to investigate the relation between the presence of MES and vertebrobasilar artery (VBA) stenosis. Methods: The study included 156 patients with acute brain stem infarction who underwent detection of MES at the VBA through the occipital window using transcranial Doppler (TCD) ultrasonography. Results: MES were present in 20 (12.8%) patients. Clinical characteristics and laboratory parameters of the MES-positive and MES-negative groups did not differ significantly. Intracranial VBA stenosis was found to be an independent risk factor for MES [odds ratio (OR), 9.73; 95% confidence interval (CI), 1.34–81.46; p=0.038]. Patients with severe VBA stenosis had a higher prevalence of MES than those with moderate VBA stenosis (OR, 9.76; 95% CI, 1.53-80.12; p=0.040). VBA stenosis was found in 86 patients. The MES-positive group had more infarctions than the MES-negative group (p=0.014). Conclusion: VBA stenosis is the primary source of microemboli in patients with acute brain stem infarction. Microemboli originating from VBA stenosis are the cause of multiple infarctions. Related Articles | Metrics

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Assessment ofthe Efficacy of Cardiac Resynchronization Therapy Using Real-TimeThree—Dimensional Echocardiography and speckle tracking imaging in Patients with Chronic Heart Failure LUO Yongjuan, GUAN Xin, ZHU Yanbo 2016, 44 (3):  367-369.  doi: 10.11958/59111 Abstract ( 777 )   PDF (1086KB) ( 3654 )   Objective To assess the efficacy of cardiac resynchronization therapy using real-time three-dimensionsl echocflrdiography and speckle trackingimaging in patients with chronic heart failure. Methods Thirty patients with chronic heart failure were examined by RT-3DE and STI before CRT and 3，6months after CRT.Measured LVEDV、LVESV and LVEF.RT-3DE parameters were the dispersion of time to minimum regional volumefor 16 segments(Tmsvl6SD) and the ratio of Tmsvl6-SD to R-R interval and the maximum time difference(Tmsvl6-Dif).STl parameters were the ratios of standard deviation of the time to peak longitudinal radial and circumferential strainat to R-R intervaI(Tls-16SD% Trs-16SD%，Tcs-16SD%) from the beginning of Q wave of electrocardiography to the point atminimal systolic Volume of all 16segments and the maximum time difference 。Results The LVEDVand LVESVof 3，6months after CRT were smaller than that before CRT,The LVEF raised . The Tmsv-16SD%、Tmsvl6Dif%、Tls-l6SD%、Trs-l6SD%、Tcs-l6SD% and T-Dif% of 3，6months after CRT reduced than that before CRT, (P<0.05). The Tmsv-16SD% 、Tmsvl6Dif%、Trs-l6SD%、Tcs-l6SD% were negatively associated with LVEF. Conclusion Real-time three-dimensionsl echocflrdiography and speckle tracking imaging represents a promising novel technique for assessment of global LV function and dyssynchr. Related Articles | Metrics

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The facial nerve functional recovery law after acoustic neuroma operation and the influence of tumor size on it DING Weiliang1, PU Ke2, WANG Hong2△ 2016, 44 (3):  370-372.  doi: 10.11958/20150045 Abstract ( 1160 )   PDF (364KB) ( 3770 )   Objective To explore the facial nerve functional recovery law after resection of acoustic neuroma .And studied the influence of tumor size on postoperative facial nerve function. Methods According to the House-Brackman (HB) facial nerve function classification methods, grading 89 patients with acoustic neuroma after microsurgical resection with the retrosigmoid approach and facial nerve preservation. They were observed, follow-up with call and retrospectively analyzed. Choosing immediately , 15 days, 45 days , 3 months, 6 months, 12 months and more than 12 months after operation for the point .To explore the rule of the postoperative facial nerve functional recovery. And according to the tumor diameter < 30 mm, 30 ~ 40 mm, and ≥40mm divided into three groups. Compared the patients facial nerve function in different groups with early postoperative (within 15 days) and long-term (more than 12 months). Results The facial nerve function worst in 15 days after operation (excellence rate was 52.81%), but in postoperative 3 months most returning (excellence rate reached 80.90% ), for the most part can be restored to excellence in postoperative 12 months (excellence rate was 91.01% ), and the facial nerve recovery more smoothly after postoperative 12 months. Patients whoes tumor diameter < 30 mm have better excellence in early postoperative (rate was 82.61%) and long-term (rate was 100%). Tumor size has remarkable effect on facial nerve function in the early postoperative (χ2 = 23.34, P = 23.34, P < 0.05), and long-term (χ2 = 14.46, P = 14.46, P < 0.05) . And they are correlation, the early stage (r = 0.476, P = 0.000, P < 0.05) and long-term (r = 0.379, P = 0.000, P < 0.05) . The tumor more larger ,the postoperative facial nerve function in the early and long-term more poorer. Conclusion The retrosigmoid approach and facial nerve preservation in patients who have acoustic neuroma ,the facial nerve function in early postoperative (within 15 days) may appear deterioration, but most can be recovery in postoperative 3 months, and almost all can be recovery in 12 months. The postoperative facial nerve function closely related with the size of the tumors not only in the early postoperative (within 15 days) but also in the long-term (> 12 months), which is one of the important factors influencing the postoperative facial nerve function. Related Articles | Metrics

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The investigation and analysis ondiarrhea incidence and treatment behavior in Tianjin community GAO Lu, SUN Zhong 2016, 44 (3):  373-376.  doi: 10.11958/58528 Abstract ( 753 )   PDF (350KB) ( 3741 )   Objective To investigate the symptoms of diarrhea and the behavior of medical treatment after diarrhea in 2013 in Tianjin City, and provid background information for the future population monitoring. Methods A stratified sampling method was used to investigate the symptoms, medical treatment and self medication behavior of 4028 residents in Tianjin. Results The number of people who had diarrhea in 2013 was 324, and the incidence rate of diarrhea was 8.04%.Diarrhea was mainly occurred in May-October (n=241, 74.38%). The incidence of diarrhea was the highest in 60-74 years old group, followed by 75- years old and 0-14 years old groups. The incidence of diarrhea was the lowest in 15-29 years old grouop. A total of 121 patients with diarrhea chose to go to hospital (47.08%), 78 patients chose to buy medicine for treatment (30.35%) and 58 patients did not do any disposal (22.57%). Among the patients with diarrhea, the highest proportion of patients chose to go to the first grade and the following medical institutions (68.59%, 83/121). There were 14.05% (17/121) and 17.36% (21/121) patients chose to go to the second and the third levels of medical institutions for the treatment. The av⁃ erage cost for patients with diarrhea was 60 (20, 200) yuan. The purchasing expenditure median was 20 (11,50) yuan. The medical spending was more than the purchase of medicines (Z=2.412, P < 0.05). The average cost of medical treatment was more higher in the second and the third levels of medical institutions than that of the first medical institution (Z=50.709， P＜ 0.05). The average cost of medical treatment was in turn increased for patients with diarrhea treated in Baodi county, Xiqing county, Hangu county and Heping county (Z=74.282， P＜ 0.05). There was no statistical significance in medical expenses be⁃ tween patients with different ages and patient with or without medical insurance. Conclusion There are high incidence of diarrhea in patients under 15 years old and patients over 60 years old. Residents suffering from diarrhea mainly choose their own medicine and the treatment in the first and following medical institutions. Related Articles | Metrics

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The overview of the pattern of the mild hypothermia in brain injury animal model LIU Chenglong, CHEN Chong, SUN Hongtao 2016, 44 (3):  377-380.  doi: 10.11958/20150140 Abstract ( 750 )   PDF (374KB) ( 3627 )   Abstract: Brain injury is a major cause of worldwide mortality and disability, and it has brought a heavy burden to the society and the family. The hypothermia of brain injury animal models can provide new intervention and therapeutic measures for the brain injury patients. The role of brain protection has been demonstrated in a large number of animal models of brain injury in mild hypothermia experiments. However, the pattern of mild hypothermia in brain injury animals, at present, is not fully delineated, and the optimal pattern, such as the duration, induced method and the topgallant temperature is still unclear. Therefore, this paper summarizes the experimental method of mild hypothermia in brain injury animal model. Related Articles | Metrics

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Research progress of antiviral treatment in preventing mother-to-infant transmission of hepatitis B virus LIANG Qian, LI Ni, SUN Yanyan, SONG Shurong 2016, 44 (3):  381-384.  doi: 10.11958/58999 Abstract ( 676 )   PDF (296KB) ( 3892 )   Hepatitis B virus (HBV) infection is a worldwide health problem and vertical transmission is the main source of chronic infection in Asian countries. Breakthrough perinatal HBV transmission occurs in 10%- 15% of infants born to pregnatal women with high viral load even with appropriate postexposure immunoprophylaxis. Management of chronic hepatitis B (CHB) during pregnancy remains a challenge, with unique issues that involve prevention of mother-to-child transmis⁃ sion (MTCT) and safe use of antiviral therapy. Previous studies indicate that antiviral therapy with analogs (Lamivudine, Telbivudine or Tenofovir) may prevent vertical transmission during late pregnancy in highly viremic mothers. This article summarized relevant studies that antiviral therapy in preventing MTCT of hepatitis B virus. Related Articles | Metrics