Impact of age on the biological properties of human adipose tissue-derived mesenchymal stromal cells

doi:10.11958/20220808

Abstract

Abstract:

Objective To investigate the impact of donor age on biological properties of human adipose tissue-derived mesenchymal stromal cells (ADSCs). Methods Subcutaneous adipose tissue and peripheral blood sample were collected after abdominal surgery. They were divided into the child group (n=10), the adult group (n=10) and the >50 years old group (n=10). ADSCs were isolated and cultured. Cell immunophenotypic characteristics were determined by flow cytometry. The proliferation and migration cell index (CI) were monitored with a Real-Time Cell Analyzer. Multilineage differentiation potential was investigated by evaluating the induction response. The mRNA expression of osteopontin (OPN) and peroxisome proliferator-activated receptor-γ (PPAR-γ) from ADSCs were determined by reverse transcription-polymerase chain reaction. ADSCs were co-cultured with peripheral blood mononuclear cells (PBMCs) in presence of concomitant phytohemagglutinin stimulation. Enzyme linked immunosorbent assay was performed to detect the IFN-γ levels in culture supernatants. Results After 3 passages, ADSCs from the three groups showed typical spindle-shaped, and all expressed typical mesenchymal stem cell (MSCs) surface markers CD90, CD105 and CD73. The proliferation, migration CI, and the expression levels of OPN mRNA were significantly increased in ADSCs derived from the child group compared with those in the adult group and the >50 years old group (P<0.05). ADSCs derived from the child group significantly decreased the secretion of IFN-γ in culture supernatants compared with the adult group and the >50 years old group (P<0.05). Conclusion ADSCs derived from children have biological advantages in the proliferation, migration, multilineage differentiation potential and immunosuppressive effects in vitro.

Key words: adipose tissue, mesenchymal stem cell transplantation, immunity,cellular, cell differentiation, age factors

CLC Number:

R392.12

Figures/Tables 6

Fig.1 Morphologic characteristics of ADSCs from three age groups (×200)

Fig.2 Morphologic characteristics of ADSCs after Gemsa staining (×400)

Fig.3 Detection of surface markers of ADSCs detected by flow cytometry

Tab.1 Comparison of cell proliferation and migration cell index of ADSCs derived from three age groups

组别	增殖CI	迁移CI
儿童组	2.65±0.18	2.34±0.17
成年组	2.43±0.15^a	1.92±0.14^a
>50岁组	2.32±0.15^a	1.78±0.20^a
F	11.580^＊＊	29.460^＊＊

Fig.4 Adipogenic and osteogenic induction differentiation of isolated ADSCs derived from three age groups (×200)

Tab.2 Comparison of PPAR-γ,OPN mRNA expression in ADSCs and IFN-γ secretion in PBMCs from three age groups

组别	PPAR-γ mRNA	OPN mRNA	IFN-γ（ng/L）
儿童组	0.55±0.11	0.58±0.07	638.5±40.4
成年组	0.57±0.08	0.50±0.06^a	687.4±44.8^a
>50岁组	0.63±0.08	0.36±0.07^ab	740.6±36.6^ab
F	2.066	26.539^＊＊	15.701^＊＊

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