Abstract: Objective　To investigate the effects of naringin on the differentiation and polarization of osteoclast. Methods　RAW264.7 cells were induced using culture media containing different concentrations of naringin (0 μg/L, 2 μg/L, 20 μg/L, 200 μg/L). The results of induction and expression levels of CK, MMP-9, integrin β3, c-src and p-src were compared between groups. Results　Compared with the control group, TRAP staining positive cell number was significantly reduced in the experimental group (P＜0.05). Compared with the control group, the mRNA expression levels of CK, MMP-9, integrin β3 and c-src were significantly reduced in 20 μg/L and 200 μg/L groups (P＜0.05), and the protein expression levels of CK, MMP-9, integrin β3 and p-src were also significantly reduced (P＜0.05). Conclusion　Naringin might inhibit the differentiation and polarization of osteoclast, and the effects might be related to the down regulation of integrin β3, c-src and p-src expressions.

Key words: osteoclasts, osteoporosis, cell differentiation, genes, src, integrin beta3, cathepsin K, matrix metalloproteinase 9, naringin