Abstract: Abstract: Objective　To study the impact of Zishen Yugui decoction on serum anti-Müllerian hormone (AMH), estradiol (E2) and follicle stimulating hormone (FSH) in patients with premature ovarian insufficiency (POI). Methods　A total of 157 patients with POI were randomized into 3 groups: the Chinese medicine group (51 cases treated with Zishen Yugui decoction), the Femoston group (54 cases treated with Femoston) and the combined treatment group (52 cases treated with both Femoston and Chinese medicine). The total efficiency was analyzed compared between the 3 groups.The serum levels of AMH, E2 and FSH before and after treatment were compared between the three groups.Besides, the situation of menstruation, pregnancy rate, endometrial thickness, ovarian volume, antral follicle count, ovarian peak systolic velocity (PSV) and RI before treatment and 4 weeks after treatment were compared between the 3 groups. Meanwhile, the incidence of adverse reaction was recorded. Results　Compared with the Chinese medicine group and the Femoston group, the total efficiency was higher in the combined treatment group (P＜0.05).In the 4th and 12th week of treatment, the serum levels of AMH and E2 were higher in the combined treatment group than those of Chinese medicine group and the Femoston group, and the FSH level was lower in the combined treatment group (P＜0.05). At 4-week treatment, the menstrual cycle, menstrual period and menstrual volume were longer/more in the combined treatment group than those of Chinese medicine group and the Femoston group (P＜0.05), and the pregnancy rate was higher in the combined treatment group (P＜0.05). Compared with the Chinese medicine group and the Femoston group, the endometrial thickness and resistance index (RI) were smaller, and the ovarian volume, antral follicle count and ovarian PSV were larger in the combined treatment group (P＜0.05). No significant adverse reactions were found in the 3 groups.Conclusion　Menstrual disorders, vaginal ultrasound parameters, pregnancy rate, and serum levels of AMH, E2 and FSH can be effectively improved through treatment with Zishen Yugui decoction in patients with POI. The treatment method is safe and reliable.

Key words: primary ovarian insufficiency, estradiol, follicle stimulating hormone, anti-mullerian hormone, ZishenYugui decoction