Abstract:

Objective To explore the mechanism of miR-891a-5p regulating the proliferation, apoptosis, migration and invasion of colon cancer cells through cytoplasmic polyadenylation element binding protein 1 (CPEB1). Methods Real-time fluorescence quantitative reverse transcription polymerase chain reaction (qPCR) was used to detect the expression of miR-891a-5p in colon cancer tissue, paracancer tissue, human normal colon epithelial cells and human colon cancer cells. LOVO cells were divided into the group A (without any treatment), the group B (transfected with anti-miR-con), the group C (transfected with anti-miR-891a-5p), the group D (co-transfected with anti-miR-891a-5p and si-con), group E (co-transfected with anti-miR-891a-5p and si-CPEB1), the group F (transfected with miR-con) and group G (transfected with miR-891a-5p). Cell proliferation rate was detected by CCK-8 assay, and apoptosis rate was detected by Annexin V-FITC/PI assay. Cyclin D1, Pro-caspase-3, C-caspase-3, N-cadherin, E-cadherin and Vimentin were detected by Western blot assay. Transwell assay was used to detect cell migration and invasion. Double luciferase reporter gene assay was used to detect the fluorescence activity of cells. Results The expression of miR-891a-5p in colon cancer tissue was significantly increased compared with that in normal colon epithelial cells (P<0.05). Compared with the group B, protein expressions of miR-891a-5p, Cyclin D1, N-cadherin and Vimentin were decreased in the group C, and cell proliferation rate, migration and invasion number and protein expressions of C-caspase-3, CPEB1 and E-cadherin and apoptosis rate were increased (P<0.05). Compared with the group D, the protein expressions of miR-891a-5p, Cyclin D1, N-cadherin and Vimentin, proliferation rate, migration and invasion number of cells were increased in the group E, while the protein expressions of C-caspase-3, CPEB1 and E-cadherin and apoptosis rate were decreased (P<0.05). There were six consecutive complementary binding sites between miR-891a-5p and CPEB1 at the 3 'UTR. Compared with the group F, the fluorescence activity and CPEB1 protein expression of WT-CPEB1 were decreased in the group G. Compared with the group B, the fluorescence activity of WT-CPEB1 was increased in the group C (P<0.05). Conclusion miR-891a-5p can promote the proliferation, migration and invasion of colon cancer cells and inhibit apoptosis, which may be related to the targeted inhibition of CPEB1.

Key words: colonic neoplasms, cell proliferation, apoptosis, miR-891a-5p, CPEB1